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Cellosaurus Sf21 (CVCL_0518)

Cell line name Sf21
Synonyms SF21; Sf-21; SF-21; IPLB-SF-21-AE; IPLB-Sf21-AE; IPLB-SF21-AE; IPLB-SF 21AE; IPLB-Sf21AE; IPLB-SF21AE; IPLB-SF-21; IPLB-Sf-21; IPLB-SF 21; IPLB-Sf21; IPLB-SF21
Accession CVCL_0518
Resource Identification Initiative To cite this cell line use: Sf21 (RRID:CVCL_0518)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Doubling time: 26-30 hours (PubMed=68913); 33 +- 1 hours (Note=In static culture), 26 +- 1 hours (Note=In suspension culture) (PubMed=10585188); ~3 days (DSMZ=ACC-119); 24 hours (ECACC=05022801).
Misspelling: IPBL-SF-21AE; Note=Occasionally.
Misspelling: IPBL-SF-21; Note=Occasionally.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Children:
CVCL_C2TG (IBL-Sf-21AE-Cl 1)CVCL_C2TJ (IBL-Sf-21AE-Cl 10)CVCL_C2TK (IBL-Sf-21AE-Cl 13)
CVCL_C2TM (IBL-Sf-21AE-Cl 14)CVCL_C2TN (IBL-Sf-21AE-Cl 15)CVCL_C2TL (IBL-Sf-21AE-Cl 18)
CVCL_C2TH (IBL-Sf-21AE-Cl 2)CVCL_Z451 (IBL-Sf-21AE-Cl 3)CVCL_Z452 (IBL-Sf-21AE-Cl 5)
CVCL_C2TI (IBL-Sf-21AE-Cl 7)CVCL_Z453 (IBL-Sf-21AE-Cl 9)CVCL_A7NB (Sf-21-CB)
CVCL_2074 (Sf158)CVCL_JX37 (Sf21 TiterHigh AC free)CVCL_A2IN (Sf21-HT)
CVCL_0549 (Sf9)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Sf21

PubMed=68913; DOI=10.1007/BF02615077
Vaughn J.L., Goodwin R.H., Tompkins G.J., McCawley P.
The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).
In Vitro 13:213-217(1977)

PubMed=16346059; DOI=10.1128/AEM.44.1.227-233.1982
Volkman L.E., Goldsmith P.A.
Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro.
Appl. Environ. Microbiol. 44:227-233(1982)

Mitsuhashi J.
Nutritional requirements of insect cells in vitro.
(In) Invertebrate cell system applications, Vol. I; Mitsuhashi J. (eds.); pp.3-20; CRC Press; Boca Raton (1989)

PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=1369220; DOI=10.1021/bp00017a003
Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

Li L.-L., Chen Q.-H.
Storage of insect cell lines by a simple method.
Hua Zhong Shi Fan Da Xue Xue Bao. Zi Ran Ke Xue Ban 29:511-512(1995)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10475266; DOI=10.1007/s11626-999-0010-z
Gundersen-Rindal D.E., Lynn D.E., Dougherty E.M.
Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA.
In Vitro Cell. Dev. Biol. Anim. 35:111-114(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Keith M.B., Farrell P.J., Iatrou K., Behie L.A.
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=10852353; DOI=10.1290/1071-2690(2000)036<0271:IVHRSW>2.0.CO;2
Kariuki C.W., Mcintosh A.H., Goodman C.L.
In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera).
In Vitro Cell. Dev. Biol. Anim. 36:271-276(2000)

PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8
Lynn D.E.
Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses.
J. Invertebr. Pathol. 82:129-131(2003)

PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2
Grasela J.J., McIntosh A.H.
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)

PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)

PubMed=16409117; DOI=10.1290/0412083R.1
McIntosh A.H., Grasela J.J., Popham H.J.R.
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)

PubMed=22797937; DOI=10.1007/s10158-012-0138-5
Jenson L.J., Paulson S.L., Bloomquist J.R.
Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.
Invertebr. Neurosci. 12:119-127(2012)

PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H.-L., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

Cell line collections (Providers) CCTCC; GDC0020
ECACC; 05022801
KCB; KCB 2010008YJ
NCBI_Iran; C503
Cell line databases/resources CLO; CLO_0008987
MCCL; MCC:0000416
CLDB; cl2906
DSMZCellDive; ACC-119
ICLDB; iplb-sf21
Lonza; 1183
Anatomy/cell type resources BTO; BTO:0001196
Chemistry resources ChEMBL-Cells; CHEMBL4106362
PubChem_Cell_line; CVCL_0518
Encyclopedic resources Wikidata; Q2276068
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number28