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Cellosaurus SKNO-1 (CVCL_2196)

[Text version]
Cell line name SKNO-1
Synonyms Skno-1; SKNO1
Accession CVCL_2196
Resource Identification Initiative To cite this cell line use: SKNO-1 (RRID:CVCL_2196)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: LL-100 blood cancer cell line panel.
Population: Japanese.
Characteristics: CSF2 dependent.
Doubling time: 48-72 hours (PubMed=7772516); 24 hours (PubMed=25984343); ~35-50 hours (DSMZ=ACC-690); ~38 hours (Note=Lot 06112012), 52-57 hours (Note=Lot 02032016) (JCRB=JCRB1170).
Omics: Deep exome analysis.
Omics: shRNA library screening.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene fusion; HGNC; 10471; RUNX1 + HGNC; 1535; RUNX1T1; Name(s)=RUNX1-RUNX1T1, AML1-ETO (PubMed=11753612; PubMed=15843827).
  • Mutation; HGNC; 6342; KIT; Simple; p.Asn822Lys (c.2466T>A) (N818K); ClinVar=VCV000375931; Zygosity=Homozygous (PubMed=18385756; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (PubMed=7772516; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.His368Arg (c.1103G>A); Zygosity=Homozygous (PubMed=7772516; DepMap).
HLA typing Source: DSMZCellDive=ACC-690
Class I
HLA-AA*02:06:01,31:01:02
HLA-BB*40:02:01,46:01:01
HLA-CC*01:02:01,03:04:01
Class II
HLA-DRDRA*01:01:01,01:02:02
DRB1*14:02:01,14:02:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.83
Native American0
East Asian, North90.65
East Asian, South3.5
South Asian1.23
European, North0.2
European, South2.59
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 22Y
Category Cancer cell line
STR profile Source(s): DSMZ; JCRB

Markers:
AmelogeninX
CSF1PO12,13
D2S133819
D3S135816
D5S81810,13
D7S82010,12
D8S117914,15
D13S3178,11
D16S53910,11
D18S5113,14
D19S43313,15
D21S1129,30
FGA23
Penta D9
Penta E11,12
TH017
TPOX8,9
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=7772516; DOI=10.1111/j.1365-2141.1995.tb08418.x
Matozaki S., Nakagawa T., Kawaguchi R., Aozaki R., Tsutsumi M., Murayama T., Koizumi T., Nishimura R., Isobe T., Chihara K.
Establishment of a myeloid leukaemic cell line (SKNO-1) from a patient with t(8;21) who acquired monosomy 17 during disease progression.
Br. J. Haematol. 89:805-811(1995)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10637496; DOI=10.1038/sj.leu.2401604
Drexler H.G., Fombonne S., Matsuo Y., Hu Z.-B., Hamaguchi H., Uphoff C.C.
p53 alterations in human leukemia-lymphoma cell lines: in vitro artifact or prerequisite for cell immortalization?
Leukemia 14:198-206(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11753612; DOI=10.1038/sj.leu.2402318
Xiao Z., Greaves M.F., Buffler P., Smith M.T., Segal M.R., Dicks B.M., Wiencke J.K., Wiemels J.L.
Molecular characterization of genomic AML1-ETO fusions in childhood leukemia.
Leukemia 15:1906-1913(2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=18385756; DOI=10.1038/leu.2008.61
Becker H., Pfeifer D., Afonso J.D., Nimer S.D., Veelken H., Schwabe M., Lubbert M.
Two cell lines of t(8;21) acute myeloid leukemia with activating KIT exon 17 mutation: models for the 'second hit' hypothesis.
Leukemia 22:1792-1794(2008)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x
Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218-8218(2019)

Cross-references
Cell line collections (Providers) DSMZ; ACC-690
JCRB; JCRB1170
Cell line databases/resources cancercelllines; CVCL_2196
Cell_Model_Passport; SIDM01864
DepMap; ACH-001656
DSMZCellDive; ACC-690
Biological sample resources BioSample; SAMN03473440
BioSample; SAMN10989585
Encyclopedic resources Wikidata; Q54954758
Gene expression databases ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM236805
GEO; GSM236841
Polymorphism and mutation databases Cosmic; 787478
Cosmic; 975301
Cosmic; 1149337
Cosmic; 1281353
Cosmic; 1524841
Cosmic; 2306239
Cosmic; 2690678
IARC_TP53; 16154
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number29