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Cellosaurus MEF (DR4) (CVCL_5277)

Cell line name MEF (DR4)
Synonyms DR4 MEF; DR-4 MEF
Accession CVCL_5277
Resource Identification Initiative To cite this cell line use: MEF (DR4) (RRID:CVCL_5277)
Comments Characteristics: Expresses transgenes that make this cell line resistant to four different drugs: geneticin (G418), puromycin, hygromycin and 6-tioguanine.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: DR4.
Hierarchy Children:
CVCL_VK72 (MEF (DR4) IRR)CVCL_Y468 (MEF (DR4) MITC)
Sex of cell Mixed sex
Age at sampling 14FD
Category Finite cell line
Publications

PubMed=9278500; DOI=10.1093/nar/25.18.3745
Tucker K.L., Wang Y.-K., Dausman J., Jaenisch R.
A transgenic mouse strain expressing four drug-selectable marker genes.
Nucleic Acids Res. 25:3745-3746(1997)

Cross-references
Cell line collections (Providers) ATCC; SCRC-1045
Cell line databases/resources CLO; CLO_0002814
CCRID; 3101MOUSCSP108
Encyclopedic resources Wikidata; Q54905024
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number12