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Cellosaurus Sf-9ET (CVCL_1D71)

[Text version]

Cell line name Sf-9ET
Synonyms SF-9ET; Sf-9 Easy Titer; Sf-9 ET; Sf9-ET; Sf9-Easy Titer; Sf9 Easy Titer
Accession CVCL_1D71
Resource Identification Initiative To cite this cell line use: Sf-9ET (RRID:CVCL_1D71)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: The expression of the eGFP gene is under the control of the baculovirus polyhedrin promoter.
Doubling time: 28-32 hours (ATCC).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from sampling site: Ovary.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Parent: CVCL_0549 (Sf9)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line
Web pages http://www.ott.nih.gov/erma/development-sf-9et-easy-titer-inset-determining-baculovirus-titers
Publications

PubMed=19852765; DOI=10.2144/000113238
Hopkins R.F. III, Esposito D.
A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line.
BioTechniques 47:785-788(2009)

Cross-references
Cell line collections ATCC; CRL-3357
Kerafast; ENH022-FP
Other Wikidata; Q54952876
Entry history
Entry creation08-Jul-2015
Last entry update02-Jul-2020
Version number7