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Cellosaurus MC3T3-E1 Subclone 4 (CVCL_5440)

[Text version]
Cell line name MC3T3-E1 Subclone 4
Synonyms MC3T3, E1 subgroup-4 clone; MC3T3-E1(C4); MC-4
Accession CVCL_5440
Resource Identification Initiative To cite this cell line use: MC3T3-E1 Subclone 4 (RRID:CVCL_5440)
Comments Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC=CRL-2593).
Doubling time: 51.6 hours (CelloPub=CLPUB00625); ~38 hours (ATCC=CRL-2593).
Miscellaneous: STR profile from personal communication of Almeida J.L.; PubMed=23430347 has a different value for STR 6-4 (17) than that submitted (18) due to a change in the marker motif.
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0409 (MC3T3-E1)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
STR profile Source(s): Direct_author_submission; PubMed=23430347

Markers:
Mouse STR 1-116,17
Mouse STR 1-219
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-220.3
Mouse STR 5-517
Mouse STR 6-418
Mouse STR 6-717
Mouse STR 7-126.2
Mouse STR 8-116
Mouse STR 11-216
Mouse STR 12-117
Mouse STR 13-116
Mouse STR 15-322.3
Mouse STR 17-216
Mouse STR 18-315
Mouse STR 19-213
Mouse STR X-128

Run an STR similarity search on this cell line
Publications

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H., Franceschi R.T.
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)

CLPUB00625
Lau P., Hellweg C.E., Kirchner S., Arenz A., Baumstark-Khan C., Horneck G.
Induction of DNA-strand breaks after X-irradiation in murine bone cells of various differentiation capacities.
(In) Proceedings of 35th COSPAR Scientific Assembly; pp.1960-1960; Paris (2004)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

Cross-references
Cell line collections (Providers) ATCC; CRL-2593
IZSLER; BS CL 181
Cell line databases/resources CLO; CLO_0007586
TOKU-E; 2372
Anatomy/cell type resources BTO; BTO:0004473
Encyclopedic resources Wikidata; Q54904205
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number18