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Cellosaurus HCT 8 (CVCL_2478)

Cell line name HCT 8
Synonyms HCT-8; HCT8
Accession CVCL_2478
Resource Identification Initiative To cite this cell line use: HCT 8 (RRID:CVCL_2478)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=25926053).
Omics: Deep RNAseq analysis.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: HCT 18; Occasionally.
Sequence variations APC p.Arg727Met (c.2180G>T), p.Lys993Asn (c.2979G>T), p.Ile1417fs*2 (c.4248delC) and p.Arg2166Ter (c.6496C>T) (PubMed=24755471).
CTNNB1 p.Val79Ile (c.235G>A) (PubMed=24755471).
KRAS p.Gly13Asp (c.38G>A) (PubMed=20570890; PubMed=24755471).
PIK3CA p.Glu545Lys (c.1633G>A) and p.Asp549Asn (c.1645G>A) (PubMed=20570890; PubMed=24755471).
SMAD2 p.Pro164fs*8 (c.490_491insA) (PubMed=24755471).
SMAD3 p.Val244Ile (c.730G>A) (PubMed=24755471).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3449 (__Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18)
Children:
CVCL_5679 (HCT-8/E11)CVCL_5945 (HCT-8/R1)CVCL_5947 (HCT-8/S10)
CVCL_5682 (HCT-8/S11)CVCL_S851 (HCT-8R)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; KCLB; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX,Y (AddexBio; KCLB; PubMed=25926053)
X (PubMed=25877200)
CSF1PO12
D13S3178,11
D16S53912,13
D18S5111,17
D21S1129,32.2
D3S135817
D5S81813
D7S82010,11.3,12 (AddexBio)
10,12 (KCLB; PubMed=25926053)
10,11.3 (PubMed=25877200)
D8S117915
FGA22
Penta D9,14
Penta E7,14
TH017,9.3
TPOX8,11
vWA18,19
Web pages http://tcpaportal.org/mclp/
Publications

PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B
Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.
Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.
Cancer 71:315-321(1993)

PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8
Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., van Roy F.M., Mareel M.M.
Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?
Cancer Genet. Cytogenet. 107:76-79(1998)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0009017/4907
ECACC; 90032006
ICLC; HTL99005
IZSLER; BS TCL 97
KCB; KCB 2011103YJ
KCLB; 10244
Cell line databases/resources CLDB; cl1577
CLDB; cl1578
CLDB; cl4949
CLDB; cl5197
CCLE; HCT8_LARGE_INTESTINE
CCRID; 3111C0001CCC000104
CCRID; 3131C0001000700018
ColonAtlas; HCT8
IGRhCellID; HCT8
LINCS_LDP; LCL-1198
Ontologies BTO; BTO:0002255
CLO; CLO_0003667
EFO; EFO_0002189
Biological sample resources BioSample; SAMN03473138
Chemistry resources ChEMBL-Cells; CHEMBL3308035
ChEMBL-Targets; CHEMBL613510
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM206504
GEO; GSM274709
GEO; GSM274710
GEO; GSM274723
GEO; GSM513910
GEO; GSM514294
GEO; GSM827344
GEO; GSM1346876
GEO; GSM1374523
GEO; GSM1374524
GEO; GSM1448162
Metabolomic databases MetaboLights; MTBLS227
Other Wikidata; Q54882008
Polymorphism and mutation databases Cosmic; 887226
Cosmic; 948853
Cosmic; 983738
Cosmic; 988873
Cosmic; 1043814
Cosmic; 1310946
Cosmic; 1479593
Cosmic; 1609484
Cosmic; 1676735
Cosmic; 2267316
Cosmic; 2301980
Cosmic; 2651867