Cellosaurus Calu-3 (CVCL_0609)

Cell line name Calu-3
Synonyms CaLu-3; CALU-3; Calu 3; Calu3; CALU3
Accession CVCL_0609
Resource Identification Initiative To cite this cell line use: Calu-3 (RRID:CVCL_0609)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
From: Memorial Sloan-Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan-Kettering Cancer Center Office of Technology Development; SK1980-533.
Doubling time: 35 hours (in RPMI 1640+10% FBS), 45 hours (in ACL-3), 37 hours (in ACL-3+BSA) (PubMed=3940644).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Cell surface proteome.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: CALV3; In Cosmic 1434969.
Disease Lung adenocarcinoma (NCIt: C3512)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_EQ19 (Calu3-GemR)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; Cosmic-CLP; KCLB; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D13S31712
D16S53912,14
D18S5114,17
D21S1128,30
D3S135815,18
D5S81811
D7S82010,11
D8S117911,15
FGA25
Penta D9,16
Penta E5,21
TH016,9.3
TPOX8
vWA16,17
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-604.html
https://www.mskcc.org/research-advantage/support/technology/tangible-material/calu-3-human-lung-cell-line
http://tcpaportal.org/mclp/
Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3940644
Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.
Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Cancer Res. 46:798-806(1986)

PubMed=7515578
Shen B.Q., Finkbeiner W.E., Wine J.J., Mrsny R.J., Widdicombe J.H.
Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl-secretion.
Am. J. Physiol. 266:L493-L501(1994)

PubMed=10523844; DOI=10.1038/sj.onc.1202957
Agochiya M., Brunton V.G., Owens D.W., Parkinson E.K., Paraskeva C., Keith W.N., Frame M.C.
Increased dosage and amplification of the focal adhesion kinase gene in human cancer cells.
Oncogene 18:5646-5653(1999)

PubMed=11064206; DOI=10.1016/S0378-5173(00)00452-X
Foster K.A., Avery M.L., Yazdanian M., Audus K.L.
Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.
Int. J. Pharm. 208:1-11(2000)

PubMed=15463957; DOI=10.1016/j.jcf.2004.05.040
Gruenert D.C., Willems M., Cassiman J.J., Frizzell R.A.
Established cell lines used in cystic fibrosis research.
J. Cyst. Fibros. 3:191-196(2004)

PubMed=17483357; DOI=10.1158/0008-5472.CAN-06-4495
Tooker P., Yen W.-C., Ng S.-C., Negro-Vilar A., Hermann T.W.
Bexarotene (LGD1069, Targretin), a selective retinoid X receptor agonist, prevents and reverses gemcitabine resistance in NSCLC cells by modulating gene amplification.
Cancer Res. 67:4425-4433(2007)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24207061; DOI=10.1186/1559-0275-10-16
Cerciello F., Choi M., Nicastri A., Bausch-Fluck D., Ziegler A., Vitek O., Felley-Bosco E., Stahel R.A., Aebersold R., Wollscheid B.
Identification of a seven glycopeptide signature for malignant pleural mesothelioma in human serum by selected reaction monitoring.
Clin. Proteomics 10:16-16(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25555374; DOI=10.1016/j.ejps.2014.12.017
Kreft M.E., Jerman U.D., Lasic E., Hevir-Kene N., Rizner T.L., Peternel L., Kristan K.
The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.
Eur. J. Pharm. Sci. 69:1-9(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0016001/30
ATCC; HTB-55
BCRJ; 0264
IZSLER; BS TCL 103
KCLB; 30055
Cell line databases/resources CLDB; cl634
CLDB; cl635
CLDB; cl5176
CCLE; CALU3_LUNG
CCRID; 3111C0001CCC000032
CCRID; 3111C0001CCC000329
CCRID; 3131C0001000700067
CCRID; 3131C0001000700157
Cosmic-CLP; 687777
GDSC; 687777
IGRhCellID; Calu3
LINCS_HMS; 50010
LINCS_LDP; LCL-1631
Lonza; 342
TOKU-E; 768
Ontologies BTO; BTO:0002750
CLO; CLO_0002192
EFO; EFO_0002819
Biological sample resources BioSample; SAMN03471552
Chemistry resources ChEMBL-Cells; CHEMBL3307390
ChEMBL-Targets; CHEMBL614534
Gene expression databases GEO; GSM159355
GEO; GSM159356
GEO; GSM159357
GEO; GSM159358
GEO; GSM253347
GEO; GSM353233
GEO; GSM434276
GEO; GSM513948
GEO; GSM514318
GEO; GSM784249
GEO; GSM794262
GEO; GSM827549
GEO; GSM886915
GEO; GSM887981
GEO; GSM1374433
GEO; GSM1374434
GEO; GSM1669658
Polymorphism and mutation databases Cosmic; 687777
Cosmic; 910562
Cosmic; 933578
Cosmic; 945227
Cosmic; 961842
Cosmic; 1006532
Cosmic; 1028940
Cosmic; 1146873
Cosmic; 1219068
Cosmic; 1239869
Cosmic; 1434969
Cosmic; 1802295
Cosmic; 2125219
Proteomic databases PRIDE; PXD000589
PRIDE; PRD000594