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Cellosaurus RN33B (CVCL_0E00)

Cell line name RN33B
Synonyms Rn33B
Accession CVCL_0E00
Resource Identification Initiative To cite this cell line use: RN33B (RRID:CVCL_0E00)
Comments Characteristics: Profiferates at the permissive temperature (33 Celsius). At the restrictive temperature (38.5 Celsius) ceases mitotic activity and differentiates with phase bright cell bodies and 'neuritic-like' processes.
Doubling time: 48 hours (PubMed=8364724).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA58].
Derived from site: In situ; Embryonic brain, medullary raphe nucleus; UBERON=UBERON_0002692.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley.
Hierarchy Children:
CVCL_C1L0 (Rn33B-A68B2M)CVCL_XF90 (RN33B.LV-AA2)
Sex of cell Sex unspecified
Age at sampling 12-13FD
Category Conditionally immortalized cell line
Publications

PubMed=8364724; DOI=10.1016/0006-8993(93)91111-5
Whittemore S.R., White L.A.
Target regulation of neuronal differentiation in a temperature-sensitive cell line derived from medullary raphe.
Brain Res. 615:27-40(1993)

PubMed=7905006; DOI=10.1016/0165-5728(94)90189-9
White L.A., Keane R.W., Whittemore S.R.
Differentiation of an immortalized CNS neuronal cell line decreases their susceptibility to cytotoxic T cell lysis in vitro.
J. Neuroimmunol. 49:135-143(1994)

PubMed=8699192; DOI=10.1007/BF02284789
Shihabuddin L.S., Brunschwig J.-P., Holets V.R., Bunge M.B., Whittemore S.R.
Induction of mature neuronal properties in immortalized neuronal precursor cells following grafting into the neonatal CNS.
J. Neurocytol. 25:101-111(1996)

PubMed=12061867; DOI=10.1006/exnr.2002.7888
Lundberg C., Englund U., Trono D., Bjorklund A., Wictorin K.
Differentiation of the RN33B cell line into forebrain projection neurons after transplantation into the neonatal rat brain.
Exp. Neurol. 175:370-387(2002)

PubMed=12112642; DOI=10.1002/jgm.268
Rosenqvist N., Hard af Segerstad C., Samuelsson C., Johansen J., Lundberg C.
Activation of silenced transgene expression in neural precursor cell lines by inhibitors of histone deacetylation.
J. Gene Med. 4:248-257(2002)

PubMed=15139013; DOI=10.1002/glia.20033
Wojciechowski A.B., Englund U., Lundberg C., Warfvinge K.
Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats.
Glia 47:58-67(2004)

PubMed=31758567; DOI=10.1111/1348-0421.12761
Minato E., Kobayashi A., Aoshima K., Fukushi H., Kimura T.
Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation dependent.
Microbiol. Immunol. 64:123-132(2020)

Cross-references
Cell line collections (Providers) ATCC; CRL-2825
Encyclopedic resources Wikidata; Q54950747
Entry history
Entry creation10-Apr-2015
Last entry update05-Oct-2023
Version number14