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Cellosaurus Sf9 (CVCL_0549)

Cell line name Sf9
Synonyms SF9; sf9; SF-9; Sf-9; sf-9; Sf 9; Spodoptera frugiperda clone 9; Sf clone 9; IPLB-Sf-9AE; IPLB-SF-9AE; IPLB-SF-9; IPLB-Sf-9; IPLB-Sf9
Accession CVCL_0549
Resource Identification Initiative To cite this cell line use: Sf9 (RRID:CVCL_0549)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: Heterogeneous, contains a mixture of diploid and tetraploid cells.
Doubling time: 27 hours (PubMed=21667340); ~50 hours (DSMZ).
Omics: ESTs sequenced.
Omics: Transcriptome analysis.
Omics: Genome sequenced.
Caution: The SfRV rhabdovirus found in Sf9 cells of ATCC CRL-1711 lot 5807852 (PubMed=24672045) does not seem to be expressed in CRL-1711 lot 5814 from 1987 (PubMed=28423032). The SfRV genome is integrated in the Sf9 genome.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Parent: CVCL_0518 (Sf21)
Children:
CVCL_1D71 (Sf-9ET)CVCL_JX36 (Sf9 TiterHigh AC free)CVCL_UF23 (sf9-ht33)
CVCL_UF24 (sf9-ht35)CVCL_Z457 (Sf9-P35AcV5-1)CVCL_Z458 (Sf9-P35AcV5-3)
CVCL_4U10 (Sf900+)CVCL_Z459 (Sf9S)CVCL_Z364 (Sfbeta4GalT)
CVCL_Z454 (VE-CL-01)CVCL_Z455 (VE-CL-02)CVCL_Z456 (VE-CL-03)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Sf9_(cells)
https://www.thermofisher.com/order/catalog/product/B82501
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-311.html
http://www.ars.usda.gov/sp2userfiles/person/3489/sf9.pdf
https://oetltd.com/product/sf9-cells/
http://www.the-scientist.com/?articles.view/articleNo/40052/title/Virus-Found-in-Sf9-Cell-Line/
Publications

PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=12052082; DOI=10.1021/bp020028
Jarman-Smith R.F., Armstrong S.J., Mannix C.J., Al-Rubeai M.
Chromosome instability in Spodoptera frugiperda Sf-9 cell line.
Biotechnol. Prog. 18:623-628(2002)

PubMed=14668217; DOI=10.1093/bioinformatics/btg324
Landais I., Ogliastro M., Mita K., Nohata J., Lopez-Ferber M., Duonor-Cerutti M., Shimada T., Fournier P., Devauchelle G.
Annotation pattern of ESTs from Spodoptera frugiperda Sf9 cells and analysis of the ribosomal protein genes reveal insect-specific features and unexpectedly low codon usage bias.
Bioinformatics 19:2343-2350(2003)

PubMed=19003226; DOI=10.1023/B:CYTO.0000043394.40309.48
Jarman-Smith R.F., Mannix C.J., Al-Rubeai M.
Characterisation of tetraploid and diploid clones of Spodoptera frugiperda cell line.
Cytotechnology 44:15-25(2004)

PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)

DOI=10.3923/jas.2007.4040.4043
Sadeq K.N.A., Yao H.-C., Peng J.-X.
Identification of insect cell lines from 8 lepidopteran species by DNA amplification fingerprinting.
J. Appl. Sci. 7:4040-4044(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20300881; DOI=10.1007/s12033-010-9268-3
Krammer F., Schinko T., Palmberger D., Tauer C., Messner P., Grabherr R.
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)

PubMed=21667340; DOI=10.1007/s12250-011-3177-x
Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)

PubMed=23891577; DOI=10.1016/j.tiv.2013.07.007
Curtis T.M., Collins A.M., Gerlach B.D., Brennan L.M., Widder M.W., van der Schalie W.H., Vo N.T.K., Bols N.C.
Suitability of invertebrate and vertebrate cells in a portable impedance-based toxicity sensor: temperature mediated impacts on long-term survival.
Toxicol. In Vitro 27:2061-2066(2013)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)

PubMed=28423032; DOI=10.1371/journal.pone.0175633
Hashimoto Y., Macri D., Srivastava I., McPherson C., Felberbaum R., Post P., Cox M.
Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line.
PLoS ONE 12:E0175633-E0175633(2017)

PubMed=28839023; DOI=10.1128/genomeA.00829-17
Nandakumar S., Ma H., Khan A.S.
Whole-genome sequence of the Spodoptera frugiperda Sf9 insect cell line.
Genome Announc. 5:e00829.17-e00829.17(2017)

PubMed=29038528; DOI=10.1038/s41598-017-12713-9
Shu B.-S., Zhang J.-J., Sethuraman V., Cui G.-F., Yi X., Zhong G.-H.
Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin.
Sci. Rep. 7:13231-13231(2017)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

Cross-references
Cell line collections ATCC; CRL-1711
BCRC; 60011
CCLV; CCLV-RIE 0352
CLS; 604328/p700_Sf9
DSMZ; ACC-125
ECACC; 89070101
IZSLER; BS CL 157
KCB; KCB 200807YJ
NCBI_Iran; C425
NISES; SK0002
RCB; RCB0563 - Discontinued
Cell line databases/resources CLDB; cl4277
CLDB; cl4278
CLDB; cl4279
Lonza; 1014
TOKU-E; 3107
Ontologies BTO; BTO:0001240
CLO; CLO_0008988
CLO; CLO_0008989
CLO; CLO_0008990
MCCL; MCC:0000450
MeSH; D061987
Chemistry resources ChEMBL-Cells; CHEMBL3308860
Other Wikidata; Q7459700