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Cellosaurus HuT 102 (CVCL_3526)

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Cell line name HuT 102
Synonyms HUT 102; Hut 102; HuT-102; Hut-102; HUT-102; HuT102; Hut102; HUT102; NCI-H102
Accession CVCL_3526
Resource Identification Initiative To cite this cell line use: HuT 102 (RRID:CVCL_3526)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Population: African American.
Doubling time: 38 hours (PubMed=6244013).
Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Virome analysis using RNAseq.
Anecdotal: This is the cell line that was used by Gallo's lab to isolate HTLV-1 (PubMed=26696625).
Caution: Indicated as originating from a 28 year old at sampling in PubMed=6261256, 26 year old in PubMed=6244013 and 29 year old in PubMed=8806092.
Derived from site: In situ; Lymph node; UBERON=UBERON_0000027.
Cell type: T-cell; CL=CL_0000084.
HLA typing Source: IPD-IMGT/HLA=10777
Class I
HLA-AA*66:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African84.86
Native American0.23
East Asian, North1.93
East Asian, South0
South Asian0
European, North6.73
European, South6.25
Disease Mycosis fungoides (NCIt: C3246)
Classic mycosis fungoides (ORDO: Orphanet_2584)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_8329 (HuT 102B)
Originate from same individual CVCL_B7GH ! CTCL-3
Sex of cell Male
Age at sampling 28Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; PubMed=28357668

Markers:
AmelogeninX,Y
CSF1PO8,11
D2S133819,23
D3S135815,16
D5S8188,13
D6S104311,18
D7S8208,10
D8S117913,15
D12S39116,19
D13S31711,13
D16S53912
D18S5120
D19S43314
D21S1128,29
FGA24
Penta D8,10
Penta E7,8
TH017,8
TPOX6
vWA16,19

Run an STR similarity search on this cell line
Web pages http://www.bmj.com/rapid-response/2011/10/30/cell-lines-hut102-and-hut78
https://www.atcc.org/en/support/technical-support/faqs/hut-102-atcc-tib-162-cell-growth
Publications

PubMed=6244013; DOI=10.1182/blood.V55.3.409.409
Gazdar A.F., Carney D.N., Bunn P.A. Jr., Russell E.K., Jaffe E.S., Schechter G.P., Guccion J.G.
Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
Blood 55:409-417(1980)

PubMed=6261256; DOI=10.1073/pnas.77.12.7415
Poiesz B.J., Ruscetti F.W., Gazdar A.F., Bunn P.A. Jr., Minna J.D., Gallo R.C.
Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma.
Proc. Natl. Acad. Sci. U.S.A. 77:7415-7419(1980)

PubMed=6272125; DOI=10.1038/294268a0
Poiesz B.J., Ruscetti F.W., Reitz M.S. Jr., Kalyanaraman V.S., Gallo R.C.
Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sezary T-cell leukaemia.
Nature 294:268-271(1981)

PubMed=6975346; DOI=10.1084/jem.154.5.1403
Gootenberg J.E., Ruscetti F.W., Mier J.W., Gazdar A.F., Gallo R.C.
Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.
J. Exp. Med. 154:1403-1418(1981)

PubMed=6954533; DOI=10.1073/pnas.79.7.2194
Westin E.H., Gallo R.C., Arya S.K., Eva A., Souza L.M., Baluda M.A., Aaronson S.A., Wong-Staal F.
Differential expression of the amv gene in human hematopoietic cells.
Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3001699; DOI=10.1073/pnas.82.24.8359
Kiyokawa T., Seiki M., Iwashita S., Imagawa K., Shimizu F., Yoshida M.
p27x-III and p21x-III, proteins encoded by the pX sequence of human T-cell leukemia virus type I.
Proc. Natl. Acad. Sci. U.S.A. 82:8359-8363(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=8127147
Heyman M., Grander D., Brondum-Nielsen K., Cederblad B., Liu Y., Xu B., Einhorn S.
Interferon system defects in malignant T-cells.
Leukemia 8:425-434(1994)

PubMed=7662981; DOI=10.1182/blood.V86.6.2350.bloodjournal8662350
Imada K., Takaori-Kondo A., Akagi T., Shimotohno K., Sugamura K., Hattori T., Yamabe H., Okuma M., Uchiyama T.
Tumorigenicity of human T-cell leukemia virus type I-infected cell lines in severe combined immunodeficient mice and characterization of the cells proliferating in vivo.
Blood 86:2350-2357(1995)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

PubMed=8641406; DOI=10.1111/j.1600-0609.1996.tb00721.x
Borgonovo-Brandter L., Heyman M., Rasool O., Liu Y., Grander D., Einhorn S.
p16INK4/p15INK4B gene inactivation is a frequent event in malignant T-cell lines.
Eur. J. Haematol. 56:313-318(1996)

