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Cellosaurus CCRF-HSB-2 (CVCL_1859)

[Text version]
Cell line name CCRF-HSB-2
Synonyms CCRF HSB-2; CCRF-HSB2; HSB-2; HSB.2; HSB2; H-SB2; SB-2; HSB; H.S.B.; GM04155
Accession CVCL_1859
Secondary accession CVCL_5938
Resource Identification Initiative To cite this cell line use: CCRF-HSB-2 (RRID:CVCL_1859)
Comments Problematic cell line: Partially contaminated. The stock formerly distributed by CLS under catalog number 300214 was contaminated. Compared to the STR values obtained by other distributors it differed from having: Amelogenin: X; CSF1PO: 8,12; D16S539: 9,13,14; D18S51: 9,13,14; D21S11: 28,29; D7S820: 10,14; D8S1179: 9,15; FGA: 22,23,24; Penta D: 9; Penta E: 6,13 and TH01: 8,10.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Characteristics: Established by serial transplantation into newborn Syrian hamsters.
Doubling time: 40 hours (PubMed=17117183); ~25 hours (DSMZ=ACC-435); 24 hours (Note=Lot 042091) (JCRB=JCRB0031).
Microsatellite instability: Instable (MSI) (PubMed=10739008; PubMed=11226526).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Transcriptome analysis by RNAseq.
Misspelling: CCRF-HBS-2; NCBI_Iran=C530.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; 10879; STIL + HGNC; 11556; TAL1; Name(s)=STIL-TAL1, SIL-TAL1, SIL-SCL (PubMed=2255914).
  • Mutation; HGNC; 3430; ERBB2; Simple; p.Pro1175Arg (c.3524C>G); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 3690; FGFR3; Simple; p.Tyr226His (c.676T>C); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 3690; FGFR3; Simple; p.Arg621His (c.1862G>A); ClinVar=VCV000016355; Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Leu1600Pro (c.4799T>C); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000040468; Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 8979; PIK3R1; Simple; p.Thr454Ile (c.1361C>T); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 8979; PIK3R1; Simple; p.Gln455Lys (c.1363C>A); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=2144611).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A0DR (HSB.2-A7-D2)CVCL_A0DS (HSB.2-A7-D9)CVCL_A0DT (HSB.2-C5-B2)
CVCL_1Y12 (HSB2/GS)
Originate from same individual CVCL_1860 ! CCRF-SB
Sex of cell Male
Age at sampling 11Y
Category Cancer cell line
STR profile Source(s): ATCC; DSMZ; ECACC; JCRB; KCLB; RCB

Markers:
AmelogeninX,Y
CSF1PO10,11 (DSMZ)
10,12 (ATCC; ECACC; JCRB; KCLB; RCB)
D2S133817,18
D3S135814,16
D5S81811,12
D7S82011,12
D8S117910,15
D13S31710,12
D16S5399,12 (DSMZ)
9,13 (ATCC; ECACC; JCRB; RCB)
D18S519,14
D19S43314,20
D21S1127,28
FGA24,25
Penta D9,12
Penta E8,13
TH019,10 (ATCC; DSMZ; ECACC; JCRB; RCB)
9,9.3 (KCLB)
TPOX8
vWA18,19 (ATCC; DSMZ; ECACC; JCRB; KCLB)
18,19,20 (RCB)

Run an STR similarity search on this cell line
Publications

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4991454; DOI=10.1016/0014-4827(79)90504-4
Adams R.A., Pothier L., Flowers A., Lazarus H., Farber S., Foley G.E.
The question of stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia.
Exp. Cell Res. 62:5-10(1970)

PubMed=4363409; DOI=10.1084/jem.139.5.1070
Kaplan J., Shope T.C., Peterson W.D. Jr.
Epstein-Barr virus-negative human malignant T-cell lines.
J. Exp. Med. 139:1070-1076(1974)

PubMed=4523807; DOI=10.1007/BF02616012
Lazarus H., Barell E.F., Oppenheim S.O., Krishan A.
Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood.
In Vitro 9:303-310(1974)

PubMed=62390; DOI=10.1111/j.1365-3083.1976.tb03029.x
Yount W.J., Utsinger P.D., Hutt L.M., Buchanan P.D., Korn J.H., Fuller C.R., Logue M., Pagano J.S.
Subpopulations of human lymphoblastoid cell lines. Correlation with the expression of surface receptors and content of Epstein-Barr virus genome.
Scand. J. Immunol. 5:795-810(1976)

PubMed=1086134
Kaplan J., Peterson W.D. Jr.
Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.
Cancer Res. 36:3471-3475(1976)

PubMed=6600440; DOI=10.1007/BF02617996
Uittenbogaart C.H., Cantor Y., Fahey J.L.
Growth of human malignant lymphoid cell lines in serum-free medium.
In Vitro 19:67-72(1983)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3495441; DOI=10.1002/eji.1830170503
Kanowith-Klein S., Saxon A., Uittenbogaart C.H.
Constitutive production of B cell differentiation factor-like activity by human T and B cell lines.
Eur. J. Immunol. 17:593-598(1987)

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x
Nakano A., Harada T., Morikawa S., Kato Y.
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Acta Pathol. Jpn. 40:107-115(1990)

