Cellosaurus EBC-1 (CVCL_2891)

Cell line name EBC-1
Synonyms EBC-1/original; EBC1
Accession CVCL_2891
Resource Identification Initiative To cite this cell line use: EBC-1 (RRID:CVCL_2891)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Doubling time: 42 hours (CelloPub=CLPUB00014).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Heterozygous for DDR2 p.The681Ile (c.2042C>T) (PubMed=28676216).
Disease Squamous cell lung carcinoma (NCIt: C3493)
Derived from metastatic site: Skin.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_LC83 (EBC-1 KRAS (G12D/+/+/+))CVCL_U892 (EBC-1/bone)CVCL_U893 (EBC-1/brain)
Sex of cell Male
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP; JCRB; PubMed=25877200; RCB

Markers:
AmelogeninX
CSF1PO10
D13S31712
D16S5399 (Cosmic-CLP; JCRB; PubMed=25877200; TKG)
8,9 (RCB)
D18S5112,17
D21S1129,30
D3S135815
D5S81811
D7S82010,11
D8S117914
FGA22
Penta D13
Penta E16
TH017
TPOX8
vWA16,17
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-570.html
Publications

DOI=10.2482/haigan.22.53
Hiraki S., Miyai M., Seto T., Tamura T., Watanabe Y., Ozawa S., Ikeda H., Nakata Y., Ohnoshi T., Kimura I.
Establishment of human continuous cell lines from squamous cell, adeno- and small cell carcinoma of the lung and the results of heterotransplantation.
Nihon Haigan Gakkai 22:53-58(1982)

CLPUB00014
Watanabe Y.
Experimental model of human lung cancer, Part I. Establishment and characterization of new tissue culture cell lines from human squamous cell carcinoma and adenocarcinoma of the lung.
Okayama Igakkai Zasshi 97:691-700(1985)

CLPUB00257
Watanabe Y.
Experimental model of human lung cancer, Part II Heterotransplantation of human continuous cell lines from squamous cell carcinoma and adenocarcinoma of the lung.
Okayama Igakkai Zasshi 97:701-712(1985)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=11005564; DOI=10.1038/sj.neo.7900094
Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.
Mutation and expression of the DCC gene in human lung cancer.
Neoplasia 2:300-305(2000)

PubMed=12569360; DOI=10.1038/sj.onc.1206106
Funakoshi T., Tachibana I., Hoshida Y., Kimura H., Takeda Y., Kijima T., Nishino K., Goto H., Yoneda T., Kumagai T., Osaki T., Hayashi S., Aozasa K., Kawase I.
Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer.
Oncogene 22:674-687(2003)

PubMed=14581340
Yokoi S., Yasui K., Iizasa T., Imoto I., Fujisawa T., Inazawa J.
TERC identified as a probable target within the 3q26 amplicon that is detected frequently in non-small cell lung cancers.
Clin. Cancer Res. 9:4705-4713(2003)

PubMed=15185009; DOI=10.1007/s00262-004-0533-9
Yamaji H., Iizasa T., Koh E., Suzuki M., Otsuji M., Chang H., Motohashi S., Yokoi S., Hiroshima K., Tagawa M., Nakayama T., Fujisawa T.
Correlation between interleukin 6 production and tumor proliferation in non-small cell lung cancer.
Cancer Immunol. Immunother. 53:786-792(2004)

PubMed=15746151; DOI=10.1093/hmg/ddi092
Izumi H., Inoue J., Yokoi S., Hosoda H., Shibata T., Sunamori M., Hirohashi S., Inazawa J., Imoto I.
Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers.
Hum. Mol. Genet. 14:997-1007(2005)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-I., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22313637; DOI=10.4161/cbt.19238
Takata M., Chikumi H., Miyake N., Adachi K., Kanamori Y., Yamasaki A., Igishi T., Burioka N., Nanba E., Shimizu E.
Lack of AKT activation in lung cancer cells with EGFR mutation is a novel marker of cetuximab sensitivity.
Cancer Biol. Ther. 13:369-378(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24002593; DOI=10.1038/bjc.2013.452
Wu D., Pang Y., Wilkerson M.D., Wang D., Hammerman P.S., Liu J.S.
Gene-expression data integration to squamous cell lung cancer subtypes reveals drug sensitivity.
Br. J. Cancer 109:1599-1608(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28676216; DOI=10.1016/j.lungcan.2017.05.017
Kobayashi-Watanabe N., Sato A., Watanabe T., Abe T., Nakashima C., Sueoka E., Kimura S., Sueoka-Aragane N.
Functional analysis of discoidin domain receptor 2 mutation and expression in squamous cell lung cancer.
Lung Cancer 110:35-41(2017)

Cross-references
Cell line collections JCRB; JCRB0820
RCB; RCB1965
TKG; TKG 0481
Cell line databases/resources CCLE; EBC1_LUNG
CGH-DB; 185-1
CGH-DB; 9172-4
Cosmic-CLP; 753554
GDSC; 753554
LINCS_LDP; LCL-1584
Ontologies BTO; BTO:0003001
CLO; CLO_0050039
EFO; EFO_0003115
Biological sample resources BioSample; SAMN03472045
BioSample; SAMN03472311
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM274671
GEO; GSM827595
GEO; GSM886993
GEO; GSM888062
GEO; GSM1374471
GEO; GSM1669749
Other Wikidata; Q54831756
Polymorphism and mutation databases Cosmic; 753554
Cosmic; 801583
Cosmic; 844845
Cosmic; 876170
Cosmic; 889089
Cosmic; 917995
Cosmic; 932929
Cosmic; 946069
Cosmic; 1048220
Cosmic; 1219069
Cosmic; 1239905
Cosmic; 1571731
Cosmic; 1656904
Cosmic; 1694670
Cosmic; 1802303
Cosmic; 2060527
Cosmic; 2125222
Cosmic; 2546867
Cosmic; 2645028
Cosmic; 2668327