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Cellosaurus NS0 (CVCL_3940)

[Text version]

Cell line name NS0
Synonyms NSO; NS/0; NS/O
Accession CVCL_3940
Resource Identification Initiative To cite this cell line use: NS0 (RRID:CVCL_3940)
Comments Group: Hybridoma fusion partner cell line.
Characteristics: Commonly employed for monoclonal antibody expression.
Characteristics: Lacks endogenous glutamine synthetase (GS) activity.
Selected for resistance to: ChEBI; CHEBI:63486; 8-azaguanine.
Disease Mouse plasma cell myeloma (NCIt: C125417)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_2155 (P3/NS1/1-Ag4.1)
Children:
CVCL_6494 (022HU-NSO )CVCL_A0HG (10E4 )CVCL_LC04 (11BD-2E11-2 )
CVCL_A0HH (16A3 )CVCL_DN54 (17G1 )CVCL_A0HV (1A-3187 )
CVCL_A0HW (1A-4246 )CVCL_LC05 (1A245.6 )CVCL_A0HX (1B-3246 )
CVCL_A0HY (1B-3440 )CVCL_A0HZ (1B-4863 )CVCL_A0IA (1B-4971 )
CVCL_A0HI (1B5 [Mouse hybridoma against human RPSA] )CVCL_CZ91 (1CB4 )CVCL_A0HJ (1D2 [Mouse hybridoma against human RPSA] )
CVCL_B6TF (1D4 [Mouse hybridoma against influenza D virus HE] )CVCL_B6TP (1E1 [Mouse hybridoma against influenza D virus HE] )CVCL_A0CA (1G10 [Mouse hybridoma against HIV-1 rev] )
CVCL_A0CB (1G7 [Mouse hybridoma against HIV-1 rev] )CVCL_J659 (2-179-E11 )CVCL_9149 (20C2 )
CVCL_A0HK (21H1 )CVCL_B6TG (2E11 [Mouse hybridoma against influenza D virus HE] )CVCL_B6TH (2E12 [Mouse hybridoma against influenza D virus HE] )
CVCL_B6TI (2E3 )CVCL_B6TJ (2E9 [Mouse hybridoma against influenza D virus HE] )CVCL_A0HL (2H9 [Mouse hybridoma against human RPSA] )
CVCL_J666 (357-101-4 )CVCL_J667 (357-167-24 )CVCL_J668 (359-238-8 )
CVCL_J669 (359-81-11 )CVCL_A0HM (3E6 [Mouse hybridoma against human RPSA] )CVCL_B6TK (3G3 [Mouse hybridoma against influenza D virus HE] )
CVCL_A0HN (3G5 [Mouse hybridoma against human RPSA] )CVCL_2H34 (3NB12.13 )CVCL_G693 (41-2 )
CVCL_A0HP (4C11 [Mouse hybridoma against human RPSA] )CVCL_A0HQ (4F6 [Mouse hybridoma against human RPSA] )CVCL_B6TL (4G4 [Mouse hybridoma against influenza D virus HE] )
CVCL_G694 (50-6 )CVCL_J061 (53B3 )CVCL_J062 (54B3 )
CVCL_B6TM (5D4 )CVCL_B6TN (5D6 )CVCL_XI16 (6/22-10-30-13 )
CVCL_B6ZE (6F3H )CVCL_A0HR (7A5 )CVCL_G696 (7E12H12 )
CVCL_A0HS (8E4 [Mouse hybridoma against human RPSA] )CVCL_A0CC (8E7 [Mouse hybridoma against HIV-1 rev] )CVCL_A0HU (9D8 [Mouse hybridoma against human RPSA] )
CVCL_A0CD (9G2 [Mouse hybridoma against HIV-1 rev] )CVCL_L815 (AFRC 17D1 )CVCL_K191 (AFRC MAC 156 )
CVCL_K192 (AFRC MAC 157 )CVCL_K193 (AFRC MAC 158 )CVCL_K194 (AFRC MAC 159 )
CVCL_K195 (AFRC MAC 160 )CVCL_K202 (AFRC MAC 257 )CVCL_L827 (AFRC MAC 260 )
CVCL_LB97 (AR40A603.13 )CVCL_LH69 (AR47A6.4.2 )CVCL_LH70 (AR52A301.5 )
CVCL_LB98 (AR53A10.11 )CVCL_LB99 (AR58A258.8 )CVCL_LC00 (AR58A314.1 )
CVCL_1954 (BA-D5 )CVCL_1955 (BA-F8 )CVCL_G695 (BA-G5 )
CVCL_1960 (BF-32 )CVCL_1961 (BF-34 )CVCL_1962 (BF-45 )
CVCL_KA95 (BF-B6 )CVCL_1963 (BF-F3 )CVCL_1964 (BF-G6 )
CVCL_G162 (BMAC-1 )CVCL_G163 (BMAC-3 )CVCL_CW56 (CB-16 )
CVCL_A6QQ (CK-2A7 )CVCL_A6QK (CK-JIL )CVCL_VK51 (CT-1 [Mouse hybridoma against human TNF] )
CVCL_G692 (DII 33.1 )CVCL_B6NX (EA2.31 )CVCL_B6NY (EA5.12 )
CVCL_B6SD (FC-2.15 )CVCL_DF71 (HB 34-1 )CVCL_A5YP (HET-37 )
CVCL_A9EN (HRP 16-1 )CVCL_A3CM (KT112 )CVCL_A3CL (KT15 )
CVCL_A3CJ (KT50 )CVCL_A3CK (KT65 )CVCL_L704 (LDS 73/LW1 )
CVCL_UW14 (Luc63.2.22 )CVCL_UW15 (Luc90 )CVCL_J222 (MH01 )
CVCL_K161 (NI 1 alpha 364/3B3-14 )CVCL_K162 (NI 1 alpha 364/8C4-8 )CVCL_J845 (NI 3 416/6C6-6 )
CVCL_J846 (NI 3 416/7B5-5 )CVCL_J847 (NI 4 426/1A6-10 )CVCL_J848 (NI 4 426/7A3-3 )
CVCL_J849 (NI 4 426/8B1-7 )CVCL_J850 (NI G 386/3B6-12 )CVCL_J851 (NI G 386/3D1-16 )
CVCL_J852 (NI GM 465/7A6-37 )CVCL_J853 (NORIg 7.16.2 )CVCL_G345 (NS0-SFM )
CVCL_G346 (NS0-Turbodoma )CVCL_DD16 (NS0bcl-2 )CVCL_U023 (OX-23 )
CVCL_U024 (OX-24 )CVCL_J181 (OX-26 )CVCL_J183 (OX-29 )
CVCL_J185 (OX-30 )CVCL_J186 (OX-33 )CVCL_J187 (OX-34 )
CVCL_J188 (OX-35 clone 5 )CVCL_J189 (OX-36 clone 3 )CVCL_J191 (OX-39 )
CVCL_J193 (OX-41 )CVCL_J194 (OX-42 )CVCL_J195 (OX-43 )
CVCL_J196 (OX-44 )CVCL_J197 (OX-45 )CVCL_U025 (OX-46 )
CVCL_J198 (OX-47 )CVCL_X512 (OX-48 )CVCL_J199 (OX-49 )
CVCL_J200 (OX-50 )CVCL_J201 (OX-52 )CVCL_J202 (OX-53 )
CVCL_J203 (OX-54 )CVCL_J204 (OX-55 )CVCL_J209 (OX-60 )
CVCL_J210 (OX-61 )CVCL_J211 (OX-62 )CVCL_J212 (OX-68 )
CVCL_2183 (RV-C2 )CVCL_2184 (SC-71 )CVCL_2185 (SC-75 )
CVCL_2216 (TI-1 [Mouse hybridoma] )CVCL_B7NK (TI-3 )CVCL_2217 (TI-4 )
CVCL_KA81 (VPM 1 )CVCL_KA82 (VPM 14 )CVCL_KA83 (VPM 15 )
CVCL_KA84 (VPM 16 )CVCL_KA85 (VPM 17 )CVCL_G164 (VPM 18 )
CVCL_G165 (VPM 19 )CVCL_KA89 (VPM 29 )CVCL_KA86 (VPM 3 )
CVCL_KR64 (VPM 30 )CVCL_G166 (VPM 36 )CVCL_G167 (VPM 37 )
CVCL_G168 (VPM 38 )CVCL_KA87 (VPM 39 )CVCL_KA88 (VPM 4 )
CVCL_G169 (VPM 40 )CVCL_G170 (VPM 41 )CVCL_G171 (VPM 43 )
CVCL_G172 (VPM 44 )CVCL_G173 (VPM 45 )CVCL_G174 (VPM 46 )
CVCL_G175 (VPM 47 )CVCL_G176 (VPM 48 )CVCL_G177 (VPM 5 )
CVCL_G178 (VPM 54 )CVCL_G179 (VPM 6 )CVCL_G180 (VPM 8 )
Sex of cell Female
Category Cancer cell line
Web pages https://en.wikipedia.org/wiki/NS0_cell
Publications

