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Cellosaurus KNS-89 (CVCL_2800)

[Text version]

Cell line name KNS-89
Synonyms KN-S89; KNS89
Accession CVCL_2800
Resource Identification Initiative To cite this cell line use: KNS-89 (RRID:CVCL_2800)
Comments Problematic cell line: Contaminated. Shown to be a U-251MG derivative (PubMed=20143388; PubMed=22570425). Originally thought to originate from a 66 year old male patient with a gliosarcoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00323.
Doubling time: 42 hours (CelloPub=CLPUB00152).
Derived from sampling site: Brain; parietal lobe.
Sequence variations Mutation; HGNC; 9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0021 (U-251MG)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
STR profile Source(s): JCRB

Markers:
AmelogeninX
CSF1PO11,12
D5S81811,12
D7S82010,12
D13S31710,11
D16S53912
TH019.3
TPOX8
vWA16,18

Run an STR similarity search on this cell line
Web pages http://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
http://iclac.org/wp-content/uploads/Cross-Contaminations_v10_distribution.xlsx
Publications

CLPUB00152
Takeshita I., Takaki T., Nakamura T., Maeyama R., Fukui M., Kitamura K.
Established cell lines derived from human gliomas.
Hum. Cell 3:255-256(1990)

PubMed=16232199; DOI=10.1111/j.1349-7006.2005.00099.x
Saigusa K., Hashimoto N., Tsuda H., Yokoi S., Maruno M., Yoshimine T., Aoyagi M., Ohno K., Imoto I., Inazawa J.
Overexpressed Skp2 within 5p amplification detected by array-based comparative genomic hybridization is associated with poor prognosis of glioblastomas.
Cancer Sci. 96:676-683(2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=22570425; DOI=10.1093/neuonc/nos072
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

Cross-references
Cell line collections JCRB; IFO50360 - Discontinued
Cell line databases/resources CGH-DB; 147-1
Biological sample resources BioSample; SAMN03151976
Other Wikidata; Q54900255
Polymorphism and mutation databases Cosmic; 687576
IARC_TP53; 21438
Entry history
Entry creation04-Apr-2012
Last entry update20-May-2021
Version number22