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Cellosaurus HMCB (CVCL_3317)

[Text version]
Cell line name HMCB
Synonyms Human Melanoma Cell Bowes; Bowes melanoma cells; BOWES
Accession CVCL_3317
Resource Identification Initiative To cite this cell line use: HMCB (RRID:CVCL_3317)
Comments Group: Patented cell line.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-9607.
Population: Caucasian.
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Anecdotal: The Bowes cell line is famous for its use in the identification and purification of tissue-type plasminogen activator, tPA.
Caution: The exact relationship between HMCB (Cellosaurus=CVCL_3317) and RPMI-7262/7272/7932/7962/7972 is not clear: all are derived from patient 'Bowes' melanoma.
Sequence variations
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Trp110Ter (c.330G>A) (p.Gly125Arg, c.373G>A); ClinVar=VCV000376303; Zygosity=Homozygous (DepMap).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Unspecified (PubMed=12068308).
  • Mutation; HGNC; 11998; TP53; Simple; p.His193Arg (c.578A>G); ClinVar=VCV000184979; Zygosity=Homozygous (DepMap).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,02:01
HLA-BB*07:02,57:01
HLA-CC*06:02,07:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.21
Native American0
East Asian, North1.87
East Asian, South0
South Asian0
European, North63.92
European, South33.99
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A0LF (Bowes/sf)
Originate from same individual CVCL_T313 ! RPMI-7262
CVCL_T314 ! RPMI-7272
CVCL_T315 ! RPMI-7551
CVCL_T316 ! RPMI-7781
CVCL_T317 ! RPMI-7801
CVCL_T318 ! RPMI-7922
CVCL_2713 ! RPMI-7932
CVCL_T319 ! RPMI-7962
CVCL_T320 ! RPMI-7972
CVCL_T321 ! RPMI-8002
CVCL_T322 ! RPMI-8072
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO10
D5S81811,12
D7S82011,13
D13S3178,11
D16S53913
TH019,9.3
TPOX8
vWA17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/b/cell-lines-detail-379.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-109.html
Publications

PubMed=1970678; DOI=10.1007/BF02890069
Lombardi T., Castellucci M.
Bowes human melanoma cell line. An immunocytochemical study.
Virchows Arch. B. Cell. Pathol. Incl. Mol. Pathol. 58:181-183(1990)

Patent=US5010002
Levinson A., Simonsen C., Yelverton E.M.
Human t-PA production using vectors coding DHFR protein.
Patent number US5010002, 23-Apr-1991

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) ATCC; CRL-9607
NCBI_Iran; C524
Cell line databases/resources CLO; CLO_0003785
cancercelllines; CVCL_3317
Cell_Model_Passport; SIDM00914
DepMap; ACH-000931
IGRhCellID; HMCB
Anatomy/cell type resources BTO; BTO:0001998
Biological sample resources BioSample; SAMN10988240
Chemistry resources ChEMBL-Cells; CHEMBL3308385
ChEMBL-Targets; CHEMBL614824
PharmacoDB; HMCB_553_2019
PubChem_Cell_line; CVCL_3317
Encyclopedic resources Wikidata; Q54889913
Experimental variables resources EFO; EFO_0002195
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
GEO; GSM827473
GEO; GSM887087
GEO; GSM888157
Polymorphism and mutation databases Cosmic; 724822
IARC_TP53; 21374
LiGeA; CCLE_295
Progenetix; CVCL_3317
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number38