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Cellosaurus SaOS-2 (CVCL_0548)

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Cell line name SaOS-2
Synonyms SAOS-2; Saos-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS
Accession CVCL_0548
Resource Identification Initiative To cite this cell line use: SaOS-2 (RRID:CVCL_0548)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 771.
Doubling time: 48 hours (PubMed=16888811); 45.7 +- 3.3 hours (PubMed=10763916); 35-40 hours (CLS); ~43 hours (DSMZ).
Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for TP53 deletion (PubMed=2823272; PubMed=16888811; PubMed=19787792).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.17
East Asian, North0.46
East Asian, South0.55
South Asian6.66
European, North52.25
European, South38.6
Disease Osteosarcoma (NCIt: C9145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_KU47 (Saos-2 Tet-Off)CVCL_X642 (SAOS-alpha2beta1)CVCL_9563 (SaOS-LM1)
CVCL_9U47 (Saos2-I)CVCL_XF78 (STA5)CVCL_SA52 (SuperSOST C9)
CVCL_SA53 (SuperSOST G5)
Sex of cell Female
Age at sampling 11Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; Cosmic-CLP; DSMZ; ECACC; PubMed=11416159; PubMed=19787792; RCB; TKG

D18S5115 (CCRID; CLS; PubMed=11416159)
15,22 (PubMed=19787792)
Penta D11,12
Penta E14,19

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/Saos-2_cells

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003
Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

Rodan S.B., Imai Y., Thiede M.A., Wesolowski G., Thompson D., Bar-Shavit Z., Shull S., Mann K., Rodan G.A.
Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties.
Cancer Res. 47:4961-4966(1987)

PubMed=2823272; DOI=10.1073/pnas.84.21.7716
Masuda H., Miller C., Koeffler H.P., Battifora H., Cline M.J.
Rearrangement of the p53 gene in human osteogenic sarcomas.
Proc. Natl. Acad. Sci. U.S.A. 84:7716-7719(1987)

PubMed=8617485; DOI=10.1016/S0046-8177(96)90115-X
Scotlandi K., Serra M., Manara M.C., Benini S., Sarti M., Maurici D., Lollini P.-L., Picci P., Bertoni F., Baldini N.
Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.
Hum. Pathol. 27:408-416(1996)

PubMed=10763916; DOI=10.1023/A:1006623001465
Jia S.-F., Worth L.L., Kleinerman E.S.
A nude mouse model of human osteosarcoma lung metastases for evaluating new therapeutic strategies.
Clin. Exp. Metastasis 17:501-506(1999)

PubMed=12645653; DOI=10.1016/S0165-4608(02)00685-4
Ozaki T., Neumann T., Wai D.H., Schaefer K.-L., van Valen F., Lindner N., Scheel C., Bocker W., Winkelmann W., Dockhorn-Dworniczak B., Horst J., Poremba C.
Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping.
Cancer Genet. Cytogenet. 140:145-152(2003)

PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809
Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Cancer Res. 64:3395-3405(2004)

Pautke C., Schieker M., Tischer T., Kolk A., Neth P., Mutschler W., Milz S.
Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.
Anticancer Res. 24:3743-3748(2004)

PubMed=15939397; DOI=10.1016/j.bbrc.2005.05.097
Hausser H.-J., Brenner R.E.
Phenotypic instability of Saos-2 cells in long-term culture.
Biochem. Biophys. Res. Commun. 333:216-222(2005)

PubMed=16888811; DOI=10.1002/jcb.21073
Moneo V., Serelde B.G., Fominaya J., Leal J.F.M., Blanco-Aparicio C., Romero L., Sanchez-Beato M., Cigudosa J.C., Tercero J.C., Piris M.A., Jimeno J., Carnero A.
Extreme sensitivity to Yondelis (Trabectedin, ET-743) in low passaged sarcoma cell lines correlates with mutated p53.
J. Cell. Biochem. 100:339-348(2007)

PubMed=17354236; DOI=10.1002/ijc.22643
Muller C.R., Paulsen E.B., Noordhuis P., Pedeutour F., Saeter G., Myklebost O.
Potential for treatment of liposarcomas with the MDM2 antagonist Nutlin-3A.
Int. J. Cancer 121:199-205(2007)

PubMed=17431109; DOI=10.1158/1535-7163.MCT-06-0729
Moneo V., Serelde B.G., Leal J.F.M., Blanco-Aparicio C., Diaz-Uriarte R., Aracil M., Tercero J.C., Jimeno J., Carnero A.
Levels of p27(kip1) determine Aplidin sensitivity.
Mol. Cancer Ther. 6:1310-1316(2007)

