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Cellosaurus MES-SA/Dx5 (CVCL_2598)

[Text version]
Cell line name MES-SA/Dx5
Synonyms MES-SA/Dx-5; MES-SA-Dx5; MES-SA Dx5; MESSA/Dx5; MESSA-DX5; MESSA Dx5; MESSADX5; Dx5
Accession CVCL_2598
Resource Identification Initiative To cite this cell line use: MES-SA/Dx5 (RRID:CVCL_2598)
Comments Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Doubling time: 30 hours (ECACC=95051031).
Selected for resistance to: ChEBI; CHEBI:28748; Doxorubicin (Adriablastin; Adriamycin).
Omics: Protein expression by reverse-phase protein arrays.
Omics: Proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Misspelling: MES-AS/Dx5; BCRJ=0403.
Misspelling: MES-SA/DxS; ChEMBL-Targets=CHEMBL613827; ChEMBL-Cells=CHEMBL3308373.
Derived from site: In situ; Uterus; UBERON=UBERON_0000995.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11110; ARID1A; Simple; p.Gly1610Trpfs*38 (c.4826dupC) (p.S1609fs) (c.4825_4826insC); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11110; ARID1A; Simple; p.Thr1690Asnfs*8 (c.5068dupA) (c.5067_5068insA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.His272Thrfs*4 (c.813delT) (p.Phe271fs) (c.811delT); ClinVar=VCV000428208; Zygosity=Heterozygous (from parent cell line).
Disease Uterine corpus sarcoma (NCIt: C6339)
Sarcoma of the corpus uteri (ORDO: Orphanet_213620)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1404 (MES-SA)
Children:
CVCL_4T94 (MES-SA/DxP5002)
Sex of cell Female
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): ATCC; Genomics_Center_BCF_Technion; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D1S165614,17.3
D2S44110,14
D2S133817
D3S135814,17
D5S81813
D7S8207,11 (ATCC)
11 (Genomics_Center_BCF_Technion; PubMed=25877200)
D8S117914,15
D10S124814,15
D12S39117,25
D13S31713
D16S53911,12
D18S5115
D19S43314.2,15
D21S1129,30.2
D22S104516
FGA20,25
Penta D13,15
Penta E7,10
TH016
TPOX8,11
vWA18

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/
Publications

PubMed=4028002
Harker W.G., Sikic B.I.
Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA.
Cancer Res. 45:4091-4096(1985)

PubMed=15816562
Wesolowska O., Paprocka M., Kozlak J., Motohashi N., Dus D., Michalak K.
Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening.
Anticancer Res. 25:383-389(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22889595; DOI=10.1016/j.jprot.2012.07.047
Lin S.-T., Chou H.-C., Chang S.-J., Chen Y.-W., Lyu P.-C., Wang W.-C., Chang M.D.-T., Chan H.-L.
Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells.
J. Proteomics 75:5822-5847(2012)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
Teicher B.A., Polley E.C., Kunkel M., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-1977 - Discontinued
BCRC; 60331
BCRJ; 0403
ECACC; 95051031
Cell line databases/resources CLO; CLO_0007678
CLO; CLO_0037164
CLDB; cl3449
cancercelllines; CVCL_2598
Cell_Model_Passport; SIDM01225
IGRhCellID; MESSADX5
LINCS_LDP; LCL-1510
Biological sample resources BioSample; SAMN03470985
BioSample; SAMN03471267
Chemistry resources ChEMBL-Cells; CHEMBL3308373
ChEMBL-Targets; CHEMBL613827
PubChem_Cell_line; CVCL_2598
Encyclopedic resources Wikidata; Q54905250
Gene expression databases ArrayExpress; E-MTAB-38
GEO; GSM827348
GEO; GSM1676305
GEO; GSM1701640
Polymorphism and mutation databases Progenetix; CVCL_2598
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number30