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Cellosaurus T24 (CVCL_0554)

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Cell line name T24
Synonyms T-24; T 24
Accession CVCL_0554
Resource Identification Initiative To cite this cell line use: T24 (RRID:CVCL_0554)
Comments Part of: BLA-40 bladder carcinoma cell line panel.
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Part of: UBC-40 urothelial bladder cancer cell line index.
Doubling time: 19 hours (PubMed=4133950); 21 hours (PubMed=3708594); 23.2 hours (PubMed=26055179); ~48 hours (DSMZ).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Exosome proteome analysis.
Omics: GPI-anchored proteins analysis by proteomics.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for HRAS p.Gly12Val (c.35G>T) (ATCC).
TERT c.228C>T (-124C>T); in promoter (PubMed=24035680).
Homozygous for TP53 p.Tyr126Ter (c.378C>G) (ATCC; PubMed=7787250).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_6876 (ACCS)CVCL_2029 (ECV-304)CVCL_2893 (EJ-1)
CVCL_2443 (Ej138)CVCL_8199 (GHE)CVCL_8223 (hAG)
CVCL_AT91 (HBMEC-2)CVCL_8229 (HCV-29TMV)CVCL_8238 (Hu456)
CVCL_8239 (Hu549)CVCL_8242 (Hu609Tmv)CVCL_8243 (Hu961a)
CVCL_4015 (JCA-1)CVCL_F660 (KS Y-1)CVCL_9826 (MGH-U2)
CVCL_8271 (RAMAK-1)CVCL_2728 (T24/83)CVCL_Y181 (T24/DDP10)
CVCL_Y182 (T24/DDP5)CVCL_Y183 (T24/DDP7)CVCL_VL60 (T24M)
CVCL_RR21 (T24rCDDP1000)CVCL_RR22 (T24rGEMCI20)CVCL_RR23 (T24rVINB20)
CVCL_RS42 (T24rYM155_20nM)CVCL_M892 (T24T)CVCL_4014 (TSU-Pr1)
CVCL_8155 (UM-UC-2)CVCL_M721 (UROtsa/F35)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; DSMZ; JCRB; KCLB; PubMed=11416159; PubMed=27270441; RCB; TKG

CSF1PO10,12 (ATCC; CLS; Cosmic-CLP; DSMZ; JCRB; KCLB; PubMed=11416159; PubMed=27270441; TKG)
12 (RCB)
D3S135816,21 (CLS)
16 (KCLB)
D5S81810,12 (ATCC; CLS; Cosmic-CLP; DSMZ; JCRB; KCLB; PubMed=11416159; PubMed=27270441; TKG)
10 (RCB)
D8S11799,14 (CLS)
14 (PubMed=11416159)
D13S31712 (ATCC; CLS; Cosmic-CLP; JCRB; KCLB; PubMed=27270441; RCB; TKG)
9,12 (DSMZ)
Penta D11,15
Penta E7,10
vWA17 (ATCC; Cosmic-CLP; JCRB; RCB; TKG)
17,19 (CLS; DSMZ; KCLB; PubMed=11416159; PubMed=27270441)

Run an STR similarity search on this cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=T24

PubMed=4133950; DOI=10.1002/ijc.2910110327
Bubenik J., Baresova M., Viklicky V., Jakoubkova J., Sainerova H., Donner J.
Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen.
Int. J. Cancer 11:765-773(1973)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=864752; DOI=10.1093/jnci/58.6.1743
Marshall C.J., Franks L.M., Carbonell A.W.
Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.
J. Natl. Cancer Inst. 58:1743-1751(1977)

PubMed=870558; DOI=10.1177/25.4.870558
Benham F.J., Cottell D.C., Franks L.M., Wilson P.D.
Alkaline phosphatase activity in human bladder tumor cell lines.
J. Histochem. Cytochem. 25:266-274(1977)

Fogh J.
Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.
Natl. Cancer Inst. Monogr. 49:5-9(1978)

PubMed=663932; DOI=10.1620/tjem.124.33
Kato T., Ishikawa K., Nemoto R., Senoo A., Amano Y.
Morphological characterization of two established cell lines, T24 and MGH-U1, derived from human urinary bladder carcinoma.
Tohoku J. Exp. Med. 124:339-349(1978)

Williams R.D.
Human urologic cancer cell lines.
Invest. Urol. 17:359-363(1980)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003
Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=7185004; DOI=10.2302/kjm.31.127
Tachibana M.
Studies on cellular adhesiveness in five different culture cell lines derived from carcinoma of the urinary bladder.
Keio J. Med. 31:127-148(1982)

Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=6823318; DOI=10.1038/301429a0
O'Toole C.M., Povey S., Hepburn P.J., Franks L.M.
Identity of some human bladder cancer cell lines.
Nature 301:429-430(1983)

