Cellosaurus M14 (CVCL_1395)

Cell line name M14
Synonyms M14-MEL; UCLA-SO-M14; UCLA SO M14; UCLA-SO-14; Melanoma 14
Accession CVCL_1395
Resource Identification Initiative To cite this cell line use: M14 (RRID:CVCL_1395)
Comments Part of: MD Anderson Cell Lines Project.
Part of: NCI60 cancer cell line panel.
Doubling time: 26.3 hours (NCI-DTP).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: Heterozygous for BRAF p.Val600Glu (PubMed=17088437).
Sequence variation: Heterozygous for TP53 p.Gly266Glu (PubMed=17088437; PubMed=18277095).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: DNA methylation analysis.
Omics: Fluorescence phenotype profiling.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Subcutaneous; right buttock.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_6279 (BHP 14-9); CVCL_6281 (BHP 17-10); CVCL_6284 (BHP 5-16); CVCL_A779 (M14-A); CVCL_0417 (MDA-MB-435); CVCL_0467 (NPA)
Originate from same individual CVCL_DI53 ! MF14
CVCL_DI50 ! ML14
Sex of cell Male
Category Cancer cell line
STR profile Source(s): Cosmic-CLP; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=28940260

Markers:
AmelogeninX
CSF1PO11
D13S31712
D16S5399,13
D18S5113,17
D19S43314,15
D21S1130
D2S133819,24
D3S135814,16
D5S81811,12
D7S8208,10
D8S117913
FGA21
TH016,7
TPOX8,11
vWA16,18
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=48
http://tcpaportal.org/mclp/
Publications

PubMed=954005
Zielske J.V., Golub S.H.
Fetal calf serum-induced blastogenic and cytotoxic responses of human lymphocytes.
Cancer Res. 36:3842-3846(1976)

PubMed=945229; DOI=10.1002/ijc.2910170408
Sulit H.L., Golub S.H., Irie R.F., Gupta R.K., Grooms G.A., Morton D.L.
Human tumor cells grown in fetal calf serum and human serum: influences on the tests for lymphocyte cytotoxicity, serum blocking and serum arming effects.
Int. J. Cancer 17:461-468(1976)

PubMed=1260767
Chee D.O., Boddie A.W., Roth J.A., Holmes E.C., Morton D.L.
Production of melanoma-associated antigen(s) by a defined malignant melanoma cell strain grown in chemically defined medium.
Cancer Res. 36:1503-1509(1976)

PubMed=204583; DOI=10.1002/ijc.2910210308
Saxton R.E., Irie R.F., Ferrone S., Pellegrino M.A., Morton D.L.
Establishment of paired tumor cells and autologous virus-transformed cell lines to define humoral immune responses in melanoma and sarcoma patients.
Int. J. Cancer 21:299-306(1978)

PubMed=6876798; DOI=10.1002/jso.2930230404
Chee D.O., Gupta R.K., Morton D.L.
Humoral response of melanoma patients to two different tumor-associated antigens.
J. Surg. Oncol. 23:228-235(1983)

PubMed=6470027; DOI=10.1007/BF00402467
Katano M., Saxton R.E., Cochran A.J., Irie R.F.
Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice.
J. Cancer Res. Clin. Oncol. 108:197-203(1984)

PubMed=3046746; DOI=10.1007/BF00200019
Wong J.H., Aguero B., Gupta R.K., Morton D.L.
Recovery of a cell surface fetal antigen from circulating immune complexes of melanoma patients.
Cancer Immunol. Immunother. 27:142-146(1988)

PubMed=1918136; DOI=10.1083/jcb.115.1.191
Quax P.H., van Muijen G.N.P., Weening-Verhoeff E.J., Lund L.R., Dano K., Ruiter D.J., Verheijen J.H.
Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation.
J. Cell Biol. 115:191-199(1991)

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K., Vistica D.T., Hose C., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J., Boyd M.
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

PubMed=2060184; DOI=10.1007/BF01753729
van Muijen G.N.P., Cornelissen L.M.H.A., Jansen C.F.J., Figdor C.G., Johnson J.P., Brocker E.-B., Ruiter D.J.
Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice.
Clin. Exp. Metastasis 9:259-272(1991)

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P., Iyer V., Jeffrey S.S., Van de Rijn M., Waltham M., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433
Ikediobi O.N., Davies H., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., Mironenko T., Parker A., Perry J., Raine K., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=18277095; DOI=10.4161/cbt.7.5.5712
Berglind H., Pawitan Y., Kato S., Ishioka C., Soussi T.
Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination.
Cancer Biol. Ther. 7:699-708(2008)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=19549886; DOI=10.1158/0008-5472.CAN-09-1528
Chambers A.F.
MDA-MB-435 and M14 cell lines: identical but not M14 melanoma?
Cancer Res. 69:5292-5293(2009)

