Cellosaurus ECV-304 (CVCL_2029)

Cell line name ECV-304
Synonyms ECV 304; ECV304; ECV; E304; T24(ECV304)
Accession CVCL_2029
Resource Identification Initiative To cite this cell line use: ECV-304 (RRID:CVCL_2029)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (PubMed=10508494, PubMed=12197766, PubMed=20143388). Was originally thought to originate from endothelial cells obtained from the umbilical vein of a healthy donor.
Doubling time: ~2 days (DSMZ).
Omics: Deep tyrosine phosphoproteome analysis.
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
Children: CVCL_KS70 (CellSensor IL-1-bla ECV304); CVCL_J427 (t-HUE2); CVCL_J428 (t-HUE4)
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC; CLS; DSMZ; ECACC; JCRB; PubMed=11416159

Markers:
AmelogeninX
CSF1PO12
D13S31712
D16S5399
D18S5116,18
D21S1129
D3S135816
D5S81810
D7S82010,11
D8S117914
FGA17,22
Penta D11,15
Penta E7,10
TH016
TPOX8,11
vWA17
Web pages http://www.atcc.org/support/faqs/d0b4d/ECV304%20%20ATCC%20CRL1998-1057.aspx
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-2.html
http://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf
Publications

PubMed=1690702; DOI=10.1007/BF02624456
Takahashi K., Sawasaki Y., Hata J., Mukai K., Goto T.
Spontaneous transformation and immortalization of human endothelial cells.
In Vitro Cell. Dev. Biol. 26:265-274(1990)

PubMed=1378828; DOI=10.1007/BF02634037
Takahasi K., Sawasaki Y.
Rare spontaneously transformed human endothelial cell line provides useful research tool.
In Vitro Cell. Dev. Biol. 28:380-382(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=10653001; DOI=10.1038/labinvest.3780006
Brown J., Reading S.J., Jones S., Fitchett C.J., Howl J., Martin A., Longland C.L., Michelangeli F., Dubrova Y.E., Brown C.A.
Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83.
Lab. Invest. 80:37-45(2000)

PubMed=11669284; DOI=10.1290/1071-2690(2001)037<0505:PCOHUV>2.0.CO;2
Suda K., Rothen-Rutishauser B., Gunthert M., Wunderli-Allenspach H.
Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features.
In Vitro Cell. Dev. Biol. Anim. 37:505-514(2001)

PubMed=12197766; DOI=10.1290/1071-2690(2002)038<0185:BCCLEI>2.0.CO;2
Drexler H.G., Quentmeier H., Dirks W.G., MacLeod R.A.F.
Bladder carcinoma cell line ECV304 is not a model system for endothelial cells.
In Vitro Cell. Dev. Biol. Anim. 38:185-186(2002)

PubMed=12453433; DOI=10.1006/mvre.2002.2434
Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
Microvasc. Res. 64:384-397(2002)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=27554326; DOI=10.1038/srep31811
Xie Y.-J., Wang J.-L., Zhang Y.-Y., Liu X.-F., Wang X.-R., Liu K.-H., Huang X.-H., Wang Y.-C.
Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.
Sci. Rep. 6:31811-31811(2016)

Cross-references
Cell line collections ATCC; CRL-1998 - Discontinued
BCRJ; 0283 - Discontinued
CLS; 300452/p765_ECV-304
DSMZ; ACC-310
ECACC; 92091712
IZSLER; BS CL 137
JCRB; JCRB0744
JCRB; NIHS0220 - Discontinued
Cell line databases/resources CLDB; cl1136
CLDB; cl5185
CCRID; 3111C0002000000074
CCRID; 3142C0001000000023
Lonza; 264
Ontologies BTO; BTO:0002043
CLO; CLO_0002881
CLO; CLO_0002882
Biological sample resources BioSample; SAMN03151937
BioSample; SAMN03471274
Chemistry resources ChEMBL-Cells; CHEMBL3307513
ChEMBL-Targets; CHEMBL614035
Polymorphism and mutation databases Cosmic; 1466801
Proteomic databases PRIDE; PXD004340