Cellosaurus NCI-H69 (CVCL_1579)

Cell line name NCI-H69
Synonyms NCI-H-69; NCI H69; H69; H-69; NCIH69; NCI-HUT-69; H69/P; NCI-H69C; H69C; H69c
Accession CVCL_1579
Resource Identification Initiative To cite this cell line use: NCI-H69 (RRID:CVCL_1579)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Small cell lung carcinoma (NCIt: C4917)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_0B21 (H69/BCNU); CVCL_0B22 (H69/DAU); CVCL_X029 (H69/Txl); CVCL_AZ78 (H69/VDS); CVCL_0B23 (H69/VP); CVCL_3513 (H69AR); CVCL_2466 (H69V); CVCL_S830 (H69VZ); CVCL_A537 (NCI-H249); CVCL_2137 (NCI-H69/CPR); CVCL_2138 (NCI-H69/LX10); CVCL_2139 (NCI-H69/LX20); CVCL_2140 (NCI-H69/LX4); CVCL_3058 (NCI-H69VCR/R); CVCL_A590 (NCI-N592)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; ECACC; KCLB; PubMed=25877200

Markers:
AmelogeninX,Y (ATCC; Cosmic-CLP; ECACC; KCLB; PubMed=25877200)
X (CLS)
CSF1PO10,12 (ATCC; CLS; ECACC; KCLB)
10,13 (Cosmic-CLP; PubMed=25877200)
D13S31712
D16S53911
D18S5112 (CLS)
12,15 (PubMed=25877200)
D21S1130,31.2
D3S135816
D5S81811,13
D7S8209
D8S117913
FGA24
Penta D9,11
Penta E12
TH018,9
TPOX10
vWA17 (Cosmic-CLP; PubMed=25877200)
16,17 (ATCC; CLS; ECACC; KCLB)
Web pages http://tcpaportal.org/mclp/
Publications

PubMed=6108156
Gazdar A.F., Carney D.N., Russell E.K., Sims H.L., Baylin S.B., Bunn P.A. Jr., Guccion J.G., Minna J.D.
Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties.
Cancer Res. 40:3502-3507(1980)

PubMed=6148444; DOI=10.1093/jnci/73.4.801
Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H., Mitchell J.B.
Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=2985257
Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2985258
Gazdar A.F., Carney D.N., Nau M.M., Minna J.D.
Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.
Cancer Res. 45:2924-2930(1985)

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4
Whang-Peng J., Lee E.C.
Cytogenetics of human small cell lung cancer.
Recent Results Cancer Res. 97:37-46(1985)

PubMed=3034934; DOI=10.1002/jcb.240330405
Ibson J.M., Waters J.J., Twentyman P.R., Bleehen N.M., Rabbitts P.H.
Oncogene amplification and chromosomal abnormalities in small cell lung cancer.
J. Cell. Biochem. 33:267-288(1987)

PubMed=2574988; DOI=10.1038/bjc.1989.376
Jensen P.B., Vindelov L.L., Roed H., Demant E.J.F., Sehested M., Skovsgaard T., Hansen H.H.
In vitro evaluation of the potential of aclarubicin in the treatment of small cell carcinoma of the lung (SCCL).
Br. J. Cancer 60:838-844(1989)

PubMed=7736387; DOI=10.1002/1097-0142(19950515)75:10<2442::AID-CNCR2820751009>3.0.CO;2-Q
Campling B.G., Sarda I.R., Baer K.A., Pang S.C., Baker H.M., Lofters W.S., Flynn T.G.
Secretion of atrial natriuretic peptide and vasopressin by small cell lung cancer.
Cancer 75:2442-2451(1995)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477
Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.
Insulin-like growth factor expression in human cancer cell lines.
J. Biol. Chem. 271:11477-11483(1996)

PubMed=8712684
Khan M.Z., McNicol A.M., Freshney R.I.
Tumorigenicity, invasion and metastasis of the small cell lung cancer cell line NCI-H69 and two derivative lines MOG-H69V and MOG-H69VZ.
Anticancer Res. 16:1659-1669(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. 63 Suppl. 24:210-217(1996)

