Cellosaurus cell line NIH 3T3 (CVCL

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Cellosaurus NIH 3T3 (CVCL_0594)

[Text version]

Cell line name

NIH 3T3

Synonyms

NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Accession

CVCL_0594

Resource Identification Initiative

To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)

Comments

Part of: ENCODE project mouse cell lines.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: ~20 hours (DSMZ).
Omics: SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida J.L.).
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida J.L.).

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.

Hierarchy

Children:

CVCL_M056 (3J1)	CVCL_1H10 (3T3-msCD40L)	CVCL_1E11 (3T3.T4)
CVCL_F962 (A4 [Mouse])	CVCL_4104 (Ampli-GPE)	CVCL_9242 (ANN-1)
CVCL_0396 (B104-1-1)	CVCL_2402 (C1I)	CVCL_KS69 (CellSensor dhfr(E2F)-bla NIH3T3)
CVCL_KS54 (CellSensor Gli-bla NIH3T3)	CVCL_F498 (Cle-H3)	CVCL_B952 (D4)
CVCL_2437 (Df)	CVCL_6254 (DHFR-G8)	CVCL_2441 (E111)
CVCL_6D48 (EGFR-T17)	CVCL_5741 (EJ-6-2-Bam-6a)	CVCL_U422 (Flp-In-3T3)
CVCL_WS34 (GhrasT-NIH/Swiss)	CVCL_8956 (GP+E-86)	CVCL_8957 (GP+envAM-12)
CVCL_D576 (HER14)	CVCL_4347 (II b)	CVCL_6662 (K:Molv NIH/3T3)
CVCL_KS91 (MEF/3T3 Tet-Off)	CVCL_U510 (MG87)	CVCL_Y512 (MOP-6)
CVCL_2617 (MOP-8)	CVCL_D900 (NEF)	CVCL_5I45 (NIH 2/4)
CVCL_QY95 (NIH 3T3 Atg5 KO)	CVCL_HC50 (NIH 3T3 Duo Parental)	CVCL_L994 (NIH 3T3 p-7 cl-3)
CVCL_D675 (NIH 3T3-IL-7)	CVCL_5845 (NIH 3T3-ras)	CVCL_9116 (NIH 3T3-TK-)
CVCL_0R23 (NIH 3T3/DC-SIGN)	CVCL_LG16 (NIH 3T3/HER2-3(400))	CVCL_0R24 (NIH 3T3/L-SIGN)
CVCL_7219 (NIH-3T3 4-2)	CVCL_7220 (NIH-3T3 clone 5611)	CVCL_L990 (NIH-3T3-A14)
CVCL_L991 (NIH-3T3-G185)	CVCL_7218 (NIH-3T3D4)	CVCL_V360 (NIH/3T3 Tet-On 3G)
CVCL_L992 (NIH3T3-3)	CVCL_VA35 (NIH3T3-47)	CVCL_M025 (NIH3T3/13C7)
CVCL_L995 (NIH3T3/14-1)	CVCL_4Y01 (NIH3T3/ATCC/CMV-Luc)	CVCL_JY97 (NIH3T3/GFP)
CVCL_KS45 (NIH3T3/NFkB-luc)	CVCL_D206 (NWT-21)	CVCL_2151 (OMEGA-E)
CVCL_2165 (Psi-2)	CVCL_W642 (Psi-AM22b)	CVCL_4278 (Psi-CRE)
CVCL_4279 (Psi-CRIP)	CVCL_LK41 (RL7)	CVCL_9Y75 (Sb51)
CVCL_2721 (Shh Light II)	CVCL_0552 (Swiss-LAPA)	CVCL_3433 (WOP)

Sex of cell

Male

Age at sampling

Embryo

Category

Spontaneously immortalized cell line

STR profile

Source(s): PubMed=23430347; PubMed=31220119

Markers:

Mouse STR 1-1	10
Mouse STR 1-2	13,17
Mouse STR 2-1	9
Mouse STR 3-2	14,15
Mouse STR 4-2	19.3,20.3
Mouse STR 5-5	14,15
Mouse STR 6-4	15.3
Mouse STR 6-7	12
Mouse STR 7-1	29
Mouse STR 8-1	15
Mouse STR 9-2	15,16
Mouse STR 11-2	15,17
Mouse STR 12-1	20
Mouse STR 13-1	16.2
Mouse STR 15-3	20.3
Mouse STR 17-2	13,14
Mouse STR 18-3	17,19
Mouse STR 19-2	11,12
Mouse STR X-1	25

Web pages

https://en.wikipedia.org/wiki/3T3_cells
http://nih3t3.com/
http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
https://www.atcc.org/support/faqs/89140/ATCC%20CCL92%20vs%20ATCC%20CRL1658-453.aspx
http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
http://www.proteinatlas.org/learn/cellines
http://www.cellresource.cn/fdetail.aspx?id=18
http://www.cellresource.cn/fdetail.aspx?id=406
http://www.cellresource.cn/fdetail.aspx?id=494
http://www.cellresource.cn/fdetail.aspx?id=798

Publications

PubMed=4311790
Jainchill J.L., Aaronson S.A., Todaro G.J.
Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
J. Virol. 4:549-553(1969)

PubMed=2463382
Raptis L., Bolen J.B.
Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
J. Virol. 63:753-758(1989)

PubMed=9753870
Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S., Moni M.S., Rajkumar T., Deshmukh S.
Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Indian J. Biochem. Biophys. 35:108-114(1998)

PubMed=23321776; DOI=10.1369/0022155413476868
Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A., Liehr T.
First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847-847(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

Cross-references

Cell line collections

AddexBio; P0011001/83
ATCC; CRL-1658
ATCC; CRL-6442 - Discontinued
BCRC; 60008
BCRJ; 0191
CCLV; CCLV-RIE 0058
CCTCC; GDC0030
CLS; 400101/p677_NIH-3T3
DSMZ; ACC-59
ECACC; 93061524
IBRC; C10100
ICLC; AL07001
IZSLER; BS CL 165
KCB; KCB 200645YJ
KCLB; 21658
NCBI_Iran; C156
NIH-ARP; 9946-386
RCB; RCB2767
TKG; TKG 0299

Cell line databases/resources

CLDB; cl3702
CLDB; cl3704
CLDB; cl3705
CLDB; cl3706
CLDB; cl3707
CLDB; cl3708
CLDB; cl3709
CLDB; cl3710
CLDB; cl3711
CLDB; cl4451
CLDB; cl7203
CCRID; 3111C0001CCC000018
CCRID; 3111C0002000000034
CCRID; 3131C0001000300006
CCRID; 3142C0001000000030
Lonza; 115
TOKU-E; 2753
TOKU-E; 3547

Ontologies