Cellosaurus cell line TK6 (CVCL_0561)

• Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  
• Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=7859358).
  
• Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=7744731; PubMed=11221843).
  

PubMed=214074; DOI=10.1016/0006-291X(78)90185-7
Skopek T.R., Liber H.L., Penman B.W., Thilly W.G.
Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay.
Biochem. Biophys. Res. Commun. 84:411-416(1978)

Patent=US4302535
Skopek T.R., Liber H.L., Penman B.W., Thilly W.G., Hoppe H. IV
Assay for mutagenesis in heterozygous diploid human lymphoblasts.
Patent number US4302535, 24-Nov-1981

PubMed=3012798; DOI=10.1007/BF01570784
Yandell D.W., Dryja T.P., Little J.B.
Somatic mutations at a heterozygous autosomal locus in human cells occur more frequently by allele loss than by intragenic structural alterations.
Somat. Cell Mol. Genet. 12:255-263(1986)

PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7565900; DOI=10.1016/0165-7992(95)00027-5
Zhang L.-S., Honma M., Hayashi M., Suzuki T., Matsuoka A., Sofuni T.
A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test.
Mutat. Res. 347:105-115(1995)

PubMed=7744731; DOI=10.1074/jbc.270.19.11033
Little J.B., Nagasawa H., Keng P.C., Yu Y.-J., Li C.-Y.
Absence of radiation-induced G1 arrest in two closely related human lymphoblast cell lines that differ in p53 status.
J. Biol. Chem. 270:11033-11036(1995)

PubMed=7859358; DOI=10.1093/carcin/16.2.267
Giver C.R., Nelson S.L. Jr., Cha M.Y., Pongsaensook P., Grosovsky A.J.
Mutational spectrum of X-ray induced TK- human cell mutants.
Carcinogenesis 16:267-275(1995)

PubMed=9258685; DOI=10.1093/genetics/146.4.1429
Giver C.R., Grosovsky A.J.
Single and coincident intragenic mutations attributable to gene conversion in a human cell line.
Genetics 146:1429-1439(1997)

PubMed=10397247
Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.
Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.
Cancer Res. 59:3073-3076(1999)

PubMed=11221843
Wiese C., Gauny S.S., Liu W.-C., Cherbonnel-Lasserre C.L., Kronenberg A.
Different mechanisms of radiation-induced loss of heterozygosity in two human lymphoid cell lines from a single donor.
Cancer Res. 61:1129-1137(2001)

PubMed=15033590; DOI=10.1289/ehp.6709
Newton R.K., Aardema M.J., Aubrecht J.
The utility of DNA microarrays for characterizing genotoxicity.
Environ. Health Perspect. 112:420-422(2004)

PubMed=22042714; DOI=10.1093/mutage/ger074
Hashimoto K., Nakajima Y., Uematsu R., Chatani F.
Difference in susceptibility to morphological changes in the nucleus to aneugens between p53-competent and p53-abrogated lymphoblastoid cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.
Mutagenesis 27:287-293(2012)

PubMed=22525470; DOI=10.18926/AMO/48262
Oka H., Ouchida M., Kondo T., Morita F., Shimizu K.
Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation.
Acta Med. Okayama 66:119-129(2012)

PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006
Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B., Dobrovolsky V.N.
Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.
Mutat. Res. 795:60-69(2016)

PubMed=27692294; DOI=10.1016/j.mrgentox.2016.08.001
Lorge E., Moore M.M., Clements J., O'Donovan M., Fellows M.D., Honma M., Kohara A., Galloway S., Armstrong M.J., Thybaud V., Gollapudi B., Aardema M.J., Tanir J.Y.
Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing.
Mutat. Res. 809:1-15(2016)