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Cellosaurus RAW 264.7 (CVCL_0493)

[Text version]

Cell line name RAW 264.7
Synonyms RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
Accession CVCL_0493
Resource Identification Initiative To cite this cell line use: RAW 264.7 (RRID:CVCL_0493)
Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Doubling time: 11 hours (from cell counting), 12 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); ~30 hours (CLS).
Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
Omics: Metabolome analysis.
Omics: Phagosome quantitative phosphoproteome analysis.
Omics: Phagosome analysis by proteomics.
Omics: SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (17) than that of NIST (18) due to a change in the marker motif (personal communication of Almeida J.L.).
Misspelling: RAW 267.4; Occasionally.
Misspelling: Raw 267.4; Occasionally.
Misspelling: RAW267.4; Occasionally.
Misspelling: RAW 274; Occasionally.
Misspelling: RAW-274; Occasionally.
Misspelling: RAW274; Occasionally.
Derived from sampling site: Ascites.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_4478 (RAW 264)
Children:
CVCL_KB45 (CellSensor NFkB-bla RAW 264.7)CVCL_DD02 (EA13.5)CVCL_B417 (M9A)
CVCL_6517 (RAW 264.7 gammaNO(-))CVCL_UL72 (RAW 264.7 LRRK2 KO)CVCL_UL71 (RAW 264.7 LRRK2 parental)
CVCL_UL73 (RAW 264.7 LRRK2 T1348N mut)CVCL_ZD89 (RAW 264.7 shRNA-RSK2)CVCL_6717 (RAW 264.7/LR5)
CVCL_X594 (RAW-Blue)CVCL_X595 (RAW-Blue ISG)CVCL_F681 (RAW-D)
CVCL_X596 (RAW-Lucia ISG)CVCL_9W02 (RAW-TT10)CVCL_1P00 (RAW264-TG3)
Sex of cell Male
Age at sampling Adult
Category Cancer cell line
STR profile Source(s): PubMed=23430347; PubMed=31220119

Markers:
Mouse STR 1-115,16
Mouse STR 1-217
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-222.3
Mouse STR 5-514
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 7-125.2
Mouse STR 8-113
Mouse STR 9-215
Mouse STR 11-217
Mouse STR 12-116
Mouse STR 13-116.2
Mouse STR 15-322.3
Mouse STR 17-214,16
Mouse STR 18-318
Mouse STR 19-214
Mouse STR X-124

Run an STR similarity search on this cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=RAW264.7
http://www.infarktforschung.de/macrophages_cell_lines.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/r/cell-lines-detail-325.html
http://www.cellresource.cn/fdetail.aspx?id=146
http://www.cellresource.cn/fdetail.aspx?id=739
http://www.cellresource.cn/fdetail.aspx?id=899
Publications

PubMed=212198; DOI=10.1016/0092-8674(78)90101-0
Raschke W.C., Baird S., Ralph P., Nakoinz I.
Functional macrophage cell lines transformed by Abelson leukemia virus.
Cell 15:261-267(1978)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=19057837; DOI=10.1007/s00774-008-0018-6
Vincent C., Kogawa M., Findlay D.M., Atkins G.J.
The generation of osteoclasts from RAW 264.7 precursors in defined, serum-free conditions.
J. Bone Miner. Metab. 27:114-119(2009)

PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
The phagosomal proteome in interferon-gamma-activated macrophages.
Immunity 30:143-154(2009)

PubMed=22735334; DOI=10.1038/msb.2012.21
Bordbar A., Mo M.L., Nakayasu E.S., Schrimpe-Rutledge A.C., Kim Y.-M., Metz T.O., Jones M.B., Frank B.C., Smith R.D., Peterson S.N., Hyduke D.R., Adkins J.N., Palsson B.O.
Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.
Mol. Syst. Biol. 8:558-558(2012)

DOI=10.5897/IJBMBR2013.0154
Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847-847(2014)

PubMed=25504905; DOI=10.1002/pmic.201400431
Guo M., Hartlova A., Dill B.D., Prescott A.R., Gierlinski M., Trost M.
High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.
Proteomics 15:3169-3174(2015)

PubMed=29889899; DOI=10.1371/journal.pone.0198943
Taciak B., Bialasek M., Braniewska A., Sas Z., Sawicka P., Kiraga L., Rygiel T., Krol M.
Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages.
PLoS ONE 13:E0198943-E0198943(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

Cross-references
Cell line collections AddexBio; C0003008/444
ATCC; TIB-71
BCRC; 60001
BCRJ; 0212
CCTCC; GDC0143
CLS; 400319/p462_RAW-2647
ECACC; 91062702
IBRC; C10072
ICLC; ATL02001
IZSLER; BS TCL 177
KCB; KCB 200603YJ
KCLB; 40071
NCBI_Iran; C639
Cell line databases/resources CLDB; cl4110
CLDB; cl7129
CCRID; 3111C0001CCC000146
CCRID; 3131C0001000800013
CCRID; 3142C0001000000131
FCS-free; 225-5-468-2-3-3
Lonza; 674
TOKU-E; 2976
TOKU-E; 4095
Ontologies BTO; BTO:0003292
CLO; CLO_0008753
CLO; CLO_0051424
EFO; EFO_0001231
MCCL; MCC:0000390
MeSH; D000067996
Biological sample resources BioSample; SAMN11397665
eagle-i; hawaii.eagle-i.net/i/0000012b-5667-653a-2f73-b43980000000
Chemistry resources ChEMBL-Cells; CHEMBL3307977
ChEMBL-Targets; CHEMBL612557
Metabolomic databases MetaboLights; MTBLS23
Other Wikidata; Q28335461
Proteomic databases PRIDE; PXD000984
PRIDE; PXD001293
PRIDE; PXD001905
PRIDE; PXD001935
PRIDE; PXD003545
Entry history
Entry creation04-Apr-2012
Last entry update06-Sep-2019
Version number27