Cellosaurus HCC827 (CVCL_2063)

Cell line name HCC827
Synonyms HCC-827; HCC0827
Accession CVCL_2063
Resource Identification Initiative To cite this cell line use: HCC827 (RRID:CVCL_2063)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Doubling time: 71 hours (PubMed=25984343); 28 hours (ATCC); ~50-60 hours (DSMZ).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: EGFR p.Glu746_Ala750del.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_EJ07 (HCC827 ER1); CVCL_EJ08 (HCC827 ER2); CVCL_EJ09 (HCC827 ER3); CVCL_EJ10 (HCC827 ER4); CVCL_EJ11 (HCC827 ER5); CVCL_V620 (HCC827 GR1); CVCL_V621 (HCC827 GR2); CVCL_V622 (HCC827 GR5); CVCL_V623 (HCC827 GR6); CVCL_V624 (HCC827 GR7); CVCL_V625 (HCC827 GR8); CVCL_DH92 (HCC827 PFR1); CVCL_DH93 (HCC827 PFR3); CVCL_DH94 (HCC827 PFR4); CVCL_DH95 (HCC827 PFR5); CVCL_DH96 (HCC827 PFR6); CVCL_S703 (HCC827-GR-high1); CVCL_S704 (HCC827-GR-high2); CVCL_S705 (HCC827-GR-step); CVCL_4W94 (HCC827-Luc); CVCL_EQ68 (HCC827-PR); CVCL_S748 (HCC827/R1); CVCL_S749 (HCC827/R2); CVCL_DG26 (HCC827/WR); CVCL_V407 (HCC827EPR); CVCL_V408 (HCC827ER)
Originate from same individual CVCL_S840 ! HCC827 BL
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC; Cosmic-CLP; DSMZ; KCLB; PubMed=20679594; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D13S3179
D16S53912
D18S5113
D21S1131
D3S135817
D5S81812
D7S82011,12
D8S117912
FGA22,24
Penta D14
Penta E20
TH016
TPOX8
vWA18
Web pages http://www.utsouthwestern.edu/edumedia/edufiles/about_us/admin_offices/technology_development/available_technologies/cell-lines.pdf
http://tcpaportal.org/mclp/
Publications

PubMed=10987304
Girard L., Zochbauer-Muller S., Virmani A.K., Gazdar A.F., Minna J.D.
Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering.
Cancer Res. 60:4894-4906(2000)

PubMed=11314036; DOI=10.1038/sj.onc.1204211
Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.
Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)

PubMed=20679594; DOI=10.1093/jnci/djq279
Gazdar A.F., Girard L., Lockwood W.W., Lam W.L., Minna J.D.
Lung cancer cell lines as tools for biomedical discovery and research.
J. Natl. Cancer Inst. 102:1310-1321(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23733853; DOI=10.1101/gr.152322.112
Jia P., Jin H., Meador C.B., Xia J., Ohashi K., Liu L., Pirazzoli V., Dahlman K.B., Politi K., Michor F., Zhao Z., Pao W.
Next-generation sequencing of paired tyrosine kinase inhibitor-sensitive and -resistant EGFR mutant lung cancer cell lines identifies spectrum of DNA changes associated with drug resistance.
Genome Res. 23:1434-1445(2013)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26361996; DOI=10.1016/j.jprot.2015.09.003
Grundner-Culemann K., Dybowski J.N., Klammer M., Tebbe A., Schaab C., Daub H.
Comparative proteome analysis across non-small cell lung cancer cell lines.
J. Proteomics 130:1-10(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections ATCC; CRL-2868
BCRJ; 0351
DSMZ; ACC-566
KCLB; 70827
Cell line databases/resources CCLE; HCC827_LUNG
CCRID; 3131C0001000700153
Cosmic-CLP; 1240146
GDSC; 1240146
IGRhCellID; HCC827GEO
LINCS_LDP; LCL-1608
Ontologies BTO; BTO:0004082
CLO; CLO_0003659
EFO; EFO_0003137
Biological sample resources BioSample; SAMN03472697
Gene expression databases GEO; GSM63350
GEO; GSM183436
GEO; GSM183437
GEO; GSM253425
GEO; GSM434274
GEO; GSM466319
GEO; GSM513969
GEO; GSM514323
GEO; GSM784276
GEO; GSM794382
GEO; GSM827206
GEO; GSM844553
GEO; GSM887060
GEO; GSM888130
GEO; GSM1557132
GEO; GSM1669864
Polymorphism and mutation databases Cosmic; 903634
Cosmic; 1028936
Cosmic; 1128251
Cosmic; 1146941
Cosmic; 1219071
Cosmic; 1352407
Cosmic; 1802300
Cosmic; 1995436
Cosmic; 2125263
Proteomic databases PRIDE; PXD002556