ID   SK-ES-1
AC   CVCL_0627
SY   Sk-Es-1; Sk-ES-1; SK ES 01; SK-ES1; SKES-1; SKES1; SK-ES
DR   CLO; CLO_0009036
DR   EFO; EFO_0002858
DR   ArrayExpress; E-MTAB-2770
DR   ArrayExpress; E-MTAB-3610
DR   ATCC; HTB-86
DR   BCRJ; 0266
DR   BioSample; SAMN03472758
DR   BioSample; SAMN03473516
DR   BioSample; SAMN10987953
DR   CCLE; SKES1_BONE
DR   Cell_Model_Passport; SIDM01111
DR   ChEMBL-Cells; CHEMBL3307826
DR   ChEMBL-Targets; CHEMBL613970
DR   CLS; 300435/p738_SK-ES-1
DR   Cosmic; 684072
DR   Cosmic; 759888
DR   Cosmic; 1078385
DR   Cosmic; 1082505
DR   Cosmic; 1085904
DR   Cosmic; 1388894
DR   Cosmic; 1718430
DR   Cosmic; 1995629
DR   Cosmic; 2060800
DR   Cosmic; 2228231
DR   Cosmic; 2250467
DR   Cosmic-CLP; 684072
DR   DepMap; ACH-000087
DR   DSMZ; ACC-518
DR   GDSC; 684072
DR   GEO; GSM393027
DR   GEO; GSM510008
DR   GEO; GSM510017
DR   GEO; GSM510018
DR   GEO; GSM827589
DR   GEO; GSM887577
DR   GEO; GSM888660
DR   GEO; GSM1670428
DR   GEO; GSM1676343
DR   GEO; GSM1701674
DR   IARC_TP53; 710
DR   IGRhCellID; SKES%20%20GEO
DR   LiGeA; CCLE_862
DR   LINCS_LDP; LCL-1434
DR   PharmacoDB; SKES1_1387_2019
DR   Wikidata; Q54953654
RX   PubMed=327080;
RX   PubMed=833871;
RX   PubMed=3518877;
RX   PubMed=6220172;
RX   PubMed=8040301;
RX   PubMed=8221663;
RX   PubMed=8617485;
RX   PubMed=9738976;
RX   PubMed=11423975;
RX   PubMed=11891184;
RX   PubMed=12054564;
RX   PubMed=18160777;
RX   PubMed=19787792;
RX   PubMed=20215515;
RX   PubMed=22142829;
RX   PubMed=22460905;
RX   PubMed=25010205;
RX   PubMed=25223734;
RX   PubMed=26351324;
RX   PubMed=27397505;
RX   PubMed=28196595;
RX   PubMed=30894373;
RX   PubMed=31068700;
RX   PubMed=31978347;
WW   https://www.mskcc.org/research-advantage/support/technology/tangible-material/sk-es-1-human-ewing-sarcoma-cell-line
WW   http://tcpaportal.org/mclp/
CC   Part of: Cancer Cell Line Encyclopedia (CCLE) project.
CC   Part of: COSMIC cell lines project.
CC   Part of: MD Anderson Cell Lines Project.
CC   From: Memorial Sloan Kettering Cancer Center; New York; USA.
CC   Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-542.
CC   Doubling time: ~35 hours (DSMZ).
CC   Microsatellite instability: Stable (MSS) (Sanger).
CC   Sequence variation: EWSR1-FLI1 (EWS-FLI1) gene fusion (PubMed=8040301; PubMed=9738976; PubMed=11891184).
CC   Sequence variation: Homozygous for CDKN2A deletion (PubMed=18160777; PubMed=19787792).
CC   Sequence variation: STAG2 p.Gln735Ter (c.2203C>T) (PubMed=25010205; PubMed=25223734).
