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Cellosaurus ATRFLOX (CVCL_2299)

[Text version]

Cell line name ATRFLOX
Synonyms ATRFLOX [Mutatect]; ATRflox/-
Accession CVCL_2299
Resource Identification Initiative To cite this cell line use: ATRFLOX (RRID:CVCL_2299)
Comments Characteristics: One copy of the ATR gene has been disrupted while lox sites have been added flanking exon 2 of the 2nd allele thus making it susceptible to Cre-mediated deletion.
Knockout cell: Method=Homologous recombination (1 of 2 alleles); HGNC; 882; ATR.
Omics: Deep RNAseq analysis.
Omics: SNP array analysis.
Sequence variations Homozygous for ACVR2A p.Lys437fs*5 (c.1310delA) (from parent cell line).
Heterozygous for BRCA2 p.Ile2675fs*6 (c.8021_8022insA) (from parent cell line).
Heterozygous for CDKN2A p.Arg24fs*20 (c.68_69insG) and p.Glu74fs*15 (c.220delG) (from parent cell line).
Heterozygous for CTNNB1 p.Ser45del (c.133_135delTCT) (from parent cell line).
Heterozygous for KRAS p.Gly13Asp (c.38G>A) (from parent cell line).
Heterozygous for PIK3CA p.His1047Arg (c.3140A>G) (from parent cell line).
Homozygous for TGFBR2 p.Lys128Serfs*35 (c.383delA) (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC; PubMed=25877200

Markers:
AmelogeninX
CSF1PO7,9
D3S135812,19
D5S81810,12
D7S82011,12
D8S117912,14
D13S31710,12
D16S53911,13
D18S5116,17
D21S1129,30
FGA18,23
Penta D9,13
Penta E13,14
TH018,9
TPOX8
vWA17,22

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Publications

PubMed=11721054; DOI=10.1126/science.1065521
Cortez D., Guntuku S., Qin J., Elledge S.J.
ATR and ATRIP: partners in checkpoint signaling.
Science 294:1713-1716(2001)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

Cross-references
Cell line collections ATCC; CRL-2780
Cell line databases/resources ColonAtlas; ATRFLOX
Ontologies CLO; CLO_0001764
EFO; EFO_0006535
Biological sample resources BioSample; SAMN03472971
Gene expression databases ArrayExpress; E-MTAB-2706
Other Wikidata; Q54751289
Entry history
Entry creation04-Apr-2012
Last entry updated07-Sep-2018
Version number16