ID   Sup-T1 Nef-ER 31
AC   CVCL_1H35
SY   Nef-ER 31; Nef-ER #31; Nef-ER #31 Clone; Nef-ER Expressing Sup-T1 Cells (Clone 31)
DR   cancercelllines; CVCL_1H35
DR   HIVReagentProgram; ARP-6453
DR   Wikidata; Q54930388
RX   PubMed=11134296;
CC   Population: Caucasian.
CC   Characteristics: Expresses a chimeric Nef-estrogen receptor hormone-binding domain (Nef-ER) protein. Nef-ER is kept in an inactive state due to steric hindrance, and addition of the membrane-permeable drug 4-hydroxytamoxifen (4-HT), which binds to the ER domain, leads to inducible activation of Nef-ER within cells (PubMed=11134296).
CC   Sequence variation: Mutation; HGNC; 3236; EGFR; Simple; p.Cys251Tyr (c.752G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6342; KIT; Simple; p.Arg177His (c.530G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7029; MET; Simple; p.Arg191Trp (c.571C>T); ClinVar=VCV000568557; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Asp (c.1635G>T); ClinVar=VCV000217293; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu970Lys (c.2908G>A); ClinVar=VCV001057635; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 9585; PTCH1; Simple; p.Arg682Cys (c.2044C>T); ClinVar=VCV000237466; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 15533; SPRY4; Simple; p.Arg25Trp (c.73C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg267Leu (c.800G>T); ClinVar=VCV000852206; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: MGI; MGI:1352467; Esr1 (with p.Met1_Asp484del and p.Ile486_Ile599del).
CC   Transfected with: UniProtKB; Q90VU7; HIV-1 isolate NL4-3 nef.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
CC   Cell type: T-cell; CL=CL_0000084.
DI   NCIt; C7210; Childhood T lymphoblastic lymphoma
DI   ORDO; Orphanet_171918; T-cell non-Hodgkin lymphoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1714 ! SUP-T1
SX   Male
AG   8Y
CA   Cancer cell line
DT   Created: 08-07-15; Last updated: 05-10-23; Version: 14
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RX   PubMed=11134296; DOI=10.1128/JVI.75.2.834-843.2001;
RA   Walk S.F., Alexander M., Maier B., Hammarskjold M.-L., Rekosh D.M.,
RA   Ravichandran K.S.;
RT   "Design and use of an inducibly activated human immunodeficiency virus
RT   type 1 Nef to study immune modulation.";
RL   J. Virol. 75:834-843(2001).
//