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Cellosaurus LS174T (CVCL_1384)

Cell line name LS174T
Synonyms Ls174T; LS174t; Ls-174-T; LS-174-T; LS 174 T; LS-174T; Ls-174T; LS 174T; LS-174; LS174
Accession CVCL_1384
Secondary accession CVCL_D190
Resource Identification Initiative To cite this cell line use: LS174T (RRID:CVCL_1384)
Comments Part of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: MD Anderson Cell Lines Project.
Doubling time: ~30-40 hours (DSMZ).
Microsatellite instability: Instable (MSI) (PubMed=9000147; PubMed=10674020; PubMed=24042735; PubMed=25926053; PubMed=28683746).
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: O-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: LV1747; In Cosmic 1187310 and PubMed=19132987.
Misspelling: L5174T; Occasionally.
Sequence variations CTNNB1 p.Ser45Phe (c.134C>T) (ATCC; PubMed=24755471).
Heterozygous for KRAS p.Gly12Asp (c.35G>A) (PubMed=12068308; PubMed=20570890; PubMed=24042735; PubMed=24755471; PubMed=28683746).
PIK3CA p.His1047Arg (c.3140A>G) (PubMed=20570890; PubMed=24042735; PubMed=24755471; PubMed=28683746).
Has no TP53 mutation (PubMed=16418264; PubMed=24042735).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_D240 (LiM6)CVCL_0306 (LS174T-HM3)CVCL_6670 (LS174T-HM7)
CVCL_J074 (LS174T-W4)CVCL_2129 (MT-3 [Human contaminated breast cancer])CVCL_0H88 (S1)
Originate from same individual CVCL_0397 ! LS180
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; DSMZ; KCLB; PubMed=11416159; PubMed=25877200; PubMed=25926053; TKG

Markers:
AmelogeninX
CSF1PO10,13,14 (AddexBio; ATCC; PubMed=25926053)
10,11,14 (DSMZ)
10,14 (CLS; KCLB; PubMed=25877200; TKG)
D13S31710 (AddexBio; ATCC; PubMed=25877200; PubMed=25926053)
9,10,11 (DSMZ)
10,11 (CLS; KCLB; TKG)
D16S53911,13 (AddexBio; ATCC; CLS; PubMed=25877200; PubMed=25926053; TKG)
11,12,13 (DSMZ)
D18S5111,13
D21S1129,31
D3S135815,17
D5S81811,15 (AddexBio; CLS; KCLB; PubMed=25877200; PubMed=25926053; TKG)
11,15,16 (ATCC)
11,14,15 (DSMZ)
D7S82010.3,11 (ATCC; DSMZ; PubMed=25877200)
11 (AddexBio; CLS; KCLB; PubMed=25926053)
10,10.3 (TKG)
D8S117912,16
FGA21,22
Penta D10
Penta E15,16
TH016,7
TPOX8,9
vWA15,17,18 (ATCC)
15,17 (AddexBio; CLS; KCLB; PubMed=11416159; PubMed=25877200; PubMed=25926053; TKG)
15,16,18 (DSMZ)
Web pages http://www.pawefish.path.cam.ac.uk/ColonCellLineDescriptions/LS174T.html
http://tcpaportal.org/mclp/
Publications

PubMed=1262041; DOI=10.1007/BF02796440
Tom B.H., Rutzky L.P., Jakstys M.M., Oyasu R., Kaye C.I., Kahan B.D.
Human colonic adenocarcinoma cells. I. Establishment and description of a new line.
In Vitro 12:180-191(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6652615; DOI=10.1016/0165-4608(83)90092-4
Chen T.-R., Hay R.J., Macy M.L.
Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins.
Cancer Genet. Cytogenet. 10:351-362(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3349466
Chantret I., Barbat A., Dussaulx E., Brattain M.G., Zweibaum A.
Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
Cancer Res. 48:1936-1942(1988)

PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B
Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.
Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.
Cancer 71:315-321(1993)

PubMed=8895552; DOI=10.1002/(SICI)1097-0215(19960927)68:1<126::AID-IJC22>3.0.CO;2-8
Suardet L., Li C., Little J.B.
Radio-induced modulation of transforming growth factor beta1 sensitivity in a p53 wild-type human colorectal-cancer cell line.
Int. J. Cancer 68:126-131(1996)

PubMed=9000147
Cottu P.H., Muzeau F., Estreicher A., Flejou J.-F., Iggo R.D., Thomas G., Hamelin R.
Inverse correlation between RER+ status and p53 mutation in colorectal cancer cell lines.
Oncogene 13:2727-2730(1996)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330
Ilyas M., Tomlinson I.P.M., Rowan A., Pignatelli M., Bodmer W.F.
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298
Abdel-Rahman W.M., Katsura K., Rens W., Gorman P.A., Sheer D., Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11526487; DOI=10.1038/sj.onc.1204611
Gayet J., Zhou X.-P., Duval A., Rolland S., Hoang J.-M., Cottu P.H., Hamelin R.
Extensive characterization of genetic alterations in a series of human colorectal cancer cell lines.
Oncogene 20:5025-5032(2001)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C., Shipley J., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=16854228; DOI=10.1186/1476-4598-5-29
Bandres E., Cubedo E., Agirre X., Malumbres R., Zarate R., Ramirez N., Abajo A., Navarro A., Moreno I., Monzo M., Garcia-Foncillas J.
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29-29(2006)

