Cellosaurus SK-N-BE(2) (CVCL_0528)

Cell line name SK-N-BE(2)
Synonyms SK-N-BE2; SKNBE(2); SKNBE-2; SKNBE2; SK-N-BE; SKNBE
Accession CVCL_0528
Resource Identification Initiative To cite this cell line use: SK-N-BE(2) (RRID:CVCL_0528)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
From: Memorial Sloan-Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan-Kettering Cancer Center Office of Technology Development; SK1980-530.
Doubling time: ~36 hours (DSMZ); 27 hours (COG).
Omics: Array-based CGH.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Neuroblastoma (NCIt: C3270)
Derived from metastatic site: Bone marrow.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_0529 (SK-N-BE(2)-C); CVCL_0167 (SK-N-BE(2)-M17)
Originate from same individual CVCL_9898 ! SK-N-BE(1)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): ATCC; COG; DSMZ; PubMed=25877200

Markers:
AmelogeninX,Y (ATCC; COG; PubMed=25877200)
X (DSMZ)
CSF1PO10
D13S31711
D16S5399,11
D18S5116 (COG)
14,16 (PubMed=25877200)
D19S43312,13
D21S1130,32.2 (COG)
30,31,32.2 (PubMed=25877200)
D2S133817,23
D3S135819
D5S81812
D7S8209,10
D8S117913,14
FGA22,25
Penta D13,14
Penta E14,18
TH016,7 (ATCC; COG; PubMed=25877200)
6 (DSMZ)
TPOX8,11
vWA18
Web pages http://www.cogcell.org/dl/NB_Data_Sheets/SK-N-BE2_Cell_Line_Data_Sheet_COGcell_org.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-565.html
https://www.mskcc.org/research-advantage/support/technology/tangible-material/sk-n-be-2-human-neuroblastoma-cell-line
Publications

DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683
Biedler J.L., Spengler B.A.
A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.
J. Natl. Cancer Inst. 57:683-695(1976)

PubMed=29704
Biedler J.L., Roffler-Tarlov S., Schachner M., Freedman L.S.
Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.
Cancer Res. 38:3751-3757(1978)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2535691
Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

DOI=10.1016/B978-0-12-333530-2.50006-X
Israel M.A., Thiele C.J.
Tumor cell lines of the peripheral nervous system.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York (1994)

PubMed=7838528
Cheng N.C., Van Roy N., Chan A., Beitsma M., Westerveld A., Speleman F., Versteeg R.
Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.
Oncogene 10:291-297(1995)

DOI=10.1007/0-306-46872-7_2
Thiele C.J.
Neuroblastoma.
(In) Human cell culture. Vol. 1. Cancer Cell Lines part 1; Masters J.R.W., Palsson B.O. (eds.); pp.21-53; Kluwer Academic Publishers; New York (1999)

PubMed=11550280; DOI=10.1002/gcc.1174
Van Roy N., Van Limbergen H., Vandesompele J., Van Gele M., Poppe B., Salwen H.R., Laureys G., Manoel N., De Paepe A., Speleman F.
Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.
Genes Chromosomes Cancer 32:126-135(2001)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177-177(2006)

PubMed=20631050; DOI=10.1101/gr.106252.110
Storlazzi C.T., Lonoce A., Guastadisegni M.C., Trombetta D., D'Addabbo P., Daniele G., L'Abbate A., Macchia G., Surace C., Kok K., Ullmann R., Purgato S., Palumbo O., Carella M., Ambros P.F., Rocchi M.
Gene amplification as double minutes or homogeneously staining regions in solid tumors: origin and structure.
Genome Res. 20:1198-1206(2010)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24792489; DOI=10.1007/s11060-014-1456-8
Farooqi A.S., Dagg R.A., Choi L.M.R., Shay J.W., Reynolds C.P., Lau L.M.S.
Alternative lengthening of telomeres in neuroblastoma cell lines is associated with a lack of MYCN genomic amplification and with p53 pathway aberrations.
J. Neurooncol. 119:17-26(2014)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

Cross-references
Cell line collections ATCC; CRL-2271
BCRJ; 0381
DSMZ; ACC-632
ECACC; 95011815
ICLC; HTL96015
KCB; KCB 201199YJ
Cell line databases/resources CLDB; cl4332
CLDB; cl4333
CCLE; SKNBE2_AUTONOMIC_GANGLIA
LINCS_LDP; LCL-1980
Ontologies BTO; BTO:0002696
CLO; CLO_0009052
MCCL; MCC:0000505
Biological sample resources BioSample; SAMN03472880
BioSample; SAMN03473451
Gene expression databases GEO; GSM554389
GEO; GSM563358
GEO; GSM692869
GEO; GSM827352
GEO; GSM887593
GEO; GSM888676
GEO; GSM1366411
GEO; GSM1670450
GEO; GSM2371255
GEO; GSM2394375
Metabolomic databases MetaboLights; MTBLS104
Polymorphism and mutation databases Cosmic; 1019928
Cosmic; 2485941