PubMed=6826254; DOI=10.1002/ijc.2910310308
Paulie S., Hansson Y., Lundblad M.L., Perlmann P.
Lectins as probes for identification of tumor-associated antigens on urothelial and colonic carcinoma cell lines.
Int. J. Cancer 31:297-303(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

Masters J.R.W., Hepburn P.J., Walker L., Highman W.J., Trejdosiewicz L.K., Povey S., Parkar M., Hill B.T., Riddle P.R., Franks L.M.
Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.
Cancer Res. 46:3630-3636(1986)

PubMed=2607719; DOI=10.5980/jpnjurol1989.80.988
Kihara K., Kageyama Y., Sumi S., Higashi Y., Fukui I., Oshima H.
A study of intercellular communication of human transitional cell carcinoma cell lines.
Nihon Hinyokika Gakkai Zasshi 80:988-994(1989)

Cooper M.J., Haluschak J.J., Johnson D., Schwartz S., Morrison L.J., Lippa M., Hatzivassiliou G., Tan J.
p53 mutations in bladder carcinoma cell lines.
Oncol. Res. 6:569-579(1994)

PubMed=8873383; DOI=10.1007/BF00295899
Stadler W.M., Olopade O.I.
The 9p21 region in bladder cancer cell lines: large homozygous deletion inactivate the CDKN2, CDKN2B and MTAP genes.
Urol. Res. 24:239-244(1996)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

Markl I.D.C., Jones P.A.
Presence and location of TP53 mutation determines pattern of CDKN2A/ARF pathway inactivation in bladder cancer.
Cancer Res. 58:5348-5353(1998)

Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T., Mizusawa H.
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=11921286; DOI=10.1002/gcc.10050
Williams S.V., Sibley K.D., Davies A.M., Nishiyama H., Hornigold N., Coulter J., Kennedy W.J., Skilleter A., Habuchi T., Knowles M.A.
Molecular genetic analysis of chromosome 9 candidate tumor-suppressor loci in bladder cancer cell lines.
Genes Chromosomes Cancer 34:86-96(2002)

PubMed=15846775; DOI=10.1002/gcc.20166
Williams S.V., Adams J., Coulter J., Summersgill B.M., Shipley J.M., Knowles M.A.
Assessment by M-FISH of karyotypic complexity and cytogenetic evolution in bladder cancer in vitro.
Genes Chromosomes Cancer 43:315-328(2005)

PubMed=16885334; DOI=10.1158/0008-5472.CAN-06-1182
Lopez-Knowles E., Hernandez S., Malats N., Kogevinas M., Lloreta J., Carrato A., Tardon A., Serra C., Real F.X.
PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors.
Cancer Res. 66:7401-7404(2006)

PubMed=19105184; DOI=10.1002/pmic.200800121
Makridakis M., Gagos S., Petrolekas A., Roubelakis M.G., Bitsika V., Stravodimos K., Pavlakis K., Anagnou N.P., Coleman J., Vlahou A.
Chromosomal and proteome analysis of a new T24-based cell line model for aggressive bladder cancer.
Proteomics 9:287-298(2009)

PubMed=19375735; DOI=10.1016/j.juro.2009.01.108
Chiong E., Dadbin A., Harris L.D., Sabichi A.L., Grossman H.B.
The use of short tandem repeat profiling to characterize human bladder cancer cell lines.
J. Urol. 181:2737-2748(2009)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23401075; DOI=10.1002/path.4176
Guo Y., Chekaluk Y., Zhang J., Du J., Gray N.S., Wu C.-L., Kwiatkowski D.J.
TSC1 involvement in bladder cancer: diverse effects and therapeutic implications.
J. Pathol. 230:17-27(2013)

PubMed=24367658; DOI=10.1371/journal.pone.0084411
Ross R.L., Burns J.E., Taylor C.F., Mellor P., Anderson D.H., Knowles M.A.
Identification of mutations in distinct regions of p85 alpha in urothelial cancer.
PLoS ONE 8:E84411-E84411(2013)

PubMed=24018021; DOI=10.1016/j.eururo.2013.08.052
Allory Y., Beukers W., Sagrera A., Flandez M., Marques M., Marquez M., van der Keur K.A., Dyrskjot L., Lurkin I., Vermeij M., Carrato A., Lloreta J., Lorente J.A., Carrillo-de-Santa-Pau E., Masius R.G., Kogevinas M., Steyerberg E.W., van Tilborg A.A.G., Abas C., Orntoft T.F., Zuiverloon T.C.M., Malats N., Zwarthoff E.C., Real F.X.
Telomerase reverse transcriptase promoter mutations in bladder cancer: high frequency across stages, detection in urine, and lack of association with outcome.
Eur. Urol. 65:360-366(2014)