PubMed=19787792; DOI=10.1002/gcc.20717
Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S., Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H., Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W., Buerger H., Aigner T., Gabbert H.E., Poremba C.
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21519327; DOI=10.1038/labinvest.2011.72
Mohseny A.B., Machado I., Cai Y., Schaefer K.-L., Serra M., Hogendoorn P.C.W., Llombart-Bosch A., Cleton-Jansen A.-M.
Functional characterization of osteosarcoma cell lines provides representative models to study the human disease.
Lab. Invest. 91:1195-1205(2011)

PubMed=22075555; DOI=10.1158/0008-5472.CAN-11-2778
Huang G., Yu L., Cooper L.J., Hollomon M., Huls H., Kleinerman E.S.
Genetically modified T cells targeting interleukin-11 receptor alpha-chain kill human osteosarcoma cells and induce the regression of established osteosarcoma lung metastases.
Cancer Res. 72:271-281(2012)

PubMed=23671654; DOI=10.1371/journal.pone.0063056
Lu Y., Soong T.D., Elemento O.
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:E63056-E63056(2013)

PubMed=24758355; DOI=10.1186/1471-2407-14-281
Moneo V., Serelde B.G., Blanco-Aparicio C., Diaz-Uriarte R., Aviles P., Santamaria G., Tercero J.C., Cuevas C., Carnero A.
Levels of active tyrosine kinase receptor determine the tumor response to Zalypsis.
BMC Cancer 14:281-281(2014)

Du L., Fan Q.-M., Tu B., Yan W., Tang T.-T.
Establishment and characterization of a new highly metastatic human osteosarcoma cell line derived from Saos2.
Int. J. Clin. Exp. Pathol. 7:2871-2882(2014)

PubMed=26320182; DOI=10.18632/oncotarget.5177
Ren L., Mendoza A., Zhu J., Briggs J.W., Halsey C., Hong E.S., Burkett S.S., Morrow J., Lizardo M.M., Osborne T., Li S.Q., Luu H.H., Meltzer P., Khanna C.
Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.
Oncotarget 6:29469-29481(2015)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
Teicher B.A., Polley E.C., Kunkel M., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29334376; DOI=10.1038/nm.4475
Morrow J.J., Bayles I., Funnell A.P.W., Miller T.E., Saiakhova A., Lizardo M.M., Bartels C.F., Kapteijn M.Y., Hung S., Mendoza A., Dhillon G., Chee D.R., Myers J.T., Allen F., Gambarotti M., Righi A., DiFeo A., Rubin B.P., Huang A.Y., Meltzer P.S., Helman L.J., Picci P., Versteeg H.H., Stamatoyannopoulos J.A., Khanna C., Scacheri P.C.
Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.
Nat. Med. 24:176-185(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections ATCC; CRL-7939 - Discontinued
BCRJ; 0217
CLS; 300331/p651_SaOS-2
ECACC; 89050205
ICLC; HTL01001
KCB; KCB 200550YJ
KCLB; 30085
KCLB; 80023 - Discontinued
NCBI_Iran; C453
RCB; RCB0428
RCB; RCB3688
TKG; TKG 0469
Cell line databases/resources CLDB; cl4250
CLDB; cl4251
CLDB; cl4252
CLDB; cl5281
CCRID; 3111C0001CCC000025
CCRID; 3131C0001000700114
CCRID; 3142C0001000000074
Cell_Model_Passport; SIDM01084
Cosmic-CLP; 909707
DepMap; ACH-000410
Lonza; 747
TOKU-E; 3085
Ontologies BTO; BTO:0000971
CLO; CLO_0008947
CLO; CLO_0050140
MCCL; MCC:0000449
Biological sample resources BioSample; SAMN01821594
BioSample; SAMN01821661
BioSample; SAMN03471126
BioSample; SAMN10987923
Chemistry resources ChEMBL-Cells; CHEMBL3307737
ChEMBL-Targets; CHEMBL614894
GDSC; 909707
PharmacoDB; Saos2_1343_2019
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM170249
GEO; GSM185150
GEO; GSM185151
GEO; GSM320830
GEO; GSM827588
GEO; GSM879222
GEO; GSM1639703
GEO; GSM1639704
GEO; GSM1670401
GEO; GSM1676314
GEO; GSM1701648
GEO; GSM1915012
GEO; GSM1915013
Other Wikidata; Q7420876
Polymorphism and mutation databases Cosmic; 687930
Cosmic; 755311
Cosmic; 897500
Cosmic; 909707
Cosmic; 931039
Cosmic; 931912
Cosmic; 1044086
Cosmic; 1044269
Cosmic; 1070841
Cosmic; 1074395
Cosmic; 1082492
Cosmic; 1529898
Cosmic; 1733126
Cosmic; 2301582
IARC_TP53; 21653
LiGeA; CCLE_519
Entry history
Entry creation04-Apr-2012
Last entry updated19-Dec-2019
Version number31