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Cellosaurus LNZTA3WT4 (CVCL_3540)

[Text version]
Cell line name LNZTA3WT4
Synonyms LNZTA3p53WT4; WT4
Accession CVCL_3540
Resource Identification Initiative To cite this cell line use: LNZTA3WT4 (RRID:CVCL_3540)
Comments Group: Patented cell line.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11543.
Population: Caucasian.
Characteristics: The expression of the luciferase reporter gene as well as the TP53 gene are under the control of a tetO operator.
Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Microsatellite instability: Stable (MSS) (Sanger).
Transfected with: HGNC; 11998; TP53.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Brain, right parieto-occipital lobe.
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.42
Native American1.36
East Asian, North1.52
East Asian, South0
South Asian1.65
European, North44.35
European, South49.7
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0394 (LN-308)
Sex of cell Male
Age at sampling 60Y
Category Cancer cell line
STR profile Source(s): ATCC; Cosmic-CLP

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81812
D7S82010,12
D13S31711
D16S5399,12
TH019.3
TPOX8,9
vWA15,17

Run an STR similarity search on this cell line
Publications

Patent=US5807731
Van Meir E.G., Huang H.-J.S., Cavenee W.K.
Glioblastoma cell lines that produce angiogenesis inhibiting factor.
Patent number US5807731, 15-Sep-1998

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line databases/resources CLO; CLO_0007371
cancercelllines; CVCL_3540
Cell_Model_Passport; SIDM01220
Cosmic-CLP; 1240170
DepMap; ACH-002269
LINCS_HMS; 50028
LINCS_LDP; LCL-1345
Biological sample resources BioSample; SAMN03471340
CRISP screens repositories BioGRID_ORCS_Cell_line; 931
Chemistry resources GDSC; 1240170
PharmacoDB; LNZTA3WT4_853_2019
Encyclopedic resources Wikidata; Q54902866
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM1670052
Polymorphism and mutation databases Cosmic; 1995487
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number28