CC   Sequence variation: TP53 p.Cys176Phe (c.527G>T) (ClinVar=VCV000376569) (PubMed=8221663; PubMed=19787792; PubMed=25010205; PubMed=25223734).
CC   Omics: Array-based CGH.
CC   Omics: Deep exome analysis.
CC   Omics: Deep quantitative proteome analysis.
CC   Omics: Deep RNAseq analysis.
CC   Omics: DNA methylation analysis.
CC   Omics: Protein expression by reverse-phase protein arrays.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis.
CC   Genome ancestry: African=0.13%; Native American=0%; East Asian, North=0.63%; East Asian, South=0%; South Asian=1.64%; European, North=63.44%; European, South=34.15% (PubMed=30894373).
CC   Miscellaneous: The signal for the amelogenin "Y" allele is quite weak (MSKCC).
ST   Source(s): ATCC; CLS; Cosmic-CLP; DSMZ; MSKCC; PubMed=19787792; PubMed=25877200
ST   Amelogenin: X,Y
ST   CSF1PO: 11
ST   D13S317: 8,9
ST   D16S539: 11 (ATCC; CLS; Cosmic-CLP; MSKCC; PubMed=19787792; PubMed=25877200)
ST   D16S539: 12 (DSMZ)
ST   D18S51: 13,15
ST   D19S433: 13,14
ST   D21S11: 30
ST   D2S1338: 17
ST   D3S1358: 16,18
ST   D5S818: 12
ST   D7S820: 10,11
ST   D8S1179: 11,13
ST   FGA: 20,21
ST   Penta D: 11,13
ST   Penta E: 11
ST   SE33: 18,20
ST   TH01: 6,9.3
ST   TPOX: 8
ST   vWA: 14,17
DI   NCIt; C4817; Ewing sarcoma
DI   ORDO; Orphanet_319; Ewing sarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens
SX   Male
AG   18Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 02-07-20; Version: 28
//
RX   PubMed=327080; DOI=10.1093/jnci/59.1.221;
RA   Fogh J., Fogh J.M., Orfeo T.;
RT   "One hundred and twenty-seven cultured human tumor cell lines
RT   producing tumors in nude mice.";
RL   J. Natl. Cancer Inst. 59:221-226(1977).
//
RX   PubMed=833871; DOI=10.1093/jnci/58.2.209;
RA   Fogh J., Wright W.C., Loveless J.D.;
RT   "Absence of HeLa cell contamination in 169 cell lines derived from
RT   human tumors.";
RL   J. Natl. Cancer Inst. 58:209-214(1977).
//
RX   PubMed=3518877; DOI=10.3109/07357908609038260;
RA   Fogh J.;
RT   "Human tumor lines for cancer research.";
RL   Cancer Invest. 4:157-184(1986).
//
RX   PubMed=6220172;
RA   Dracopoli N.C., Fogh J.;
RT   "Polymorphic enzyme analysis of cultured human tumor cell lines.";
RL   J. Natl. Cancer Inst. 70:469-476(1983).
//
RX   PubMed=8040301; DOI=10.1172/JCI117360;
RA   Giovannini M., Biegel J.A., Serra M., Wang J.-Y., Wei Y.H., Nycum L.,
RA   Emanuel B.S., Evans G.A.;
RT   "EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and
RT   primitive neuroectodermal tumors with variant translocations.";
RL   J. Clin. Invest. 94:489-496(1994).
//
RX   PubMed=8221663;
RA   Komuro H., Hayashi Y., Kawamura M., Hayashi K., Kaneko Y.,
RA   Kamoshita S., Hanada R., Yamamoto K., Hongo T., Yamada M., Tsuchida Y.;
RT   "Mutations of the p53 gene are involved in Ewing's sarcomas but not in
RT   neuroblastomas.";
RL   Cancer Res. 53:5284-5288(1993).
