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Cellosaurus Sf21 (CVCL_0518)

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Cell line name Sf21
Synonyms SF21; Sf-21; SF-21; IPLB-SF-21-AE; IPLB-Sf21-AE; IPLB-SF21-AE; IPLB-SF 21AE; IPLB-Sf21AE; IPLB-SF21AE; IPLB-SF-21; IPLB-Sf-21; IPLB-SF 21; IPLB-Sf21; IPLB-SF21
Accession CVCL_0518
Resource Identification Initiative To cite this cell line use: Sf21 (RRID:CVCL_0518)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Doubling time: 26-30 hours (PubMed=68913); ~3 days (DSMZ); 24 hours (ECACC).
Misspelling: IPBL-SF-21AE; Occasionally.
Misspelling: IPBL-SF-21; Occasionally.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Children:
CVCL_Z451 (IBL-Sf-21AE-Cl 3)CVCL_Z452 (IBL-Sf-21AE-Cl 5)CVCL_Z453 (IBL-Sf-21AE-Cl 9)
CVCL_2074 (Sf158)CVCL_JX37 (Sf21 TiterHigh AC free)CVCL_0549 (Sf9)
Sex of cell Female
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Sf21

PubMed=68913; DOI=10.1007/BF02615077
Vaughn J.L., Goodwin R.H., Tompkins G.J., McCawley P.
The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).
In Vitro 13:213-217(1977)

Volkman L.E., Goldsmith P.A.
Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro.
Appl. Environ. Microbiol. 44:227-233(1982)

Mitsuhashi J.
Nutritional requirements of insect cells in vitro.
(In) Invertebrate cell system applications, Vol. I; Mitsuhashi J. (eds.); pp.3-20; CRC Press; Boca Raton (1989)

PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

Li L.-L., Chen Q.-H.
Storage of insect cell lines by a simple method.
Hua Zhong Shi Fan Da Xue Xue Bao Zi Ran Ke Xue Ban 29:511-512(1995)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8
Lynn D.E.
Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses.
J. Invertebr. Pathol. 82:129-131(2003)

PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)

PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

Cell line collections CCTCC; GDC0020
ECACC; 05022801
KCB; KCB 201008YJ
NCBI_Iran; C503
Cell line databases/resources CLDB; cl2906
CCRID; 3142C0001000000020
Lonza; 1183
Ontologies BTO; BTO:0001196
CLO; CLO_0008987
MCCL; MCC:0000416
Biological sample resources eagle-i; hawaii.eagle-i.net/i/0000012b-5661-2f63-2f73-b43980000000
Other Wikidata; Q2276068
Entry history
Entry creation04-Apr-2012
Last entry updated24-May-2019
Version number18