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Cellosaurus NIH 3T3 (CVCL_0594)

Cell line name NIH 3T3
Synonyms NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3
Accession CVCL_0594
Resource Identification Initiative To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)
Comments Part of: ENCODE project mouse cell lines.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: ~20 hours (DSMZ).
Omics: SNP array analysis.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Children:
CVCL_M056 (3J1)CVCL_1H10 (3T3-msCD40L)CVCL_1E11 (3T3.T4)
CVCL_F962 (A4 [Mouse])CVCL_4104 (Ampli-GPE)CVCL_9242 (ANN-1)
CVCL_0396 (B104-1-1)CVCL_2402 (C1I)CVCL_KS69 (CellSensor dhfr(E2F)-bla NIH3T3)
CVCL_KS54 (CellSensor Gli-bla NIH3T3)CVCL_F498 (Cle-H3)CVCL_B952 (D4)
CVCL_2437 (Df)CVCL_6254 (DHFR-G8)CVCL_2441 (E111)
CVCL_6D48 (EGFR-T17)CVCL_5741 (EJ-6-2-Bam-6a)CVCL_U422 (Flp-In-3T3)
CVCL_8956 (GP+E-86)CVCL_8957 (GP+envAM-12)CVCL_D576 (HER14)
CVCL_4347 (II b)CVCL_6662 (K:Molv NIH/3T3)CVCL_KS91 (MEF/3T3 Tet-Off)
CVCL_U510 (MG87)CVCL_Y512 (MOP-6)CVCL_2617 (MOP-8)
CVCL_D900 (NEF)CVCL_5I45 (NIH 2/4)CVCL_QY95 (NIH 3T3 Atg5 KO)
CVCL_HC50 (NIH 3T3 Duo Parental)CVCL_L994 (NIH 3T3 p-7 cl-3)CVCL_D675 (NIH 3T3-IL-7)
CVCL_5845 (NIH 3T3-ras)CVCL_9116 (NIH 3T3-TK-)CVCL_0R23 (NIH 3T3/DC-SIGN)
CVCL_LG16 (NIH 3T3/HER2-3(400))CVCL_0R24 (NIH 3T3/L-SIGN)CVCL_7219 (NIH-3T3 4-2)
CVCL_7220 (NIH-3T3 clone 5611)CVCL_L990 (NIH-3T3-A14)CVCL_L991 (NIH-3T3-G185)
CVCL_7218 (NIH-3T3D4)CVCL_V360 (NIH/3T3 Tet-On 3G)CVCL_L992 (NIH3T3-3)
CVCL_VA35 (NIH3T3-47)CVCL_M025 (NIH3T3/13C7)CVCL_L995 (NIH3T3/14-1)
CVCL_4Y01 (NIH3T3/ATCC/CMV-Luc)CVCL_JY97 (NIH3T3/GFP)CVCL_KS45 (NIH3T3/NFkB-luc)
CVCL_D206 (NWT-21)CVCL_2151 (OMEGA-E)CVCL_2165 (Psi-2)
CVCL_W642 (Psi-AM22b)CVCL_4278 (Psi-CRE)CVCL_4279 (Psi-CRIP)
CVCL_LK41 (RL7)CVCL_9Y75 (Sb51)CVCL_2721 (Shh Light II)
CVCL_0552 (Swiss-LAPA)CVCL_3433 (WOP)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/3T3_cells
http://nih3t3.com/
http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
https://www.atcc.org/support/faqs/89140/ATCC%20CCL92%20vs%20ATCC%20CRL1658-453.aspx
http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
http://www.proteinatlas.org/learn/cellines
Publications

PubMed=2463382
Raptis L., Bolen J.B.
Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
J. Virol. 63:753-758(1989)

PubMed=9753870
Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S., Moni M.S., Rajkumar T., Deshmukh S.
Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Indian J. Biochem. Biophys. 35:108-114(1998)

PubMed=23321776; DOI=10.1369/0022155413476868
Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A., Liehr T.
First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847-847(2014)

Cross-references
Cell line collections AddexBio; P0011001/83
ATCC; CRL-1658
ATCC; CRL-6442 - Discontinued
BCRC; 60008
BCRJ; 0191
CLS; 400101/p677_NIH-3T3
DSMZ; ACC-59
ECACC; 93061524
ICLC; AL07001
IZSLER; BS CL 165
KCB; KCB 200645YJ
KCLB; 21658
NCBI_Iran; C156
NIH-ARP; 9946-386
RCB; RCB2767
TKG; TKG 0299
Cell line databases/resources CLDB; cl3702
CLDB; cl3704
CLDB; cl3705
CLDB; cl3706
CLDB; cl3707
CLDB; cl3708
CLDB; cl3709
CLDB; cl3710
CLDB; cl3711
CLDB; cl4451
CLDB; cl7203
CCRID; 3111C0001CCC000018
CCRID; 3111C0002000000034
CCRID; 3131C0001000300006
CCRID; 3142C0001000000030
Lonza; 115
TOKU-E; 2753
TOKU-E; 3547
Ontologies BTO; BTO:0000944
BTO; BTO:0005065
CLO; CLO_0008172
CLO; CLO_0009227
CLO; CLO_0050548
EFO; EFO_0001222
MCCL; MCC:0000362
MeSH; D041681
Biological sample resources eagle-i; dartmouth.eagle-i.net/i/0000013e-d70e-ad79-6a92-b95280000000
eagle-i; hawaii.eagle-i.net/i/0000012b-5666-eb66-2f73-b43980000000
ENCODE; ENCBS117ENC
ENCODE; ENCBS990YBC
Chemistry resources ChEMBL-Cells; CHEMBL3307716
ChEMBL-Targets; CHEMBL614822
Gene expression databases GEO; GSM1014177
Other Wikidata; Q4636420
Proteomic databases PRIDE; PXD001241
PRIDE; PXD004929