//
RX   PubMed=8617485; DOI=10.1016/S0046-8177(96)90115-X;
RA   Scotlandi K., Serra M., Manara M.C., Benini S., Sarti M., Maurici D.,
RA   Lollini P.-L., Picci P., Bertoni F., Baldini N.;
RT   "Immunostaining of the p30/32MIC2 antigen and molecular detection of
RT   EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral
RT   neuroectodermal tumor.";
RL   Hum. Pathol. 27:408-416(1996).
//
RX   PubMed=9738976; DOI=10.1111/j.1349-7006.1998.tb03274.x;
RA   Urano F., Umezawa A., Yabe H., Hong W., Yoshida K., Fujinaga K.,
RA   Hata J.-I.;
RT   "Molecular analysis of Ewing's sarcoma: another fusion gene, EWS-E1AF,
RT   available for diagnosis.";
RL   Jpn. J. Cancer Res. 89:703-711(1998).
//
RX   PubMed=11423975; DOI=10.1038/sj.onc.1204437;
RA   Dauphinot L., De Oliveira C., Melot T., Sevenet N., Thomas V.,
RA   Weissman B.E., Delattre O.;
RT   "Analysis of the expression of cell cycle regulators in Ewing cell
RT   lines: EWS-FLI-1 modulates p57KIP2and c-Myc expression.";
RL   Oncogene 20:3258-3265(2001).
//
RX   PubMed=11891184; DOI=10.1016/S0002-9440(10)64908-5;
RA   Manara M.C., Perbal B., Benini S., Strammiello R., Cerisano V.,
RA   Perdichizzi S., Serra M., Astolfi A., Bertoni F., Alami J., Yeger H.,
RA   Picci P., Scotlandi K.;
RT   "The expression of ccn3(nov) gene in musculoskeletal tumors.";
RL   Am. J. Pathol. 160:849-859(2002).
//
RX   PubMed=12054564; DOI=10.1016/S0006-291X(02)00129-8;
RA   Yabe H., Fukuma M., Urano F., Yoshida K., Kato S., Toyama Y., Hata J.-I.,
RA   Umezawa A.;
RT   "Lack of matrix metalloproteinase (MMP)-1 and -3 expression in Ewing
RT   sarcoma may be due to loss of accessibility of the MMP regulatory
RT   element to the specific fusion protein in vivo.";
RL   Biochem. Biophys. Res. Commun. 293:61-71(2002).
//
RX   PubMed=18160777; DOI=10.1159/000109614;
RA   Savola S., Nardi F., Scotlandi K., Picci P., Knuutila S.;
RT   "Microdeletions in 9p21.3 induce false negative results in CDKN2A FISH
RT   analysis of Ewing sarcoma.";
RL   Cytogenet. Genome Res. 119:21-26(2007).
//
RX   PubMed=19787792; DOI=10.1002/gcc.20717;
RA   Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S.,
RA   Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H.,
RA   Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W.,
RA   Buerger H., Aigner T., Gabbert H.E., Poremba C.;
RT   "Molecular characterization of commonly used cell lines for bone tumor
RT   research: a trans-European EuroBoNet effort.";
RL   Genes Chromosomes Cancer 49:40-51(2010).
//
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458;
RA   Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P.,
RA   Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J.,
RA   Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C.,
RA   Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J.,
RA   Haber D.A.;
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
//
RX   PubMed=22142829; DOI=10.1158/1078-0432.CCR-11-2056;
RA   Shukla N., Ameur N., Yilmaz I., Nafa K., Lau C.-Y., Marchetti A.,
RA   Borsu L., Barr F.G., Ladanyi M.;
RT   "Oncogene mutation profiling of pediatric solid tumors reveals
RT   significant subsets of embryonal rhabdomyosarcoma and neuroblastoma
RT   with mutated genes in growth signaling pathways.";
RL   Clin. Cancer Res. 18:748-757(2012).
