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Cellosaurus TK6 (CVCL_0561)

[Text version]

Cell line name TK6
Synonyms TK-6; H2BT
Accession CVCL_0561
Resource Identification Initiative To cite this cell line use: TK6 (RRID:CVCL_0561)
Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8015.
Population: Caucasian.
Doubling time: 12.2 hours (PubMed=7744731).
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Omics: Deep RNAseq analysis.
Omics: Transcriptome analysis.
Derived from sampling site: Spleen.
Sequence variations
  • Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=7859358).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=7744731; PubMed=11221843).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_X906 (HH4)
Children:
CVCL_A1RF (NH32 )CVCL_AR68 (TK6 (IVGT) )CVCL_2733 (TK6TGR )
CVCL_WN50 (TSCE5 )CVCL_A1SH (TX545 )
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
STR profile Source(s): ATCC; CLS; ECACC; JCRB

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135816
D5S81812,13
D7S8209,11
D8S117910,13
D13S31711
D16S53911,12
D18S5111,16
D21S1129
FGA22,24
Penta D11,12
Penta E5,7
TH018,9.3
TPOX8,11
vWA17,20

Run an STR similarity search on this cell line
Publications

PubMed=214074; DOI=10.1016/0006-291X(78)90185-7
Skopek T.R., Liber H.L., Penman B.W., Thilly W.G.
Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay.
Biochem. Biophys. Res. Commun. 84:411-416(1978)

Patent=US4302535
Skopek T.R., Liber H.L., Penman B.W., Thilly W.G., Hoppe H. IV
Assay for mutagenesis in heterozygous diploid human lymphoblasts.
Patent number US4302535, 24-Nov-1981

PubMed=3012798; DOI=10.1007/BF01570784
Yandell D.W., Dryja T.P., Little J.B.
Somatic mutations at a heterozygous autosomal locus in human cells occur more frequently by allele loss than by intragenic structural alterations.
Somat. Cell Mol. Genet. 12:255-263(1986)

PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q., Mencl J., Olive P.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7565900; DOI=10.1016/0165-7992(95)00027-5
Zhang L.-S., Honma M., Hayashi M., Suzuki T., Matsuoka A., Sofuni T.
A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test.
Mutat. Res. 347:105-115(1995)

PubMed=7744731; DOI=10.1074/jbc.270.19.11033
Little J.B., Nagasawa H., Keng P.C., Yu Y., Li C.-Y.
Absence of radiation-induced G1 arrest in two closely related human lymphoblast cell lines that differ in p53 status.
J. Biol. Chem. 270:11033-11036(1995)

PubMed=7859358; DOI=10.1093/carcin/16.2.267
Giver C.R., Nelson S.L. Jr., Cha M.Y., Pongsaensook P., Grosovsky A.J.
Mutational spectrum of X-ray induced TK- human cell mutants.
Carcinogenesis 16:267-275(1995)

PubMed=9258685
Giver C.R., Grosovsky A.J.
Single and coincident intragenic mutations attributable to gene conversion in a human cell line.
Genetics 146:1429-1439(1997)

PubMed=10397247
Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.
Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.
Cancer Res. 59:3073-3076(1999)

PubMed=11221843
Wiese C., Gauny S.S., Liu W.-C., Cherbonnel-Lasserre C.L., Kronenberg A.
Different mechanisms of radiation-induced loss of heterozygosity in two human lymphoid cell lines from a single donor.
Cancer Res. 61:1129-1137(2001)

PubMed=15033590; DOI=10.1289/ehp.6709
Newton R.K., Aardema M.J., Aubrecht J.
The utility of DNA microarrays for characterizing genotoxicity.
Environ. Health Perspect. 112:420-422(2004)

PubMed=22042714; DOI=10.1093/mutage/ger074
Hashimoto K., Nakajima Y., Uematsu R., Chatani F.
Difference in susceptibility to morphological changes in the nucleus to aneugens between p53-competent and p53-abrogated lymphoblastoid cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.
Mutagenesis 27:287-293(2012)

PubMed=22525470; DOI=10.18926/AMO/48262
Oka H., Ouchida M., Kondo T., Morita F., Shimizu K.
Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation.
Acta Med. Okayama 66:119-129(2012)

PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006
Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B., Dobrovolsky V.N.
Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.
Mutat. Res. 795:60-69(2016)

PubMed=27692294; DOI=10.1016/j.mrgentox.2016.08.001
Lorge E., Moore M.M., Clements J., O'Donovan M., Fellows M.D., Honma M., Kohara A., Galloway S., Armstrong M.J., Thybaud V., Gollapudi B., Aardema M.J., Tanir J.Y.
Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing.
Mutat. Res. 809:1-15(2016)

Cross-references
Cell line collections ATCC; CRL-8015
BCRC; 60107
CLS; 300357
ECACC; 95111735
Cell line databases/resources CLO; CLO_0009357
MCCL; MCC:0000463
CLDB; cl4519
Anatomy/cell type resources BTO; BTO:0003707
Biological sample resources BioSample; SAMN03471129
Encyclopedic resources Wikidata; Q54972535
Gene expression databases ArrayExpress; E-TOXM-7
GEO; GSE65477
Polymorphism and mutation databases IARC_TP53; 21695
Entry history
Entry creation04-Apr-2012
Last entry update23-Jun-2022
Version number22