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Cellosaurus HuH-28 (CVCL_2955)

[Text version]

Cell line name HuH-28
Synonyms HUH-28; HuH28
Accession CVCL_2955
Resource Identification Initiative To cite this cell line use: HuH-28 (RRID:CVCL_2955)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Doubling time: ~80 hours (PubMed=2852388); ~88 hours (lot 01062011), ~1 week (lot 09082006), 55-128 hours (lot 07222016) (JCRB).
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Heterozygous for PIK3CA p.Glu545Lys (c.1633G>A) (CCLE).
Homozygous for TP53 p.Glu271Lys (c.811G>A) (CCLE).
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.05
Native American0
East Asian, North84.08
East Asian, South11.01
South Asian0
European, North0
European, South1.86
Disease Intrahepatic cholangiocarcinoma (NCIt: C35417)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): JCRB; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX
CSF1PO9,12
D2S133819
D3S135815,16
D5S8189,12
D7S82010,11 (JCRB; PubMed=25877200; TKG)
10 (RCB)
D8S117911
D13S3179,12
D16S5399
D18S5115
D19S43313,14.2
D21S1130,33.2
FGA23
Penta D9,10
Penta E13,22
TH019
TPOX8
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=2852388; DOI=10.1007/BF01851205
Kusaka Y., Tokiwa T., Sato J.
Establishment and characterization of a cell line from a human cholangiocellular carcinoma.
Res. Exp. Med. (Berl.) 188:367-375(1988)

PubMed=2856443
Kusaka Y., Muraoka A., Tokiwa T., Sato J.
Establishment and characterization of a human cholangiocellular carcinoma cell line.
Hum. Cell 1:92-94(1988)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=21451941; DOI=10.1007/s00534-011-0376-7
Sato J., Kimura T., Saito T., Anazawa T., Kenjo A., Sato Y., Tsuchiya T., Gotoh M.
Gene expression analysis for predicting gemcitabine resistance in human cholangiocarcinoma.
J. Hepatobiliary Pancreat. Sci. 18:700-711(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

CLPUB00275
Zach S., Birgin E., Ruckert F.
Primary cholangiocellular carcinoma cell lines.
J. Stem Cell Res. Transplant. 2:1013-1013(2015)

PubMed=27231123; DOI=10.1158/2159-8290.CD-15-1442
Saha S.K., Gordan J.D., Kleinstiver B.P., Vu P., Najem M.S., Yeo J.-C., Shi L., Kato Y., Levin R.S., Webber J.T., Damon L.J., Egan R.K., Greninger P., McDermott U., Garnett M.J., Jenkins R.L., Rieger-Christ K.M., Sullivan T.B., Hezel A.F., Liss A.S., Mizukami Y., Goyal L., Ferrone C.R., Zhu A.X., Joung J.K., Shokat K.M., Benes C.H., Bardeesy N.
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.
Cancer Discov. 6:727-739(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31077409; DOI=10.1002/hep.30704
Sittithumcharee G., Suppramote O., Vaeteewoottacharn K., Sirisuksakun C., Jamnongsong S., Laphanuwat P., Suntiparpluacha M., Matha A., Chusorn P., Buraphat P., Kakanaporn C., Charngkaew K., Silsirivanit A., Korphaisarn K., Limsrichamrern S., Tripatara P., Pairojkul C., Wongkham S., Sampattavanich S., Okada S., Jirawatnotai S.
Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.
Hepatology 70:1614-1630(2019)

Cross-references
Cell line collections JCRB; JCRB0426
RCB; RCB1943
TKG; TKG 0438
Cell line databases/resources CCLE; HUH28_BILIARY_TRACT
DepMap; ACH-000808
Ontologies CLO; CLO_0050855
Biological sample resources BioSample; SAMN03470952
BioSample; SAMN03472037
BioSample; SAMN10987843
Chemistry resources ChEMBL-Cells; CHEMBL3308110
PharmacoDB; HuH28_638_2019
Gene expression databases ArrayExpress; E-MTAB-783
GEO; GSM887147
GEO; GSM888219
Other Wikidata; Q54896845
Polymorphism and mutation databases Cosmic; 924163
Cosmic; 1373551
Cosmic; 2674045
IARC_TP53; 28291
Entry history
Entry creation04-Apr-2012
Last entry updated19-Dec-2019
Version number19