PubMed=2144611; DOI=10.1128/mcb.10.10.5502-5509.1990
Cheng J., Haas M.
Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines.
Mol. Cell. Biol. 10:5502-5509(1990)

PubMed=2255914; DOI=10.1126/science.2255914
Aplan P.D., Lombardi D.P., Ginsberg A.M., Cossman J., Bertness V.L., Kirsch I.R.
Disruption of the human SCL locus by 'illegitimate' V-(D)-J recombinase activity.
Science 250:1426-1429(1990)

CLPUB00447
Mulivor R.A., Suchy S.F.
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7630190
Zhou M.-X., Gu L.-B., James C.D., He J., Yeager A.M., Smith S.D., Findley H.W.
Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia.
Leukemia 9:1159-1161(1995)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

DOI=10.11418/jtca1981.15.4_211
Matsuo Y., Okochi A., Ariyasu T., Iimura E., Ohno T.
Identification of cell lines with variable numbers of tandem repeat (VNTR) amplified by polymerase chain reaction.
Tissue Cult. Res. Commun. 15:211-219(1996)

PubMed=8558913
Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.
Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.
Leukemia 10:102-105(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x
Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N., Liu C., Kondo E., Minowada J., Akagi T.
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9787181; DOI=10.1182/blood.V92.9.3410
Sakai A., Thieblemont C., Wellmann A., Jaffe E.S., Raffeld M.
PTEN gene alterations in lymphoid neoplasms.
Blood 92:3410-3415(1998)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Burger R., Hansen-Hagge T.E., Drexler H.G., Gramatzki M.
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000),255-262.
Leuk. Res. 25:275-278(2001)

PubMed=14504097; DOI=10.1182/blood-2003-02-0418
Taketani T., Taki T., Sugita K., Furuichi Y., Ishii E., Hanada R., Tsuchida M., Sugita K., Ida K., Hayashi Y.
FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.
Blood 103:1085-1088(2004)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. IV, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=17117183; DOI=10.1038/sj.bjc.6603447
Beesley A.H., Palmer M.-L., Ford J., Weller R.E., Cummings A.J., Freitas J.R., Firth M.J., Perera K.U., de Klerk N.H., Kees U.R.
Authenticity and drug resistance in a panel of acute lymphoblastic leukaemia cell lines.
Br. J. Cancer 95:1537-1544(2006)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Sandberg Y., Verhaaf B., van Gastel-Mol E.J., Wolvers-Tettero I.L.M., de Vos J., MacLeod R.A.F., Noordzij J.G., Dik W.A., van Dongen J.J.M., Langerak A.W.
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=22675565; DOI=10.1371/journal.pone.0038463
Atak Z.K., De Keersmaecker K., Gianfelici V., Geerdens E., Vandepoel R., Pauwels D., Porcu M., Lahortiga I., Brys V., Dirks W.G., Quentmeier H., Cloos J., Cuppens H., Uyttebroeck A., Vandenberghe P., Cools J., Aerts S.
High accuracy mutation detection in leukemia on a selected panel of cancer genes.
PLoS ONE 7:E38463-E38463(2012)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5
Leo I.R., Aswad L., Stahl M., Kunold E., Post F., Erkers T., Struyf N., Mermelekas G., Joshi R.N., Gracia-Villacampa E., Ostling P., Kallioniemi O.-P., Pokrovskaja Tamm K., Siavelis I., Lehtio J., Vesterlund M., Jafari R.
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

Cross-references
Cell line collections (Providers) ATCC; CCL-120.1
CLS; 300214 - Discontinued
Coriell; GM04155
DSMZ; ACC-435
ECACC; 85112801
HIVReagentProgram; ARP-497
IZSLER; BS TCL 185
JCRB; JCRB0031
KCLB; 10120.1
NCBI_Iran; C530
RCB; RCB0016
Cell line databases/resources CLO; CLO_0002335
CLO; CLO_0004258
CLO; CLO_0016177
CLO; CLO_0050971
CLDB; cl678
cancercelllines; CVCL_1859
Cell_Model_Passport; SIDM01558
DSMZCellDive; ACC-435
LINCS_LDP; LCL-1914
Anatomy/cell type resources BTO; BTO:0005370
Biological sample resources BioSample; SAMN03472334
BioSample; SAMN03472701
BioSample; SAMN03473542
Chemistry resources ChEMBL-Cells; CHEMBL3307642
ChEMBL-Targets; CHEMBL614541
PubChem_Cell_line; CVCL_1859
Encyclopedic resources Wikidata; Q54809037
Gene expression databases GEO; GSM5137752
Polymorphism and mutation databases Cosmic; 683532
Cosmic; 801729
Cosmic; 850195
Cosmic; 913416
Cosmic; 922679
Cosmic; 932768
Cosmic; 996307
Cosmic; 998723
Cosmic; 1037688
Cosmic; 1115591
Cosmic; 1175128
Cosmic; 1226856
Cosmic; 1281369
Cosmic; 1330497
Cosmic; 1524797
Cosmic; 1641390
Cosmic; 1760523
Cosmic; 2165712
Cosmic; 2361365
Cosmic; 2458762
Cosmic; 2462861
Cosmic; 2602915
Cosmic; 2649236
IARC_TP53; 21649
Proteomic databases PRIDE; PXD023662
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number40