PubMed=19727395; DOI=10.1371/journal.pone.0006888
Wadlow R.C., Wittner B.S., Finley S.A., Bergquist H., Upadhyay R., Finn S., Loda M., Mahmood U., Ramaswamy S.
Systems-level modeling of cancer-fibroblast interaction.
PLoS ONE 4:E6888-E6888(2009)

DOI=10.4172/2157-7145.S2-005
Fang R., Shewale J.G., Nguyen V.T., Cardoso H., Swerdel M., Hart R.P., Furtado M.R.
STR profiling of human cell lines: challenges and possible solutions to the growing problem.
J. Forensic Res. 2 Suppl. 2:5-5(2011)

PubMed=22384151; DOI=10.1371/journal.pone.0032096
Lee J.-S., Kim Y.K., Kim H.J., Hajar S., Tan Y.L., Kang N.-Y., Ng S.H., Yoon C.N., Chang Y.-T.
Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
PLoS ONE 7:E32096-E32096(2012)

PubMed=22628656; DOI=10.1126/science.1218595
Jain M., Nilsson R., Sharma S., Madhusudhan N., Kitami T., Souza A.L., Kafri R., Kirschner M.W., Clish C.B., Mootha V.K.
Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.
Science 336:1040-1044(2012)

PubMed=23856246; DOI=10.1158/0008-5472.CAN-12-3342
Abaan O.D., Polley E.C., Davis S.R., Zhu Y.J., Bilke S., Walker R.L., Pineda M., Gindin Y., Jiang Y., Reinhold W.C., Holbeck S.L., Simon R.M., Doroshow J.H., Pommier Y., Meltzer P.S.
The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
Cancer Res. 73:4372-4382(2013)

PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Moghaddas Gholami A., Hahne H., Wu Z., Auer F.J., Meng C., Wilhelm M., Kuster B.
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

PubMed=24279929; DOI=10.1186/2049-3002-1-20
Dolfi S.C., Chan L.L.-Y., Qiu J., Tedeschi P.M., Bertino J.R., Hirshfield K.M., Oltvai Z.N., Vazquez A.
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
Cancer Metab. 1:20-20(2013)

PubMed=24670534; DOI=10.1371/journal.pone.0092047
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

PubMed=27377824; DOI=10.1038/sdata.2016.52
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27807467; DOI=10.1186/s13100-016-0078-4
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=28940260; DOI=10.1002/ijc.31067
Korch C., Hall E.M., Dirks W.G., Ewing M., Faries M., Varella-Garcia M., Robinson S., Storts D., Turner J.A., Wang Y., Burnett E.C., Healy L., Kniss D., Neve R.M., Nims R.W., Reid Y.A., Robinson W.A., Capes-Davis A.
Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
Int. J. Cancer 0:0-0(2017)

Cross-references
Cell line collections NCI-DTP; M14
Cell line databases/resources Cosmic-CLP; 905975
GDSC; 905975
SKY/M-FISH/CGH; 2813
Ontologies BTO; BTO:0002805
EFO; EFO_0003056
Biological sample resources BioSample; SAMN06129842
Chemistry resources ChEMBL-Cells; CHEMBL3307591
ChEMBL-Targets; CHEMBL614317
Gene expression databases GEO; GSM2140
GEO; GSM50201
GEO; GSM50263
GEO; GSM188222
GEO; GSM188252
GEO; GSM188311
GEO; GSM750799
GEO; GSM799352
GEO; GSM799415
GEO; GSM847034
GEO; GSM844583
GEO; GSM1153422
GEO; GSM1181292
GEO; GSM1181336
GEO; GSM1670072
GEO; GSM2124668
Polymorphism and mutation databases Cosmic; 686479
Cosmic; 687445
Cosmic; 875889
Cosmic; 897467
Cosmic; 905975
Cosmic; 974068
Cosmic; 974261
Cosmic; 1022278
Cosmic; 1044265
Cosmic; 1092611
Cosmic; 1109808
Cosmic; 1132591
Cosmic; 1158815
Cosmic; 1295340
Cosmic; 1303040
Cosmic; 1305365
Cosmic; 1312348
Cosmic; 1459642
Cosmic; 1537485
Cosmic; 1888913
Cosmic; 1998453