Home  |  Contact

Cellosaurus HEp-2 (CVCL_1906)

[Text version]

Cell line name HEp-2
Synonyms Hep-2; HEP-2; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2
Accession CVCL_1906
Resource Identification Initiative To cite this cell line use: HEp-2 (RRID:CVCL_1906)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to originate from a 56 year old male patient with a laryngeal carcinoma.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00007.
Population: African American.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Deep RNAseq analysis.
Omics: Genome sequenced (low read coverage).
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Omics: Ubiquitination analysis by proteomics.
Derived from sampling site: Uterus; cervix.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_WH94 (Ban)CVCL_W888 (DRHEp2)CVCL_RX04 (HEp L1D7)
CVCL_RX05 (HEp L1G5)CVCL_2817 (HEp-2 clone 2B)CVCL_GZ00 (Hep-2 TPFR)
CVCL_2940 (HEp-2C)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CCRID; CLS; KCLB; PubMed=11416159; PubMed=17254797; RCB; TKG

D13S31712 (RCB)
12,13.3 (AddexBio; ATCC; CCRID; CLS; KCLB; PubMed=11416159; TKG)
12,14 (PubMed=17254797)
D18S5115 (PubMed=17254797)
16 (CCRID; CLS; PubMed=11416159)
D21S1126,27 (PubMed=17254797)
27,28 (CCRID; CLS; PubMed=11416159)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-204.html

Toolan H.W.
Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3 and H.Emb.Rh. No. 1.
Cancer Res. 14:660-666(1954)

Moore A.E., Sabachewsky L., Toolan H.W.
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res. 15:598-602(1955)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)

Ogura H., Yoshinouchi M., Kudo T., Imura M., Fujiwara T., Yabe Y.
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

Tsuboi I., Taguchi M., Kenmotsu M., Ban F., Machida K., Inagaki K., Ishi A., Tazawa T.
A new substrain derived from HEp-2 for the detection of anti-nuclear antibodies.
Tissue Cult. Res. Commun. 17:95-100(1998)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Azari S., Ahmadi N., Tehrani M.J., Shokri F.
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A.
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=29967540; DOI=10.1038/s41594-018-0084-y
Akimov V., Barrio-Hernandez I., Hansen S.V.F., Hallenborg P., Pedersen A.-K., Bekker-Jensen D.B., Puglia M., Christensen S.D.K., Vanselow J.T., Nielsen M.M., Kratchmarova I., Kelstrup C.D., Olsen J.V., Blagoev B.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.
Nat. Struct. Mol. Biol. 25:631-640(2018)

Gorphe P.
A comprehensive review of Hep-2 cell line in translational research for laryngeal cancer.
Am. J. Cancer Res. 9:644-649(2019)

Cell line collections AddexBio; C0050001/4983
BCRC; 60042 - Discontinued
BCRJ; 0101
CLS; 300397/p694_Hep-2
ECACC; 86030501
KCLB; 10023
NCBI_Iran; C144
RCB; RCB1889
TKG; TKG 0403
Cell line databases/resources CLDB; cl1646
CLDB; cl1647
CLDB; cl1649
CLDB; cl1650
CLDB; cl1653
CLDB; cl1654
CLDB; cl1655
CCRID; 3111C0001CCC000068
CCRID; 3111C0002000000056
CCRID; 3131C0001000700021
CCRID; 3142C0001000000004
CCRID; 3142C0001000000614
Cell_Model_Passport; SIDM01208
TOKU-E; 1442
Ontologies BTO; BTO:0000976
CLO; CLO_0003706
CLO; CLO_0003707
CLO; CLO_0050927
EFO; EFO_0006438
Biological sample resources BioSample; SAMN03151706
Chemistry resources ChEMBL-Cells; CHEMBL3307694
ChEMBL-Targets; CHEMBL614735
PharmacoDB; HEp2_540_2019
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM1374529
GEO; GSM1374530
Other Wikidata; Q16554737
Polymorphism and mutation databases Cosmic; 1339928
Cosmic; 1530739
Cosmic; 1969495
Cosmic; 2301559
Proteomic databases PRIDE; PXD006201
PRIDE; PXD013072
PRIDE; PXD014913
Entry history
Entry creation04-Apr-2012
Last entry update29-Oct-2020
Version number31