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Cellosaurus U-CH1 (CVCL_4988)

[Text version]

Cell line name U-CH1
Synonyms UCH-1; UCH1
Accession CVCL_4988
Resource Identification Initiative To cite this cell line use: U-CH1 (RRID:CVCL_4988)
Comments Part of: ENCODE project common cell types; tier 3.
Doubling time: ~1 week, but can be significantly longer after recovery from liquid nitrogen freezing (DSMZ); 2 days (PubMed=26183925); 9 days (Chordoma_Foundation).
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Omics: Transcriptome analysis.
Sequence variations TP53 p.Pro72Arg (c.215C>G) (ClinVar=VCV000012351) (PubMed=28515451).
HLA typing Source: Chordoma_Foundation
Class I
HLA-AA*2
Disease Sacral chordoma (NCIt: C129927)
Chordoma (ORDO: Orphanet_178)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_4990 (U-CH1-N)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): ATCC; DSMZ; PubMed=21253487; PubMed=26183925; PubMed=27102572; PubMed=28835717

Markers:
AmelogeninX,Y
CSF1PO10,11
D1S165616,16.3
D2S44112,14
D2S133817,18
D3S135815
D5S81811,12
D7S8209,12
D8S117910,15
D10S124815
D12S39117,22
D13S31711,13
D16S53912,13
D18S5115
D19S43314
D21S1128,29
D22S104516
FGA20,21
Penta D11
Penta E7,10
TH017
TPOX8,11
vWA17

Run an STR similarity search on this cell line
Web pages https://www.chordomafoundation.org/research/disease-models/u-ch1/
http://genome.ucsc.edu/ENCODE/protocols/cell/human/UCH1_Crawford_protocol.pdf
Publications

PubMed=11579460; DOI=10.1002/gcc.1184
Scheil S., Bruderlein S., Liehr T., Starke H., Herms J., Schulte M., Moller P.
Genome-wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U-CH1.
Genes Chromosomes Cancer 32:203-211(2001)

PubMed=20461036; DOI=10.1097/BRS.0b013e3181c2a8b0
Yang C., Hornicek F.J., Wood K.B., Schwab J.H., Choy E., Iafrate A.J., Rosenberg A.E., Nielsen G.P., Xavier R.J., Mankin H.J., Duan Z.
Characterization and analysis of human chordoma cell lines.
Spine 35:1257-1264(2010)

PubMed=21253487; DOI=10.1155/2010/630129
Bruderlein S., Sommer J.B., Meltzer P.S., Li S., Osada T., Ng D., Moller P., Alcorta D.A., Kelley M.J.
Molecular characterization of putative chordoma cell lines.
Sarcoma 2010:630129-630129(2010)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=26183925; DOI=10.1158/0008-5472.CAN-14-3270
von Witzleben A., Goerttler L.T., Marienfeld R., Barth H., Lechel A., Mellert K., Bohm M., Kornmann M., Mayer-Steinacker R., von Baer A., Schultheiss M., Flanagan A.M., Moller P., Bruderlein S., Barth T.F.E.
Preclinical characterization of novel chordoma cell systems and their targeting by pharmocological inhibitors of the CDK4/6 cell-cycle pathway.
Cancer Res. 75:3823-3831(2015)

PubMed=27102572; DOI=10.1002/path.4729
Scheipl S., Barnard M., Cottone L., Jorgensen M., Drewry D.H., Zuercher W.J., Turlais F., Ye H., Leite A.P., Smith J.A., Leithner A., Moller P., Bruderlein S., Guppy N., Amary F., Tirabosco R., Strauss S.J., Pillay N., Flanagan A.M.
EGFR inhibitors identified as a potential treatment for chordoma in a focused compound screen.
J. Pathol. 239:320-334(2016)

PubMed=28515451; DOI=10.1038/s41598-017-02174-5
Jager D., Barth T.F.E., Bruderlein S., Scheuerle A., Rinner B., von Witzleben A., Lechel A., Meyer P., Mayer-Steinacker R., Baer A.V., Schultheiss M., Wirtz C.R., Moller P., Mellert K.
HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas.
Sci. Rep. 7:2032-2032(2017)

PubMed=28606919; DOI=10.1158/1078-0432.CCR-17-0177
Chen H., Garbutt C.C., Spentzos D., Choy E., Hornicek F.J., Duan Z.
Expression and therapeutic potential of SOX9 in chordoma.
Clin. Cancer Res. 23:5176-5186(2017)

PubMed=28835717; DOI=10.1038/s41598-017-10044-3
Bosotti R., Magnaghi P., Di Bella S., Cozzi L., Cusi C., Bozzi F., Beltrami N., Carapezza G., Ballinari D., Amboldi N., Lupi R., Somaschini A., Raddrizzani L., Salom B., Galvani A., Stacchiotti S., Tamborini E., Isacchi A.
Establishment and genomic characterization of the new chordoma cell line Chor-IN-1.
Sci. Rep. 7:9226-9226(2017)

Cross-references
Cell line collections ATCC; CRL-3217
DSMZ; ACC-748
Cell line databases/resources DepMap; ACH-002335
Ontologies EFO; EFO_0004389
Biological sample resources ENCODE; ENCBS267AAA
Chemistry resources GDSC; 1503374
PharmacoDB; UCH1_1626_2019
Gene expression databases GEO; GSM1673845
GEO; GSM1673846
Other Wikidata; Q54973610
Polymorphism and mutation databases Cosmic; 2663857
Entry history
Entry creation04-Apr-2012
Last entry update02-Jul-2020
Version number25