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Cellosaurus ATRFLOX (CVCL_2299)

[Text version]

Cell line name ATRFLOX
Synonyms ATRFLOX [Mutatect]; ATRflox/-
Accession CVCL_2299
Resource Identification Initiative To cite this cell line use: ATRFLOX (RRID:CVCL_2299)
Comments Characteristics: One copy of the ATR gene has been disrupted while lox sites have been added flanking exon 2 of the 2nd allele thus making it susceptible to Cre-mediated deletion.
Knockout cell: Method=Homologous recombination (1 of 2 alleles); HGNC; 882; ATR.
Omics: Deep RNAseq analysis.
Omics: SNP array analysis.
Derived from sampling site: Colon.
Sequence variations Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC; PubMed=25877200

Markers:
AmelogeninX
CSF1PO7,9
D3S135812,19
D5S81810,12
D7S82011,12
D8S117912,14
D13S31710,12
D16S53911,13
D18S5116,17
D21S1129,30
FGA18,23
Penta D9,13
Penta E13,14
TH018,9
TPOX8
vWA17,22

Run an STR similarity search on this cell line
Publications

PubMed=11721054; DOI=10.1126/science.1065521
Cortez D., Guntuku S., Qin J., Elledge S.J.
ATR and ATRIP: partners in checkpoint signaling.
Science 294:1713-1716(2001)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

Cross-references
Cell line collections ATCC; CRL-2780
Cell line databases/resources ColonAtlas; ATRFLOX
Ontologies CLO; CLO_0001764
EFO; EFO_0006535
Biological sample resources BioSample; SAMN03472971
Chemistry resources PharmacoDB; ATRFLOX_69_2019
Gene expression databases ArrayExpress; E-MTAB-2706
Other Wikidata; Q54751289
Entry history
Entry creation04-Apr-2012
Last entry update20-May-2021
Version number21