PubMed=8806089; DOI=10.1002/jcb.240630502
Gazdar A.F., Minna J.D.
NCI series of cell lines: an historical perspective.
J. Cell. Biochem. 63 Suppl. 24:1-11(1996)

PubMed=8806090; DOI=10.1002/jcb.240630503
Bunn P.A. Jr., Foss F.M.
T-cell lymphoma cell lines (HUT102 and HUT78) established at the National Cancer Institute: history and importance to understanding the biology, clinical features, and therapy of cutaneous T-cell lymphomas (CTCL) and adult T-cell leukemia-lymphomas (ATLL).
J. Cell. Biochem. 63 Suppl. 24:12-23(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x
Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N., Liu C., Kondo E., Minowada J., Akagi T.
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=9787181; DOI=10.1182/blood.V92.9.3410
Sakai A., Thieblemont C., Wellmann A., Jaffe E.S., Raffeld M.
PTEN gene alterations in lymphoid neoplasms.
Blood 92:3410-3415(1998)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=12920029; DOI=10.1182/blood-2003-04-1043
Chung H.-K., Young H.A., Goon P.K.C., Heidecker G., Princler G.L., Shimozato O., Taylor G.P., Bangham C.R.M., Derse D.D.
Activation of interleukin-13 expression in T cells from HTLV-1-infected individuals and in chronically infected cell lines.
Blood 102:4130-4136(2003)

PubMed=15638862; DOI=10.1111/j.1365-2141.2004.05289.x
Hasegawa H., Yamada Y., Harasawa H., Tsuji T., Murata K., Sugahara K., Tsuruda K., Ikeda S., Imaizumi Y., Tomonaga M., Masuda M., Takasu N., Kamihira S.
Sensitivity of adult T-cell leukaemia lymphoma cells to tumour necrosis factor-related apoptosis-inducing ligand.
Br. J. Haematol. 128:253-265(2005)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Sandberg Y., Verhaaf B., van Gastel-Mol E.J., Wolvers-Tettero I.L.M., de Vos J., MacLeod R.A.F., Noordzij J.G., Dik W.A., van Dongen J.J.M., Langerak A.W.
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=19220422; DOI=10.1111/j.1600-0609.2009.01211.x
Kamihira S., Terada C., Sasaki D., Yanagihara K., Tsukasaki K., Hasegawa H., Yamada Y.
Aberrant p53 protein expression and function in a panel of hematopoietic cell lines with different p53 mutations.
Eur. J. Haematol. 82:301-307(2009)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25355872; DOI=10.1128/JVI.02570-14
Cao S.-B., Strong M.J., Wang X., Moss W.N., Concha M., Lin Z., O'Grady T., Baddoo M., Fewell C., Renne R., Flemington E.K.
High-throughput RNA sequencing-based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project.
J. Virol. 89:713-729(2015)

PubMed=26696625; DOI=10.1073/pnas.1521629112
Coffin J.M.
The discovery of HTLV-1, the first pathogenic human retrovirus.
Proc. Natl. Acad. Sci. U.S.A. 112:15525-15529(2015)

PubMed=28357668; DOI=10.1007/978-1-4939-6872-5_11
Raimondi V., Minuzzo S.A., Ciminale V., D'Agostino D.M.
STR profiling of HTLV-1-infected cell lines.
Methods Mol. Biol. 1582:143-154(2017)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=34794206; DOI=10.1111/cas.15204
Ikebe E., Shimosaki S., Hasegawa H., Iha H., Tsukamoto Y., Wang Y., Sasaki D., Imaizumi Y., Miyazaki Y., Yanagihara K., Hamaguchi I., Morishita K.
TAS-116 (pimitespib), a heat shock protein 90 inhibitor, shows efficacy in preclinical models of adult T-cell leukemia.
Cancer Sci. 113:684-696(2022)

Cross-references
Cell line collections (Providers) ATCC; TIB-162 - Discontinued
CCTCC; GDC0051
KCLB; 90102 - Discontinued
RCB; RCB1979 - Discontinued
TKG; TKG 0382
Cell line databases/resources CLO; CLO_0004303
CLDB; cl1776
cancercelllines; CVCL_3526
CCRID; 1101HUM-PUMC000468
CCRID; 1102HUM-NIFDC00031
Cell_Model_Passport; SIDM01734
DepMap; ACH-000612
IPD-IMGT/HLA; 10777
Lonza; 99
Biological sample resources BioSample; SAMN03471941
BioSample; SAMN10987657
Chemistry resources PharmacoDB; HuT102_646_2019
Encyclopedic resources Wikidata; Q54896907
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSE168554
GEO; GSM887153
GEO; GSM888225
Polymorphism and mutation databases Cosmic; 850197
Cosmic; 933552
Cosmic; 2361371
IARC_TP53; 27004
LiGeA; CCLE_120
Progenetix; CVCL_3526
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number35