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Cellosaurus U-118MG (CVCL_0633)

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Cell line name U-118MG
Synonyms U-118 MG; U-118-MG; U118-MG; U118MG; U118; 118 MG; 118MG
Accession CVCL_0633
Resource Identification Initiative To cite this cell line use: U-118MG (RRID:CVCL_0633)
Comments Problematic cell line: Contaminated. Shown to be a U-138MG derivative (PubMed=20143388).
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00350.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Has no IDH1 mutation (PubMed=19435942).
Homozygous for PTEN c.1026+1G>T (IVS8+1G>T); splice donor mutation (PubMed=10560660; Cosmic-CLP).
Homozygous for TP53 p.Arg213Gln (c.638G>A) (PubMed=10416987; Cosmic-CLP).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.71
Native American0
East Asian, North1.75
East Asian, South0
South Asian0
European, North96.54
European, South0
Disease Astrocytoma (NCIt: C60781)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0020 (U-138MG)
Children:
CVCL_D317 (1181N1)CVCL_T447 (EH-118MG)CVCL_S097 (KC)
Sex of cell Male
Age at sampling 47Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; PubMed=22570425; PubMed=27582061

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133823,25
D3S135815
D5S81811
D7S8209
D8S117914,15
D13S3179 (ATCC; Cosmic-CLP)
9,11 (CLS; PubMed=22570425; PubMed=27582061)
D16S53912,13
D18S5113
D19S43313
D21S1127,32.2
FGA23 (CLS; PubMed=22570425)
18,23 (PubMed=27582061)
Penta D13 (CLS)
10,13 (PubMed=22570425; PubMed=27582061)
Penta E7 (CLS; PubMed=22570425)
7,13 (PubMed=27582061)
TH016
TPOX8
vWA18

Run an STR similarity search on this cell line
Web pages https://strap.nci.nih.gov/celline_detail.php?sample_id=75
https://www.atcc.org/support/faqs/931ae/U118%20MG%20ATCC%20HTB15%20U138%20MG%20ATCC%20HTB16-1056.aspx
http://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/u118/u118cells.html
http://www.cellresource.cn/fdetail.aspx?id=1603
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

PubMed=4337530; DOI=10.1111/j.1699-0463.1972.tb02176.x
Macintyre E.H., Ponten J., Vatter A.E.
The ultrastructure of human and murine astrocytes and of human fibroblasts in culture.
Acta Pathol. Microbiol. Scand. A. 80:267-283(1972)

PubMed=4134536; DOI=10.1002/ijc.2910120215
Westermark B.
The deficient density-dependent growth control of human malignant glioma cells and virus-transformed glia-like cells in culture.
Int. J. Cancer 12:438-451(1973)

PubMed=4369403; DOI=10.1002/1097-0142(197408)34:2<274::AID-CNCR2820340209>3.0.CO;2-4
Wahlstrom T., Linder E., Saksela E., Westermark B.
Tumor-specific membrane antigens in established cell lines from gliomas.
Cancer 34:274-279(1974)

DOI=10.1007/978-1-4757-1647-4_7
Ponten J.
Neoplastic human glia cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.175-206; Springer; New York (1975)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=450131; DOI=10.1038/279797a0
Day R.S. III, Ziolkowski C.H.J.
Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5.
Nature 279:797-799(1979)

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Bigner D.D., Bigner S.H., Ponten J., Westermark B., Mahaley M.S. Jr., Ruoslahti E., Herschman H., Eng L.F., Wikstrand C.J.
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003
Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2454731
Nister M., Libermann T.A., Betsholtz C., Pettersson M., Claesson-Welsh L., Heldin C.-H., Schlessinger J., Westermark B.
Expression of messenger RNAs for platelet-derived growth factor and transforming growth factor-alpha and their receptors in human malignant glioma cell lines.
Cancer Res. 48:3910-3918(1988)

PubMed=2253244
Bigner S.H., Humphrey P.A., Wong A.J., Vogelstein B., Mark J., Friedman H.S., Bigner D.D.
Characterization of the epidermal growth factor receptor in human glioma cell lines and xenografts.
Cancer Res. 50:8017-8022(1990)

DOI=10.1016/B978-0-12-333530-2.50005-8
Nister M., Westermark B.
Human glioma cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York (1994)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Sharif T.R., Luo W., Sharif M.
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x
Ishii N., Maier D., Merlo A., Tada M., Sawamura Y., Diserens A.-C., Van Meir E.G.
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=10560660; DOI=10.1097/00005072-199911000-00007
Schmidt E.E., Ichimura K., Goike H.M., Moshref A., Liu L., Collins V.P.
Mutational profile of the PTEN gene in primary human astrocytic tumors and cultivated xenografts.
J. Neuropathol. Exp. Neurol. 58:1170-1183(1999)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=16697959; DOI=10.1016/j.ccr.2006.03.030
Lee J., Kotliarova S., Kotliarov Y., Li A., Su Q., Donin N.M., Pastorino S., Purow B.W., Christopher N., Zhang W., Park J.K., Fine H.A.
Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.
Cancer Cell 9:391-403(2006)

PubMed=19365568; DOI=10.1371/journal.pone.0005209
Bax D.A., Little S.E., Gaspar N., Perryman L., Marshall L., Viana-Pereira M., Jones T.A., Williams R.D., Grigoriadis A., Vassal G., Workman P., Sheer D., Reis R.M., Pearson A.D.J., Hargrave D., Jones C.
Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development.
PLoS ONE 4:E5209-E5209(2009)

PubMed=19435942; DOI=10.1215/15228517-2009-025
Ichimura K., Pearson D.M., Kocialkowski S., Backlund L.M., Chan R., Jones D.T.W., Collins V.P.
IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas.
Neuro-oncol. 11:341-347(2009)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22570425; DOI=10.1093/neuonc/nos072
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27582061; DOI=10.1126/scitranslmed.aaf6853
Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.
Origin of the U87MG glioma cell line: good news and bad news.
Sci. Transl. Med. 8:354re3-354re3(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections ATCC; HTB-15
ATCC; CRL-7712 - Discontinued
CLS; 300362/p4581_U-118_MG
KCB; KCB 201302YJ
Cell line databases/resources CCLE; U118MG_CENTRAL_NERVOUS_SYSTEM
CCRID; 3131C0001001200022
Cell_Model_Passport; SIDM01193
Cosmic-CLP; 687588
DepMap; ACH-000040
GDSC; 687588
LINCS_LDP; LCL-1358
Ontologies BTO; BTO:0004605
CLO; CLO_0009451
Biological sample resources BioSample; SAMN03151974
BioSample; SAMN03472723
BioSample; SAMN10988042
Chemistry resources ChEMBL-Cells; CHEMBL3308793
ChEMBL-Targets; CHEMBL1075602
PharmacoDB; U118MG_1616_2019
Gene expression databases ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM101671
GEO; GSM101672
GEO; GSM888812
GEO; GSM1374968
GEO; GSM1374969
GEO; GSM1670554
Other Wikidata; Q54973503
Polymorphism and mutation databases Cosmic; 687588
Cosmic; 849852
Cosmic; 849878
Cosmic; 920817
Cosmic; 1175822
Cosmic; 1217672
Cosmic; 1571919
Cosmic; 1610738
Cosmic; 2301554
Cosmic; 2367553
Cosmic; 2550362
Cosmic; 2668299
Cosmic; 2701084
IARC_TP53; 4195
LiGeA; CCLE_075
Entry history
Entry creation04-Apr-2012
Last entry updated06-Sep-2019
Version number28