Cellosaurus MDA-N (CVCL_1910)

Cell line name MDA-N
Synonyms MDAN
Accession CVCL_1910
Resource Identification Initiative To cite this cell line use: MDA-N (RRID:CVCL_1910)
Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative (PubMed=20143388).
Part of: NCI60 cancer cell line panel.
Doubling time: 22.5 hours (NCI-DTP).
Microsatellite instability: Stable (MSS) (PubMed=12661003).
Sequence variation: Heterozygous for BRAF p.Val600Glu (from parent cell line).
Sequence variation: Heterozygous for TP53 p.Gly266Glu (from parent cell line).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Transcriptome analysis.
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Subcutaneous; right buttock.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0417 (MDA-MB-435)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): PubMed=19372543

Markers:
AmelogeninX
CSF1PO11
D13S31712
D16S53913
D18S5113,17
D19S43314
D21S1130
D2S133819,24
D3S135814
D5S81811,12
D7S8208,10
D8S117913
FGA21
TH016,7
TPOX8,11
vWA16,18
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://dtp.cancer.gov/discovery_development/nci-60/mda-mb-435.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=52
http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf
Publications

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K., Vistica D.T., Hose C., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J., Boyd M.
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P., Iyer V., Jeffrey S.S., Van de Rijn M., Waltham M., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.-F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=24670534; DOI=10.1371/journal.pone.0092047
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

Cross-references
Biological sample resources BioSample; SAMN03151833
Chemistry resources ChEMBL-Cells; CHEMBL3307532
ChEMBL-Targets; CHEMBL614078
Gene expression databases GEO; GSM2096
GEO; GSM750823
GEO; GSM799355
GEO; GSM799418
GEO; GSM1153425
GEO; GSM1181357
GEO; GSM1181358
Polymorphism and mutation databases Cosmic; 875880
Cosmic; 1998491