PubMed=24035680; DOI=10.1016/j.eururo.2013.08.057
Hurst C.D., Platt F.M., Knowles M.A.
Comprehensive mutation analysis of the TERT promoter in bladder cancer and detection of mutations in voided urine.
Eur. Urol. 65:367-369(2014)

PubMed=24376083; DOI=10.1002/pmic.201300452
Jeppesen D.K., Nawrocki A., Jensen S.G., Thorsen K., Whitehead B., Howard K.A., Dyrskjot L., Orntoft T.F., Larsen M.R., Ostenfeld M.S.
Quantitative proteomics of fractionated membrane and lumen exosome proteins from isogenic metastatic and nonmetastatic bladder cancer cells reveal differential expression of EMT factors.
Proteomics 14:699-712(2014)

PubMed=24459064; DOI=10.1007/s13277-013-1604-3
Pinto-Leite R., Carreira I., Melo J., Ferreira S.I., Ribeiro I., Ferreira J., Filipe M., Bernardo C., Arantes-Rodrigues R., Oliveira P., Santos L.
Genomic characterization of three urinary bladder cancer cell lines: understanding genomic types of urinary bladder cancer.
Tumor Biol. 35:4599-4617(2014)

PubMed=25997541; DOI=10.1186/s12864-015-1450-3
Earl J., Rico D., Carrillo-de-Santa-Pau E., Rodriguez-Santiago B., Mendez-Pertuz M., Auer H., Gomez G., Grossman H.B., Pisano D.G., Schulz W.A., Perez-Jurado L.A., Carrato A., Theodorescu D., Chanock S.J., Valencia A., Real F.X.
The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies.
BMC Genomics 16:403-403(2015)

PubMed=26055179; DOI=10.1016/j.tranon.2015.04.002
Vallo S., Michaelis M., Rothweiler F., Bartsch G., Gust K.M., Limbart D.M., Rodel F., Wezel F., Haferkamp A., Cinatl J. Jr.
Drug-resistant urothelial cancer cell lines display diverse sensitivity profiles to potential second-line therapeutics.
Transl. Oncol. 8:210-216(2015)

PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)

PubMed=27141528; DOI=10.1016/j.dib.2016.04.001
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27270441; DOI=10.1038/onc.2016.172
Nickerson M.L., Witte N., Im K.M., Turan S., Owens C., Misner K., Tsang S.X., Cai Z., Wu S., Dean M., Costello J.C., Theodorescu D.
Molecular analysis of urothelial cancer cell lines for modeling tumor biology and drug response.
Oncogene 36:35-46(2017)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29732388; DOI=10.3233/BLC-180167
Zuiverloon T.C.M., de Jong F.C., Costello J.C., Theodorescu D.
Systematic review: characteristics and preclinical uses of bladder cancer cell lines.
Bladder Cancer 4:169-183(2018)

Cell line collections ATCC; HTB-4
BCRC; 60062
BCRJ; 0231
CLS; 300352/p619_T24
KCB; KCB 92027YJ
KCLB; 30004
RCB; RCB0431
RCB; RCB2536
TKG; TKG 0443
Cell line databases/resources CLDB; cl4452
CCRID; 3111C0001CCC000295
CCRID; 3131C0001000700055
CCRID; 3142C0001000000076
Cell_Model_Passport; SIDM01184
Cosmic-CLP; 724812
GDSC; 724812
LINCS_HMS; 50048
Lonza; 862
TOKU-E; 3252
Ontologies BTO; BTO:0001345
CLO; CLO_0009235
CLO; CLO_0009245
CLO; CLO_0050843
CLO; CLO_0050844
EFO; EFO_0002864
MCCL; MCC:0000455
Biological sample resources BioSample; SAMN03472744
BioSample; SAMN03473324
Chemistry resources ChEMBL-Cells; CHEMBL3307700
ChEMBL-Targets; CHEMBL614774
Gene expression databases ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM136233
GEO; GSM492493
GEO; GSM887683
GEO; GSM888775
GEO; GSM1374940
GEO; GSM1574571
GEO; GSM1670520
Other Wikidata; Q54971454
Polymorphism and mutation databases Cosmic; 716174
Cosmic; 724812
Cosmic; 755400
Cosmic; 845561
Cosmic; 846275
Cosmic; 925832
Cosmic; 928817
Cosmic; 943742
Cosmic; 1001664
Cosmic; 1016880
Cosmic; 1046673
Cosmic; 1285130
Cosmic; 1285985
Cosmic; 1302355
Cosmic; 1927302
Cosmic; 1938698
Cosmic; 2037960
Cosmic; 2050465
Cosmic; 2057459
Cosmic; 2444230
Cosmic; 2686118
Cosmic; 2700999
IARC_TP53; 3722
IARC_TP53; 21131
IARC_TP53; 30178
Proteomic databases PRIDE; PXD003105