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Cellosaurus COLO 677 (CVCL_1862)

[Text version]

Cell line name COLO 677
Synonyms COLO-677; COLO #677; COLO677; Colorado 677
Accession CVCL_1862
Resource Identification Initiative To cite this cell line use: COLO 677 (RRID:CVCL_1862)
Comments Problematic cell line: Contaminated. Shown to be a RPMI-8226 derivative (PubMed=20143388). Originally thought to originate from the lymph nodes of a 50 year-old male patient with a small cell lung carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00299.
Characteristics: Produces IgG lambda (from parent cell line).
Doubling time: ~40 hours (DSMZ).
Derived from sampling site: Peripheral blood.
Sequence variations Gene fusion; HGNC; 5722; IGKJ4 + HGNC; 5783; IGKV2-28; Name(s)=IGKV2-28-IGKJ4 (CelloPub=CLPUB00604).
Gene fusion; HGNC; 5865; IGLJ3 + HGNC; 5888; IGLV2-14; Name(s)=IGLV2-14-IGLJ3 (CelloPub=CLPUB00604).
Mutation; HGNC; 3236; EGFR; Simple; p.Thr751Ile (c.2252C>T); ClinVar=VCV000177773; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 12033; TRAF3; Simple; p.Lys191Leufs*60; Zygosity=Unspecified (from parent cell line).
Disease Plasma cell myeloma (NCIt: C3242)
Multiple myeloma (ORDO: Orphanet_29073)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0014 (RPMI-8226)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
STR profile Source(s): DSMZ

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S8209,10
D13S31711
D16S5399
TH018
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages http://iclac.org/wp-content/uploads/Cross-Contaminations_v11_distribution.xlsx
https://www.keatslab.org/myeloma-cell-lines/hmcl-characteristics
Publications

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

CLPUB00604
Chow S.
Targeted capture and sequencing of immunoglobulin rearrangements in multiple myeloma to enable detection of minimal residual disease.
Thesis MSc (2017), University of Toronto, Canada

Cross-references
Cell line collections DSMZ; ACC-248
ECACC; 93072109
Cell line databases/resources CLDB; cl837
CLDB; cl838
Ontologies CLO; CLO_0002550
Biological sample resources BioSample; SAMN03151910
Chemistry resources PharmacoDB; COLO677_225_2019
Gene expression databases GEO; GSM784242
Other Wikidata; Q54814130
Polymorphism and mutation databases Cosmic; 753542
IARC_TP53; 21233
Progenetix; CVCL_1862
Entry history
Entry creation04-Apr-2012
Last entry update23-Sep-2021
Version number26