//
RX   PubMed=22460905; DOI=10.1038/nature11003;
RA   Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A.,
RA   Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A.,
RA   Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J.,
RA   Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E.,
RA   Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J.,
RA   Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C.,
RA   Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C.,
RA   Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P.,
RA   Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L.,
RA   Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R.,
RA   Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.;
RT   "The Cancer Cell Line Encyclopedia enables predictive modelling of
RT   anticancer drug sensitivity.";
RL   Nature 483:603-607(2012).
//
RX   PubMed=25010205; DOI=10.1371/journal.pgen.1004475;
RA   Brohl A.S., Solomon D.A., Chang W., Wang J., Song Y., Sindiri S.,
RA   Patidar R., Hurd L., Chen L., Shern J.F., Liao H., Wen X., Gerard J.,
RA   Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P.,
RA   Triche T.J., Horvai A.E., Miettinen M., Wei J.S., Catchpoole D.,
RA   Llombart-Bosch A., Waldman T., Khan J.;
RT   "The genomic landscape of the Ewing sarcoma family of tumors reveals
RT   recurrent STAG2 mutation.";
RL   PLoS Genet. 10:E1004475-E1004475(2014).
//
RX   PubMed=25223734; DOI=10.1158/2159-8290.CD-14-0622;
RA   Tirode F., Surdez D., Ma X., Parker M., Le Deley M.C., Bahrami A.,
RA   Zhang Z., Lapouble E., Grossetete-Lalami S., Rusch M., Reynaud S.,
RA   Rio-Frio T., Hedlund E., Wu G., Chen X., Pierron G., Oberlin O.,
RA   Zaidi S., Lemmon G., Gupta P., Vadodaria B., Easton J., Gut M.,
RA   Ding L., Mardis E.R., Wilson R.K., Shurtleff S., Laurence V.,
RA   Michon J., Marec-Berard P., Gut I.G., Downing J., Dyer M., Zhang J.,
RA   Delattre O.;
RT   "Genomic landscape of Ewing sarcoma defines an aggressive subtype with
RT   co-association of STAG2 and TP53 mutations.";
RL   Cancer Discov. 4:1342-1353(2014).
//
RX   PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074;
RA   Teicher B.A., Polley E.C., Kunkel M., Evans D., Silvers T.E.,
RA   Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J.,
RA   Harris E., Monks A., Morris J.;
RT   "Sarcoma cell line screen of oncology drugs and investigational agents
RT   identifies patterns associated with gene and microRNA expression.";
RL   Mol. Cancer Ther. 14:2452-2462(2015).
//
RX   PubMed=27397505; DOI=10.1016/j.cell.2016.06.017;
RA   Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P.,
RA   Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H.,
RA   Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H.,
RA   Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X.,
RA   Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M.,
RA   Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M.,
RA   Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A.,
RA   Saez-Rodriguez J., McDermott U., Garnett M.J.;
RT   "A landscape of pharmacogenomic interactions in cancer.";
RL   Cell 166:740-754(2016).
//
RX   PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005;
RA   Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P.,
RA   Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W.,
RA   Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D.,
RA   Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B.,
RA   Liang H.;
RT   "Characterization of human cancer cell lines by reverse-phase protein
RT   arrays.";
RL   Cancer Cell 31:225-239(2017).
//
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747;
RA   Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.;
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3;
RA   Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C.,
RA   McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H.,
RA   Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F.,
RA   Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R.,
RA   Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C.,
RA   Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A.,
RA   Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D.,
RA   Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L.,
RA   Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A.,
RA   Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D.,
RA   Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M.,
RA   Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R.,
RA   Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A.,
RA   Sellers W.R.;
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//
RX   PubMed=31978347; DOI=10.1016/j.cell.2019.12.023;
RA   Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III,
RA   Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K.,
RA   Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K.,
RA   Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A.,
RA   Sellers W.R., Gygi S.P.;
RT   "Quantitative proteomics of the Cancer Cell Line Encyclopedia.";
RL   Cell 180:387-402.e16(2020).
//