PubMed=19003032; DOI=10.1007/s10616-005-3563-z
Zhang J., Robinson D.
Development of animal-free, protein-free and chemically-defined media for NS0 cell culture.
Cytotechnology 48:59-74(2005)

PubMed=18617141; DOI=10.1016/j.jprot.2008.02.003
de la Luz-Hernandez K.R., Rojas-del Calvo L., Rabasa-Legon Y., Lage-Castellanos A., Castillo-Vitlloch A., Diaz J., Gaskell S.
Metabolic and proteomic study of NS0 myeloma cell line following the adaptation to protein-free medium.
J. Proteomics 71:133-147(2008)

Cross-references
Cell line collections ECACC; 85110503
IZSLER; BS TCL 48
NCBI_Iran; C142
RCB; RCB0213
TKG; TKG 0594
Cell line databases/resources CLO; CLO_0008203
CLO; CLO_0008206
CLO; CLO_0050179
CLDB; cl3737
CLDB; cl3738
CLDB; cl3739
FCS-free; 110-17-508-1-16-3
Lonza; 757
Anatomy/cell type resources BTO; BTO:0004968
Chemistry resources ChEMBL-Cells; CHEMBL3307752
ChEMBL-Targets; CHEMBL615003
Encyclopedic resources Wikidata; Q20164415
Entry history
Entry creation04-Apr-2012
Last entry update02-Jul-2020
Version number14