PubMed=9212023; DOI=10.1016/S0360-3016(97)00245-9
Krarup M., Poulsen H.S., Spang-Thomsen M.
Cellular radiosensitivity of small-cell lung cancer cell lines.
Int. J. Radiat. Oncol. Biol. Phys. 38:191-196(1997)

PubMed=9815546
Campling B.G., Young L.C., Baer K.A., Lam Y.-M., Deeley R.G., Cole S.P.C., Gerlach J.H.
Expression of the MRP and MDR1 multidrug resistance genes in small cell lung cancer.
Clin. Cancer Res. 3:115-122(1997)

PubMed=9559344; DOI=10.1002/(SICI)1098-2264(199804)21:4<326::AID-GCC6>3.0.CO;2-0
Mulligan L.M., Timmer T., Ivanchuk S.M., Campling B.G., Young L.C., Rabbitts P.H., Sundaresan V., Hofstra R.M., Eng C.
Investigation of the genes for RET and its ligand complex, GDNF/GFR alpha-I, in small cell lung carcinoma.
Genes Chromosomes Cancer 21:326-332(1998)

PubMed=9744504; DOI=10.1038/bjc.1998.553
Damstrup L., Rude Voldborg B., Spang-Thomsen M., Brunner N., Skovgaard Poulsen H.
In vitro invasion of small-cell lung cancer cell lines correlates with expression of epidermal growth factor receptor.
Br. J. Cancer 78:631-640(1998)

PubMed=10037197
Tang X.X., Brodeur G.M., Campling B.G., Ikegaki N.
Coexpression of transcripts encoding EPHB receptor protein tyrosine kinases and their ephrin-B ligands in human small cell lung carcinoma.
Clin. Cancer Res. 5:455-460(1999)

PubMed=11005564; DOI=10.1038/sj.neo.7900094
Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.
Mutation and expression of the DCC gene in human lung cancer.
Neoplasia 2:300-305(2000)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Modi S., Kubo A., Oie H., Coxon A.B., Rehmatulla A., Kaye F.J.
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=12569360; DOI=10.1038/sj.onc.1206106
Funakoshi T., Tachibana I., Hoshida Y., Kimura H., Takeda Y., Kijima T., Nishino K., Goto H., Yoneda T., Kumagai T., Osaki T., Hayashi S., Aozasa K., Kawase I.
Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer.
Oncogene 22:674-687(2003)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections ATCC; HTB-119
CLS; 300185/p530_NCI-H69
ECACC; 91091802
IZSLER; BS TCL 172
KCLB; 30119
NCI-DTP; NCI-H69
Cell line databases/resources CLDB; cl1552
CCLE; NCIH69_LUNG
CCRID; 3111C0001CCC000358
Cosmic-CLP; 688027
GDSC; 688027
IGRhCellID; NCIH69
LINCS_LDP; LCL-1859
Ontologies BTO; BTO:0003022
CLO; CLO_0003609
CLO; CLO_0008105
EFO; EFO_0002300
Biological sample resources BioSample; SAMN03473085
Chemistry resources ChEMBL-Cells; CHEMBL3307712
ChEMBL-Targets; CHEMBL614805
Gene expression databases GEO; GSM169443
GEO; GSM380498
GEO; GSM536334
GEO; GSM784197
GEO; GSM794269
GEO; GSM827305
GEO; GSM845550
GEO; GSM844659
GEO; GSM887442
GEO; GSM888522
GEO; GSM1670258
Other Wikidata; Q38568854
Polymorphism and mutation databases Cosmic; 688027
Cosmic; 713879
Cosmic; 755459
Cosmic; 801610
Cosmic; 808518
Cosmic; 844545
Cosmic; 844817
Cosmic; 876167
Cosmic; 877351
Cosmic; 918010
Cosmic; 929160
Cosmic; 1006522
Cosmic; 1021849
Cosmic; 1152461
Cosmic; 1175859
Cosmic; 1212189
Cosmic; 1219096
Cosmic; 1239934
Cosmic; 1434964
Cosmic; 1571755
Cosmic; 1609516
Cosmic; 1995588
Cosmic; 1998272
Cosmic; 2125215
Cosmic; 2239080