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Cellosaurus WiDr (CVCL_2760)

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Cell line name WiDr
Synonyms WiDR; WIDR; WiDr/S; WiDr-TC; WiDrTC; LED-WiDr; Led-WiDr
Accession CVCL_2760
Resource Identification Initiative To cite this cell line use: WiDr (RRID:CVCL_2760)
Comments Problematic cell line: Contaminated. Shown to be a HT-29 derivative (PubMed=3472642; PubMed=20143388). Originally thought to originate from a 78 year old female patient primary adenocarcinoma of the rectosigmoid colon.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00103.
Doubling time: 15 hours (PubMed=90012).
Microsatellite instability: Stable (MSS) (PubMed=10674020; PubMed=24042735; PubMed=25926053; PubMed=28683746).
Omics: Deep RNAseq analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Heterozygous for APC p.Glu853Ter (c.2557G>T) and p.Thr1556fs*3 (c.4666_4667insA) (from parent cell line).
Heterozygous for BRAF p.Val600Glu (c.1799T>A) (PubMed=20570890; PubMed=24042735; PubMed=28683746).
Heterozygous for PIK3CA p.Pro449Thr (c.1345C>A) (PubMed=24042735).
Homozygous for SMAD4 p.Gln311Ter (c.931C>T) (from parent cell line).
Homozygous for TP53 p.Arg273His (c.818G>A) (PubMed=24042735; PubMed=28683746).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Children:
CVCL_2N03 (WiDr/R)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; ECACC; JCRB; KCLB; PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,12
D3S135815,17
D5S81811,12
D7S82010
D8S117910
D13S31711,12 (ATCC; ECACC; KCLB; PubMed=25926053)
11 (CLS; JCRB)
D16S53911,12
D18S5113
D21S1129,30
FGA20,22
Penta D11,13
Penta E14,16
TH016,9
TPOX8,9
vWA17,19

Run an STR similarity search on this cell line
Web pages http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

PubMed=4203458
Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=90012; DOI=10.1007/BF02618407
Noguchi P., Wallace R.E., Johnson J., Earley E.M., O'Brien S., Ferrone S., Pellegrino M.A., Milstien J., Needy C., Browne W., Petricciani J.C.
Characterization of the WiDr: a human colon carcinoma cell line.
In Vitro 15:401-408(1979)

CLPUB00271
Wallace R.E., Lindh D., Durr F.E.
Studies on the development of resistance to mitoxantrone in human colon carcinoma cells in vitro.
Proc. Am. Assoc. Cancer Res. 23:767-767(1982)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3472642; DOI=10.1016/0165-4608(87)90267-6
Chen T.-R., Drabkowski D., Hay R.J., Macy M.L., Peterson W.D. Jr.
WiDr is a derivative of another colon adenocarcinoma cell line, HT-29.
Cancer Genet. Cytogenet. 27:125-134(1987)

PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=1389533; DOI=10.1016/0959-8049(92)90031-V
Lahm H., Petral-Malec D., Yilmaz-Ceyhan A., Fischer J.R., Lorenzoni M., Givel J.-C., Odartchenko N.
Growth stimulation of a human colorectal carcinoma cell line by interleukin-1 and -6 and antagonistic effects of transforming growth factor beta 1.
Eur. J. Cancer 28:1894-1899(1992)

PubMed=8464898; DOI=10.1073/pnas.90.7.2842
Browning M.J., Krausa P., Rowan A.J., Bicknell D.C., Bodmer J.G., Bodmer W.F.
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993)

PubMed=7874267; DOI=10.1007/BF02349278
Ikuta S., Itoh F., Hinoda Y., Toyota M., Makiguchi Y., Imai K., Yachi A.
Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma.
J. Gastroenterol. 29:727-732(1994)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=10956384; DOI=10.1002/1097-0215(20000915)87:6<771::AID-IJC2>3.0.CO;2-V
Backus H.H.J., Pinedo H.M., Wouters D., Padron J.M., Molders N., van Der Wilt C.L., van Groeningen C.J., Jansen G., Peters G.J.
Folate depletion increases sensitivity of solid tumor cell lines to 5-fluorouracil and antifolates.
Int. J. Cancer 87:771-778(2000)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=16854228; DOI=10.1186/1476-4598-5-29
Bandres E., Cubedo E., Agirre X., Malumbres R., Zarate R., Ramirez N., Abajo A., Navarro A., Moreno I., Monzo M., Garcia-Foncillas J.
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29-29(2006)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=23932154; DOI=10.1016/j.radonc.2013.06.032
Salendo J., Spitzner M., Kramer F., Zhang X., Jo P., Wolff H.A., Kitz J., Kaulfuss S., Beissbarth T., Dobbelstein M., Ghadimi M., Grade M., Gaedcke J.
Identification of a microRNA expression signature for chemoradiosensitivity of colorectal cancer cells, involving miRNAs-320a, -224, -132 and let7g.
Radiother. Oncol. 108:451-457(2013)

PubMed=24042735; DOI=10.1038/oncsis.2013.35
Ahmed D., Eide P.W., Eilertsen I.A., Danielsen S.A., Eknaes M., Hektoen M., Lind G.E., Lothe R.A.
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:E71-E71(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28683746; DOI=10.1186/s12943-017-0691-y
Berg K.C.G., Eide P.W., Eilertsen I.A., Johannessen B., Bruun J., Danielsen S.A., Bjornslett M., Meza-Zepeda L.A., Eknaes M., Lind G.E., Myklebost O., Skotheim R.I., Sveen A., Lothe R.A.
Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies.
Mol. Cancer 16:116-116(2017)

Cross-references
Cell line collections ATCC; CCL-218
BCRC; 60157
CLS; 300377/p484_WiDr
ECACC; 85111501
ICLC; HTL00003
JCRB; IFO50043
JCRB; JCRB0224
KCLB; 10218
NCI-DTP; WIDR
RCB; RCB1908 - Discontinued
TKG; TKG 0513
TKG; TKG 0516
Cell line databases/resources CLDB; cl4715
CLDB; cl4988
Cell_Model_Passport; SIDM00152
ColonAtlas; WIDR
FCS-free; 180-2-342-3-16-3
IGRhCellID; WIDR
LINCS_HMS; 50049
LINCS_LDP; LCL-1169
Ontologies BTO; BTO:0001483
CLO; CLO_0009600
EFO; EFO_0002389
Biological sample resources BioSample; SAMN03151797
Chemistry resources ChEMBL-Cells; CHEMBL3307674
ChEMBL-Targets; CHEMBL614647
PharmacoDB; WIDR_1660_2019
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM784020
GEO; GSM827327
GEO; GSM1006242
GEO; GSM1006243
GEO; GSM1006244
GEO; GSM1374987
GEO; GSM1374988
GEO; GSM1448091
GEO; GSM2550022
Metabolomic databases MetaboLights; MTBLS227
Other Wikidata; Q54994056
Polymorphism and mutation databases Cosmic; 738928
Cosmic; 887216
Cosmic; 897740
Cosmic; 905002
Cosmic; 1066213
Cosmic; 1122336
Cosmic; 1132695
Cosmic; 1184091
Cosmic; 1187326
Cosmic; 1218881
Cosmic; 1466826
Cosmic; 1479615
Cosmic; 1609498
Cosmic; 1676736
Cosmic; 2651871
Cosmic; 2664048
Cosmic; 2668255
IARC_TP53; 21
Entry history
Entry creation04-Apr-2012
Last entry updated19-Dec-2019
Version number28