Cellosaurus C32 [Human melanoma] (CVCL_1097)

Cell line name C32 [Human melanoma]
Synonyms C-32; C32-mel; C32 mel; C32r
Accession CVCL_1097
Resource Identification Initiative To cite this cell line use: C32 [Human melanoma] (RRID:CVCL_1097)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MET genetic alteration cell panel (ATCC TCP-1036).
Part of: PTEN genetic alteration cell panel (ATCC TCP-1030).
Doubling time: 53 hours (PubMed=25984343).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: BRAF p.Val600Glu (PubMed=15048078).
Sequence variation: CDKN2A p.Ter157Ser (PubMed=9354451).
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_2324 (C32TG)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; Cosmic-CLP; ECACC; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO11,12
D13S31712
D16S53911,12
D18S5112,15
D21S1129
D3S135814,17
D5S81812
D7S8209,11
D8S117913,14
FGA24
Penta D9,13
Penta E12
TH019.3
TPOX9
vWA14,19
Publications

PubMed=3900210
Barnwell J.W., Ockenhouse C.F., Knowles D.M. II
Monoclonal antibody OKM5 inhibits the in vitro binding of Plasmodium falciparum-infected erythrocytes to monocytes, endothelial, and C32 melanoma cells.
J. Immunol. 135:3494-3497(1985)

PubMed=3182330; DOI=10.1016/0360-3016(88)90125-3
Schwartz J.L., Rotmensch J., Giovanazzi S., Cohen M.B., Weichselbaum R.R.
Faster repair of DNA double-strand breaks in radioresistant human tumor cells.
Int. J. Radiat. Oncol. Biol. Phys. 15:907-912(1988)

PubMed=9354451
Castellano M., Pollock P.M., Walters M.K., Sparrow L.E., Down L.M., Gabrielli B.G., Parsons P.G., Hayward N.K.
CDKN2A/p16 is inactivated in most melanoma cell lines.
Cancer Res. 57:4868-4875(1997)

PubMed=15048078; DOI=10.1038/sj.onc.1207563
Pavey S., Johansson P., Packer L., Taylor J., Stark M., Pollock P.M., Walker G.J., Boyle G.M., Harper U., Cozzi S.-J., Hansen K., Yudt L., Schmidt C., Hersey P., Ellem K.A.O., O'Rourke M.G.E., Parsons P.G., Meltzer P., Ringner M., Hayward N.K.
Microarray expression profiling in melanoma reveals a BRAF mutation signature.
Oncogene 23:4060-4067(2004)

PubMed=15467732; DOI=10.1038/sj.onc.1208152
Tanami H., Imoto I., Hirasawa A., Yuki Y., Sonoda I., Inoue J., Yasui K., Misawa-Furihata A., Kawakami Y., Inazawa J.
Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.
Oncogene 23:8796-8804(2004)

PubMed=17363583; DOI=10.1158/0008-5472.CAN-06-4152
Stark M., Hayward N.
Genome-wide loss of heterozygosity and copy number analysis in melanoma using high-density single-nucleotide polymorphism arrays.
Cancer Res. 67:2632-2642(2007)

PubMed=17516929; DOI=10.1111/j.1600-0749.2007.00375.x
Johansson P., Pavey S., Hayward N.
Confirmation of a BRAF mutation-associated gene expression signature in melanoma.
Pigment Cell Res. 20:216-221(2007)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

Cross-references
Cell line collections AddexBio; C0020002/443
ATCC; CRL-1585
BCRC; 60081
ECACC; 87090201
IZSLER; BS TCL 150
Cell line databases/resources CLDB; cl566
CLDB; cl5173
CCLE; C32_SKIN
CGH-DB; 9321-4
Cosmic-CLP; 906830
GDSC; 906830
LINCS_HMS; 50112
LINCS_LDP; LCL-1238
Ontologies CLO; CLO_0002113
EFO; EFO_0006364
Biological sample resources BioSample; SAMN03472821
Chemistry resources ChEMBL-Cells; CHEMBL3308713
ChEMBL-Targets; CHEMBL1075401
Gene expression databases GEO; GSM162931
GEO; GSM206449
GEO; GSM215721
GEO; GSM827471
GEO; GSM886898
GEO; GSM887963
GEO; GSM1138788
GEO; GSM1669636
Other Wikidata; Q27902869
Polymorphism and mutation databases Cosmic; 687432
Cosmic; 888988
Cosmic; 905234
Cosmic; 906830
Cosmic; 928725
Cosmic; 933006
Cosmic; 948845
Cosmic; 986005
Cosmic; 995374
Cosmic; 1022272
Cosmic; 1043555
Cosmic; 1155267
Cosmic; 1223131
Cosmic; 1312291
Cosmic; 1458960
Cosmic; 1479621
Cosmic; 1995352