ID   Sf21
AC   CVCL_0518
SY   SF21; Sf-21; SF-21; IPLB-SF-21-AE; IPLB-Sf21-AE; IPLB-SF21-AE; IPLB-SF 21AE; IPLB-Sf21AE; IPLB-SF21AE; IPLB-SF-21; IPLB-Sf-21; IPLB-SF 21; IPLB-Sf21; IPLB-SF21
DR   BTO; BTO:0001196
DR   CLO; CLO_0008987
DR   MCCL; MCC:0000416
DR   CLDB; cl2906
DR   CCRID; 4201INS-CCTCC00020
DR   DSMZ; ACC-119
DR   eagle-i;
DR   ECACC; 05022801
DR   KCB; KCB 2010008YJ
DR   Lonza; 1183
DR   NCBI_Iran; C503
DR   Wikidata; Q2276068
RX   CelloPub=CLPUB00177;
RX   CelloPub=CLPUB00492;
RX   PubMed=68913;
RX   PubMed=1367489;
RX   PubMed=1369220;
RX   PubMed=8314732;
RX   PubMed=8799737;
RX   PubMed=10585188;
RX   PubMed=10777062;
RX   PubMed=10852353;
RX   PubMed=12623313;
RX   PubMed=15038778;
RX   PubMed=15926859;
RX   PubMed=16346059;
RX   PubMed=16409117;
RX   PubMed=22797937;
RX   PubMed=24672045;
RX   PubMed=29133148;
CC   Group: Insect cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Doubling time: 26-30 hours (PubMed=68913); 33 +- 1 hours (in static culture), 26 +- 1 hours (in suspension culture) (PubMed=10585188); ~3 days (DSMZ); 24 hours (ECACC).
CC   Misspelling: IPBL-SF-21AE; Occasionally.
CC   Misspelling: IPBL-SF-21; Occasionally.
CC   Derived from sampling site: Ovary.
OX   NCBI_TaxID=7108; ! Spodoptera frugiperda
SX   Female
AG   Pupal stage
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 23-09-21; Version: 22
RX   CelloPub=CLPUB00177;
RA   Li L.-L., Chen Q.-H.;
RT   "Storage of insect cell lines by a simple method.";
RL   Hua Zhong Shi Fan Da Xue Xue Bao. Zi Ran Ke Xue Ban 29:511-512(1995).
RX   CelloPub=CLPUB00492;
RA   Mitsuhashi J.;
RT   "Nutritional requirements of insect cells in vitro.";
RL   (In) Invertebrate cell system applications, Vol. I; Mitsuhashi J. (eds.); pp.3-20; CRC Press; Boca Raton (1989).
RX   PubMed=68913; DOI=10.1007/BF02615077;
RA   Vaughn J.L., Goodwin R.H., Tompkins G.J., McCawley P.;
RT   "The establishment of two cell lines from the insect Spodoptera
RT   frugiperda (Lepidoptera; Noctuidae).";
RL   In Vitro 13:213-217(1977).
RX   PubMed=1367489; DOI=10.1021/bp00007a002;
RA   Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.;
RT   "Expression of three recombinant proteins using baculovirus vectors in
RT   23 insect cell lines.";
RL   Biotechnol. Prog. 7:9-14(1991).
RX   PubMed=1369220; DOI=10.1021/bp00017a003;
RA   Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.;
RT   "Screening of insect cell lines for the production of recombinant
RT   proteins and infectious virus in the baculovirus expression system.";
RL   Biotechnol. Prog. 8:391-396(1992).
RX   PubMed=8314732; DOI=10.1007/BF02633986;
RA   Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L.,
RA   Wood H.A.;
RT   "Comparative recombinant protein production of eight insect cell
RT   lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993).
RX   PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x;
RA   McIntosh A.H., Grasela J.J., Matteri R.L.;
RT   "Identification of insect cell lines by DNA amplification
RT   fingerprinting (DAF).";
RL   Insect Mol. Biol. 5:187-195(1996).
RX   PubMed=10585188; DOI=10.1021/bp990119f;
RA   Keith M.B., Farrell P.J., Iatrou K., Behie L.A.;
RT   "Screening of transformed insect cell lines for recombinant protein
RT   production.";
RL   Biotechnol. Prog. 15:1046-1052(1999).
RX   PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2;
RA   Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.;
RT   "Expression of the green fluorescent protein carried by Autographa
RT   californica multiple nucleopolyhedrovirus in insect cell lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000).
RX   PubMed=10852353; DOI=10.1290/1071-2690(2000)036<0271:IVHRSW>2.0.CO;2;
RA   Kariuki C.W., Mcintosh A.H., Goodman C.L.;
RT   "In vitro host range studies with a new baculovirus isolate from the
RT   diamondback moth Plutella xylostella (L.) (Plutellidae:
RT   Lepidoptera).";
RL   In Vitro Cell. Dev. Biol. Anim. 36:271-276(2000).
RX   PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8;
RA   Lynn D.E.;
RT   "Comparative susceptibilities of twelve insect cell lines to infection
RT   by three baculoviruses.";
RL   J. Invertebr. Pathol. 82:129-131(2003).
RX   PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2;
RA   Grasela J.J., McIntosh A.H.;
RT   "Application of inter-simple sequence repeats to insect cell lines:
RT   identification at the clonal and tissue-specific level.";
RL   In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003).
RX   PubMed=15926859; DOI=10.1290/0412081.1;
RA   Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.;
RT   "Baculovirus-based expression of an insect viral protein in 12
RT   different insect cell lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005).
RX   PubMed=16346059; DOI=10.1128/AEM.44.1.227-233.1982;
RA   Volkman L.E., Goldsmith P.A.;
RT   "Generalized immunoassay for Autographa californica nuclear
RT   polyhedrosis virus infectivity in vitro.";
RL   Appl. Environ. Microbiol. 44:227-233(1982).
RX   PubMed=16409117; DOI=10.1290/0412083R.1;
RA   McIntosh A.H., Grasela J.J., Popham H.J.R.;
RT   "AcMNPV in permissive, semipermissive, and nonpermissive cell lines
RT   from Arthropoda.";
RL   In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005).
RX   PubMed=22797937; DOI=10.1007/s10158-012-0138-5;
RA   Jenson L.J., Paulson S.L., Bloomquist J.R.;
RT   "Induction and inhibition of an apparent neuronal phenotype in
RT   Spodoptera frugiperda insect cells (Sf21) by chemical agents.";
RL   Invertebr. Neurosci. 12:119-127(2012).
RX   PubMed=24672045; DOI=10.1128/JVI.00780-14;
RA   Ma H., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.;
RT   "Identification of a novel rhabdovirus in Spodoptera frugiperda cell
RT   lines.";
RL   J. Virol. 88:6576-6585(2014).
RX   PubMed=29133148; DOI=10.1016/j.pep.2017.11.002;
RA   Geisler C., Jarvis D.L.;
RT   "Adventitious viruses in insect cell lines used for recombinant
RT   protein expression.";
RL   Protein Expr. Purif. 144:25-32(2017).