ID   TMNK-1
AC   CVCL_4W79
SY   TMNK1
DR   JCRB; JCRB1564
DR   Wikidata; Q54972700
RX   Patent=WO2008066199A1;
RX   PubMed=15167590;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8017.
CC   Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype.
CC   Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-NeoR-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
CC   Transfected with: HGNC; 11730; TERT.
CC   Transfected with: UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Endothelial cell of hepatic sinusoid; CL=CL_1000398.
ST   Source(s): JCRB
ST   Amelogenin: X
ST   CSF1PO: 10
ST   D13S317: 11
ST   D16S539: 10,11
ST   D5S818: 11
ST   D7S820: 11,13
ST   TH01: 6,7
ST   TPOX: 9
ST   vWA: 15,16
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Age unspecified
CA   Transformed cell line
DT   Created: 14-12-15; Last updated: 05-10-23; Version: 13
//
RX   Patent=WO2008066199A1;
RA   Kobayashi N., Rivas-Carrillo J.D., Tanaka N.;
RT   "Method of inducing differentiation of embryonic stem cells into
RT   insulin-secreting cells, insulin-secreting cells induced by the method
RT   and use of the same.";
RL   Patent number WO2008066199A1, 05-Jun-2008.
//
RX   PubMed=15167590; DOI=10.1097/01.TP.0000124286.82961.7E;
RA   Matsumura T., Takesue M., Westerman K.A., Okitsu T., Sakaguchi M.,
RA   Fukazawa T., Totsugawa T., Noguchi H., Yamamoto S., Stolz D.B.,
RA   Tanaka N., Leboulch P., Kobayashi N.;
RT   "Establishment of an immortalized human-liver endothelial cell line
RT   with SV40T and hTERT.";
RL   Transplantation 77:1357-1365(2004).
//