Cellosaurus U-CH2 (CVCL_4989)

Cell line name U-CH2
Synonyms UCH2
Accession CVCL_4989
Resource Identification Initiative To cite this cell line use: U-CH2 (RRID:CVCL_4989)
Comments Doubling time: 4 days (PubMed=26183925); ~5-7 days (DSMZ).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Disease Sacral chordoma (NCIt: C129927)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC; Cosmic-CLP; DSMZ; PubMed=21253487; PubMed=26183925; PubMed=27102572; PubMed=28835717

Markers:
AmelogeninX
CSF1PO11,12
D10S124813,16
D12S39118,19
D13S31711
D16S53912
D18S5112,18
D19S43315.2,17
D1S165614.3,15
D21S1129,30
D22S104515
D2S133824
D2S44110,11.3
D3S135817
D5S81810,11
D7S8208,12
D8S117913
FGA21,22.2
Penta D12,13
Penta E12,15
TH019.3 (ATCC; DSMZ; PubMed=26183925; PubMed=27102572)
6 (Cosmic-CLP)
6,9.3 (PubMed=21253487)
TPOX8
vWA17
Web pages https://www.chordomafoundation.org/research/disease-models/u-ch2/
http://genome.ucsc.edu/ENCODE/protocols/cell/human/UCH1_Crawford_protocol.pdf
Publications

PubMed=21253487; DOI=10.1155/2010/630129
Bruderlein S., Sommer J.B., Meltzer P.S., Li S., Osada T., Ng D., Moller P., Alcorta D.A., Kelley M.J.
Molecular characterization of putative chordoma cell lines.
Sarcoma 2010:630129-630129(2010)

PubMed=26183925; DOI=10.1158/0008-5472.CAN-14-3270
von Witzleben A., Goerttler L.T., Marienfeld R., Barth H., Lechel A., Mellert K., Bohm M., Kornmann M., Mayer-Steinacker R., von Baer A., Schultheiss M., Flanagan A.M., Moller P., Bruderlein S., Barth T.F.E.
Preclinical characterization of novel chordoma cell systems and their targeting by pharmocological inhibitors of the CDK4/6 cell-cycle pathway.
Cancer Res. 75:3823-3831(2015)

PubMed=27102572; DOI=10.1002/path.4729
Scheipl S., Barnard M., Cottone L., Jorgensen M., Drewry D.H., Zuercher W.J., Turlais F., Ye H., Leite A.P., Smith J.A., Leithner A., Moller P., Bruderlein S., Guppy N., Amary F., Tirabosco R., Strauss S.J., Pillay N., Flanagan A.M.
EGFR inhibitors identified as a potential treatment for chordoma in a focused compound screen.
J. Pathol. 239:320-334(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28835717; DOI=10.1038/s41598-017-10044-3
Bosotti R., Magnaghi P., Di Bella S., Cozzi L., Cusi C., Bozzi F., Beltrami N., Carapezza G., Ballinari D., Amboldi N., Lupi R., Somaschini A., Raddrizzani L., Salom B., Galvani A., Stacchiotti S., Tamborini E., Isacchi A.
Establishment and genomic characterization of the new chordoma cell line Chor-IN-1.
Sci. Rep. 7:9226-9226(2017)

Cross-references
Cell line collections ATCC; CRL-3218
DSMZ; ACC-749
Cell line databases/resources Cosmic-CLP; 1503373
Gene expression databases GEO; GSM1670565