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Cellosaurus COLO 677 (CVCL_1862)

[Text version]
Cell line name COLO 677
Synonyms COLO-677; COLO #677; COLO677; Colorado 677
Accession CVCL_1862
Resource Identification Initiative To cite this cell line use: COLO 677 (RRID:CVCL_1862)
Comments Problematic cell line: Contaminated. Shown to be a RPMI-8226 derivative (PubMed=20143388). Originally thought to originate from the lymph nodes of a 50 year old male patient with a small cell lung carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00299.
Population: African American.
Characteristics: Produces IgG lambda (from parent cell line).
Doubling time: ~40 hours (DSMZ=ACC-248).
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: B-cell; CL=CL_0000236.
Sequence variations
  • Gene fusion; HGNC; 5722; IGKJ4 + HGNC; 5783; IGKV2-28; Name(s)=IGKV2-28-IGKJ4 (CelloPub=CLPUB00604).
  • Gene fusion; HGNC; 5865; IGLJ3 + HGNC; 5888; IGLV2-14; Name(s)=IGLV2-14-IGLJ3 (CelloPub=CLPUB00604).
  • Mutation; HGNC; 3236; EGFR; Simple; p.Thr751Ile (c.2252C>T); ClinVar=VCV000177773; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 12033; TRAF3; Simple; p.Lys191Leufs*60; Zygosity=Unspecified (from parent cell line).
Disease Plasma cell myeloma (NCIt: C3242)
Multiple myeloma (ORDO: Orphanet_29073)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0014 (RPMI-8226)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
STR profile Source(s): DSMZ

Markers:
AmelogeninX,Y
CSF1PO12
D2S133820
D3S135816,17
D5S81811,13
D7S8209,10
D8S117913
D13S31711
D16S5399
D18S5115,19
D19S43314
D21S1128,29
FGA19
Penta D3,11
Penta E16,17
TH018
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
https://www.keatslab.org/myeloma-cell-lines/hmcl-characteristics
Publications

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

CLPUB00604
Chow S.
Targeted capture and sequencing of immunoglobulin rearrangements in multiple myeloma to enable detection of minimal residual disease.
Thesis MSc (2017), University of Toronto, Canada

PubMed=32123307; DOI=10.1038/s41375-020-0785-1
Sarin V., Yu K., Ferguson I.D., Gugliemini O., Nix M.A., Hann B., Sirota M., Wiita A.P.
Evaluating the efficacy of multiple myeloma cell lines as models for patient tumors via transcriptomic correlation analysis.
Leukemia 34:2754-2765(2020)

Cross-references
Cell line collections (Providers) DSMZ; ACC-248 - Discontinued
ECACC; 93072109
Cell line databases/resources CLO; CLO_0002550
CLDB; cl837
CLDB; cl838
cancercelllines; CVCL_1862
DSMZCellDive; ACC-248
Biological sample resources BioSample; SAMN03151910
Chemistry resources PharmacoDB; COLO677_225_2019
Encyclopedic resources Wikidata; Q54814130
Gene expression databases GEO; GSM784242
Polymorphism and mutation databases Cosmic; 753542
IARC_TP53; 21233
Progenetix; CVCL_1862
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number32