PubMed=18258742; DOI=10.1073/pnas.0712176105
Emaduddin M., Bicknell D.C., Bodmer W.F., Feller S.M.
Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.
Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008)

PubMed=19132987; DOI=10.1111/j.1600-0463.2008.01042.x
Li Y., Tanaka Y., Tada M., Hua R., Seto M., Asaoka Y., Ohta M., Kanai F., Yoshida H., Kawabe T., Sano T., Motojima T., Yokosuka O., Omata M.
Absence of the AKT1 pleckstrin homology domain mutation in Japanese gastrointestinal and liver cancer patients.
APMIS 116:931-933(2008)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=23272949; DOI=10.1186/1755-8794-5-66
Schlicker A., Beran G., Chresta C.M., McWalter G., Pritchard A., Weston S., Runswick S., Davenport S., Heathcote K., Castro D.A., Orphanides G., French T., Wessels L.F.A.
Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.
BMC Med. Genomics 5:66-66(2012)

PubMed=24042735; DOI=10.1038/oncsis.2013.35
Ahmed D., Eide P.W., Eilertsen I.A., Danielsen S.A., Eknaes M., Hektoen M., Lind G.E., Lothe R.A.
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:E71-E71(2013)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=24840470; DOI=10.1016/j.jprot.2014.05.002
Chik J.H.L., Zhou J., Moh E.S.X., Christopherson R., Clarke S.J., Molloy M.P., Packer N.H.
Comprehensive glycomics comparison between colon cancer cell cultures and tumours: implications for biomarker studies.
J. Proteomics 108:146-162(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=28683746; DOI=10.1186/s12943-017-0691-y
Berg K.C.G., Eide P.W., Eilertsen I.A., Johannessen B., Bruun J., Danielsen S.A., Bjornslett M., Meza-Zepeda L.A., Eknaes M., Lind G.E., Myklebost O., Skotheim R.I., Sveen A., Lothe R.A.
Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies.
Mol. Cancer 16:116-116(2017)

PubMed=29101300; DOI=10.15252/msb.20177701
Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.
Pharmacoproteomic characterisation of human colon and rectal cancer.
Mol. Syst. Biol. 13:951-951(2017)

Cross-references
Cell line collections AddexBio; C0009013/379
ATCC; CL-188
ATCC; CCL-188 - Discontinued
BCRC; 60053
CLS; 300392/p720_LS-174T
DSMZ; ACC-759
ECACC; 87060401
KCB; KCB 200829YJ
KCLB; 10188
NCBI_Iran; C568
RCB; RCB1961 - Discontinued
TKG; TKG 0406
Cell line databases/resources CLDB; cl3261
CCRID; 3111C0001CCC000248
CCRID; 3131C0001000700032
CCRID; 3142C0001000000129
ColonAtlas; LS174T
IGRhCellID; LS174T
LINCS_LDP; LCL-1182
Lonza; 1127
TOKU-E; 2235
Ontologies BTO; BTO:0001553
CLO; CLO_0007393
CLO; CLO_0007403
EFO; EFO_0002227
MCCL; MCC:0000504
Biological sample resources BioSample; SAMN03470826
BioSample; SAMN03471296
Chemistry resources ChEMBL-Cells; CHEMBL3307536
ChEMBL-Targets; CHEMBL614097
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM513911
GEO; GSM514297
GEO; GSM741264
GEO; GSM784021
GEO; GSM827345
GEO; GSM1346883
GEO; GSM1374632
GEO; GSM1374633
GEO; GSM1448164
GEO; GSM2550009
Metabolomic databases MetaboLights; MTBLS227
Other Wikidata; Q54903053
Polymorphism and mutation databases Cosmic; 711255
Cosmic; 719679
Cosmic; 724843
Cosmic; 738927
Cosmic; 873703
Cosmic; 875424
Cosmic; 876717
Cosmic; 887219
Cosmic; 889533
Cosmic; 907793
Cosmic; 934563
Cosmic; 948131
Cosmic; 948856
Cosmic; 985998
Cosmic; 995405
Cosmic; 1043822
Cosmic; 1057755
Cosmic; 1066210
Cosmic; 1122328
Cosmic; 1132565
Cosmic; 1132692
Cosmic; 1187310
Cosmic; 1184087
Cosmic; 1184334
Cosmic; 1312305
Cosmic; 1479573
Cosmic; 1486134
Cosmic; 1524341
Cosmic; 1676738
Cosmic; 1708419
Cosmic; 1805263
Cosmic; 2301546
Cosmic; 2301997
Cosmic; 2646768
Cosmic; 2651866
Cosmic; 2668256
Proteomic databases PRIDE; PXD005354