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Cellosaurus NCI-H524 (CVCL_1568)

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Cell line name NCI-H524
Synonyms H524; H-524; NCIH524
Accession CVCL_1568
Resource Identification Initiative To cite this cell line use: NCI-H524 (RRID:CVCL_1568)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Doubling time: 100 hours (PubMed=25984343).
Microsatellite instability: Instable (MSI-low) (Sanger).
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for RB1 p.Ser576Ter (c.1727C>G) (ATCC).
Homozygous for TP53 p.Thr155Asn (c.464C>A) (ATCC).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.55
Native American0
East Asian, North1.32
East Asian, South0
South Asian0
European, North66.11
European, South32.01
Disease Small cell lung carcinoma (NCIt: C4917)
Derived from metastatic site: Lymph node.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_WM69 (NCI-H524-A)
Sex of cell Male
Age at sampling 63Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; Cosmic-CLP; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D2S133816,17
D3S135815
D5S81812
D7S82011,12
D8S117913,15
D13S31712
D16S53912
D18S5112,13
D19S43316
D21S1129,30
FGA21,22,25 (CCRID)
25 (PubMed=25877200)
Penta D12,13
Penta E5,15
TH018,9.3
TPOX8,10
vWA14,17

Run an STR similarity search on this cell line
Web pages http://tcpaportal.org/mclp/
http://www.cellresource.cn/fdetail.aspx?id=1545
Publications

PubMed=2985257
Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2985258
Gazdar A.F., Carney D.N., Nau M.M., Minna J.D.
Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.
Cancer Res. 45:2924-2930(1985)

PubMed=2473086
Broers J.L.V., Rot M.K., Oostendorp T., Bepler G., De Leij L., Carney D.N., Vooijs G.P., Ramaekers F.C.S.
Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines.
J. Cell Sci. 91:91-108(1988)

PubMed=1845952; DOI=10.1002/1097-0142(19910201)67:3<619::AID-CNCR2820670317>3.0.CO;2-Y
Broers J.L.V., Mijnheere E.P., Rot M.K., Schaart G., Sijlmans A., Boerman O.C., Ramaekers F.C.S.
Novel antigens characteristic of neuroendocrine malignancies.
Cancer 67:619-633(1991)

PubMed=1320893; DOI=10.1159/000163712
Verbeeck M.A.E., Elands J.P.M., de Leij L.F.M.H., Buys C.H.C.M., Carney D.N., Bepler G., Roebroeck A.J.M., van de Ven W.J.M., Burbach J.P.
Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines.
Pathobiology 60:136-142(1992)

PubMed=7720728
Senden N.H.M., van de Velde H.J.K., Broers J.L.V., Timmer E.D.J., Kuijpers H.J.H., Roebroek A.J.M., Van de Ven W.J.M., Ramaekers F.C.S.
Subcellular localization and supramolecular organization of neuroendocrine-specific protein B (NSP-B) in small cell lung cancer.
Eur. J. Cell Biol. 65:341-353(1994)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. 63 Suppl. 24:210-217(1996)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Modi S., Kubo A., Oie H., Coxon A.B., Rehmatulla A., Kaye F.J.
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=17426248; DOI=10.1158/1541-7786.MCR-06-0367
Olejniczak E.T., Van Sant C., Anderson M.G., Wang G., Tahir S.K., Sauter G., Lesniewski R., Semizarov D.
Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.
Mol. Cancer Res. 5:331-339(2007)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112
Byers L.A., Wang J., Nilsson M.B., Fujimoto J., Saintigny P., Yordy J., Giri U., Peyton M., Fan Y.H., Diao L., Masrorpour F., Shen L., Liu W., Duchemann B., Tumula P., Bhardwaj V., Welsh J., Weber S., Glisson B.S., Kalhor N., Wistuba I.I., Girard L., Lippman S.M., Mills G.B., Coombes K.R., Weinstein J.N., Minna J.D., Heymach J.V.
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections ATCC; CRL-5831
KCB; KCB 2010137YJ
KCLB; 90524 - Discontinued
Cell line databases/resources CCLE; NCIH524_LUNG
CCRID; 3111C0001CCC000356
Cell_Model_Passport; SIDM01129
Cosmic-CLP; 908483
DepMap; ACH-000816
GDSC; 908483
IGRhCellID; NCIH524
LINCS_LDP; LCL-1857
Ontologies BTO; BTO:0004825
CLO; CLO_0008095
EFO; EFO_0002296
Biological sample resources BioSample; SAMN03472976
BioSample; SAMN10988203
Chemistry resources ChEMBL-Cells; CHEMBL3308296
ChEMBL-Targets; CHEMBL2366342
PharmacoDB; NCIH524_1119_2019
Gene expression databases ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM169442
GEO; GSM170710
GEO; GSM170712
GEO; GSM794292
GEO; GSM845548
GEO; GSM887435
GEO; GSM888515
GEO; GSM1670249
Other Wikidata; Q54908075
Polymorphism and mutation databases Cosmic; 688024
Cosmic; 844555
Cosmic; 850442
Cosmic; 877208
Cosmic; 877358
Cosmic; 908483
Cosmic; 980933
Cosmic; 1032399
Cosmic; 1152472
Cosmic; 1759289
Cosmic; 1891904
Cosmic; 2125200
IARC_TP53; 21575
LiGeA; CCLE_825
Proteomic databases PRIDE; PXD011896
Entry history
Entry creation04-Apr-2012
Last entry updated06-Sep-2019
Version number27