Cellosaurus T84 (CVCL_0555)

Cell line name T84
Synonyms T-84; T 84
Accession CVCL_0555
Resource Identification Initiative To cite this cell line use: T84 (RRID:CVCL_0555)
Comments Part of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Microsatellite instability: Stable (MSS) (PubMed=25926053; Sanger).
Sequence variation: APC p.Leu1488fs*19 (PubMed=24755471).
Sequence variation: KRAS p.Gly13Asp (PubMed=24755471).
Sequence variation: PIK3CA p.Glu542Lys (PubMed=24755471).
Sequence variation: SMAD4 p.Lys340Asn (PubMed=24755471).
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: N-glycan profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Colon adenocarcinoma (NCIt: C4349)
Derived from metastatic site: Lung.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10
D13S3179
D16S53910,11
D18S5117
D21S1131
D3S135819
D5S81812
D7S8208,10
D8S117915
FGA24
Penta D9
Penta E14
TH016,9
TPOX8,11 (AddexBio; ATCC; Cosmic-CLP)
8 (CLS; ECACC; PubMed=25877200)
vWA17,18
Publications

PubMed=2110452; DOI=10.1042/bj2670491
McCool D.J., Marcon M.A., Forstner J.F., Forstner G.G.
The T84 human colonic adenocarcinoma cell line produces mucin in culture and releases it in response to various secretagogues.
Biochem. J. 267:491-500(1990)

PubMed=10612807; DOI=10.1002/(SICI)1098-2264(200002)27:2<183::AID-GCC10>3.0.CO;2-P
Ghadimi B.M., Sackett D.L., Difilippantonio M.J., Schrock E., Neumann T., Jauho A., Auer G., Ried T.
Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.
Genes Chromosomes Cancer 27:183-190(2000)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=19927377; DOI=10.1002/gcc.20730
Knutsen T., Padilla-Nash H.M., Wangsa D., Barenboim-Stapleton L., Camps J., McNeil N., Difilippantonio M.J., Ried T.
Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.
Genes Chromosomes Cancer 49:204-223(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23272949; DOI=10.1186/1755-8794-5-66
Schlicker A., Beran G., Chresta C.M., McWalter G., Pritchard A., Weston S., Runswick S., Davenport S., Heathcote K., Castro D.A., Orphanides G., French T., Wessels L.F.A.
Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.
BMC Med. Genomics 5:66-66(2012)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

DOI=10.1101/092767
Roumeliotis T.I., Williams S.P., Goncalves E., Zamanzad G.F., Aben N., Michaut M., Schubert M., Wright J.C., Yang M., Alsinet C., Dienstmann R., Guinney J., Beltrao P., Brazma A., Stegle O., Adams D.J., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.
Genomic determinants of protein abundance variation in colorectal cancer cells.
bioRxiv 2016:092767-092767(2016)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

Cross-references
Cell line collections AddexBio; C0009008/70
ATCC; CCL-248
BCRC; 60149
BCRJ; 0232
CLS; 300354/p479_T84
ECACC; 88021101
ICLC; HTL95022
IZSLER; BS TCL 154
Cell line databases/resources CLDB; cl4474
CLDB; cl4475
CCLE; T84_LARGE_INTESTINE
CCRID; 3111C0001CCC000056
CCRID; 3131C0001001200009
ColonAtlas; T84
Cosmic-CLP; 909761
GDSC; 909761
IGRhCellID; T84
LINCS_LDP; LCL-1166
SKY/M-FISH/CGH; 2853
Ontologies BTO; BTO:0002032
CLO; CLO_0009254
EFO; EFO_0002084
MCCL; MCC:0000456
Biological sample resources BioSample; SAMN03471378
BioSample; SAMN03473196
Chemistry resources ChEMBL-Cells; CHEMBL3307275
ChEMBL-Targets; CHEMBL613531
Gene expression databases GEO; GSM206556
GEO; GSM741255
GEO; GSM827303
GEO; GSM887686
GEO; GSM888778
GEO; GSM1346895
GEO; GSM1374953
GEO; GSM1448090
GEO; GSM1670522
Metabolomic databases MetaboLights; MTBLS227
Polymorphism and mutation databases Cosmic; 887245
Cosmic; 889532
Cosmic; 909761
Cosmic; 983742
Cosmic; 995406
Cosmic; 1043832
Cosmic; 1122335
Cosmic; 1184103
Cosmic; 1187325
Cosmic; 1479590
Cosmic; 1537487
Cosmic; 1609494
Cosmic; 1676743
Cosmic; 1708411
Cosmic; 1803953
Cosmic; 1888953
Cosmic; 2301548
Cosmic; 2302022