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Cellosaurus MDA-MB-435S (CVCL_0622)

[Text version]

Cell line name MDA-MB-435S
Synonyms MDA-MB-435s; MDA-MB-435 S; MDA-MB-435-S; MDAMB435S; BrCL15
Accession CVCL_0622
Resource Identification Initiative To cite this cell line use: MDA-MB-435S (RRID:CVCL_0622)
Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00565.
Microsatellite instability: Stable (MSS) (PubMed=12661003).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: miRNA expression profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Heterozygous for BRAF p.Val600Glu (c.1799T>A) (PubMed=19593635).
Heterozygous for CDKN2A c.150+2T>C (IVS1+2T>C); splice donor mutation and c.455insCdel26 (PubMed=19593635).
Hemizygous for EP300 p.Leu827Pro (c.3679T>C) and p.Glu1013Gly (4237A>G) (PubMed=10700188).
Heterozygous for TP53 p.Gly266Glu (c.797G>A) (PubMed=16541312).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.01
Native American0
East Asian, North2.13
East Asian, South0
South Asian0
European, North65.23
European, South32.63
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Subcutaneous; right buttock.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0417 (MDA-MB-435)
Children:
CVCL_5T70 (MDA-MB-435s-mKate2)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; PubMed=28940260

Markers:
AmelogeninX
CSF1PO11 (ATCC; CLS)
11,12 (PubMed=28940260)
D2S133819,24
D3S135814
D5S81812
D7S8208,10 (ATCC; CLS)
8 (PubMed=28940260)
D8S117913
D13S31712
D16S53913
D18S5113,17
D19S43314,15
D21S1130
FGA21
Penta D9,11
Penta E10,12
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/support/faqs/91699/ATCC%20HTB-129%20Derivative-1051.aspx
https://www.atcc.org/support/faqs/79d25/Morphology%20of%20ATCC%20HTB-129-495.aspx
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

DOI=10.1016/B978-0-12-333530-2.50009-5
Leibovitz A.
Cell lines from human breast.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York (1994)

PubMed=10700188; DOI=10.1038/73536
Gayther S.A., Batley S.J., Linger L., Bannister A., Thorpe K., Chin S.-F., Daigo Y., Russell P., Wilson A., Sowter H.M., Delhanty J.D.A., Ponder B.A.J., Kouzarides T., Caldas C.
Mutations truncating the EP300 acetylase in human cancers.
Nat. Genet. 24:300-303(2000)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.-F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853
Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., van den Ouweland A., Merajver S.D., Ethier S.P., Schutte M.
BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.
Cancer Res. 66:41-45(2006)

PubMed=16541312; DOI=10.1007/s10549-006-9186-z
Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.
Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.
Breast Cancer Res. Treat. 99:97-101(2006)

PubMed=19593635; DOI=10.1007/s10549-009-0460-8
Hollestelle A., Nagel J.H.A., Smid M., Lam S., Elstrodt F., Wasielewski M., Ng S.S., French P.J., Peeters J.K., Rozendaal M.J., Riaz M., Koopman D.G., ten Hagen T.L.M., de Leeuw B.H.C.G.M., Zwarthoff E.C., Teunisse A., van der Spek P.J., Klijn J.G.M., Dinjens W.N.M., Ethier S.P., Clevers H., Jochemsen A.G., den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.
Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.
Breast Cancer Res. Treat. 121:53-64(2010)

PubMed=20070913; DOI=10.1186/1471-2407-10-15
Tsuji K., Kawauchi S., Saito S., Furuya T., Ikemoto K., Nakao M., Yamamoto S., Oka M., Hirano T., Sasaki K.
Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.
BMC Cancer 10:15-15(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23601657; DOI=10.1186/bcr3415
Riaz M., van Jaarsveld M.T.M., Hollestelle A., Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J., Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A.C., Foekens J.A., Martens J.W.M.
miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.
Breast Cancer Res. 15:R33-R33(2013)

PubMed=28940260; DOI=10.1002/ijc.31067
Korch C., Hall E.M., Dirks W.G., Ewing M., Faries M., Varella-Garcia M., Robinson S., Storts D.R., Turner J.A., Wang Y., Burnett E.C., Healy L., Kniss D., Neve R.M., Nims R.W., Reid Y.A., Robinson W.A., Capes-Davis A.
Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
Int. J. Cancer 142:561-572(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections ATCC; HTB-129
BCRJ; 0165
CCTCC; GDC0053
CLS; 300277/NA
ICLC; HTL03006
Cell line databases/resources CLDB; cl7142
CCLE; MDAMB435S_SKIN
CCRID; 3111C0001CCC000015
CCRID; 3131C0001000700036
CCRID; 3142C0001000000052
Cell_Model_Passport; SIDM01222
DepMap; ACH-000884
LINCS_HMS; 50030
LINCS_LDP; LCL-1307
Ontologies BTO; BTO:0003941
CLO; CLO_0007638
Biological sample resources BioSample; SAMN10987989
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSM149983
GEO; GSM149991
GEO; GSM149999
GEO; GSM421877
GEO; GSM887298
GEO; GSM888373
GEO; GSM1374657
Other Wikidata; Q54904637
Polymorphism and mutation databases Cosmic; 809237
Cosmic; 871155
Cosmic; 894089
Cosmic; 904381
Cosmic; 1044202
Cosmic; 1046951
Cosmic; 1287922
Cosmic; 1289399
Cosmic; 1523969
Cosmic; 1609477
Cosmic; 2301531
LiGeA; CCLE_408
Entry history
Entry creation04-Apr-2012
Last entry updated05-Jul-2019
Version number27