| Cell line name |
HT29-18-N2 |
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| Synonyms |
HT29-18N2; HT-29-18 N2; N2 |
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| Accession |
CVCL_5942 |
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| Resource Identification Initiative |
To cite this cell line use: HT29-18-N2 (RRID:CVCL_5942) |
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| Comments |
Population: Caucasian.
Characteristics: Mucus-secreting clone of parent cell line. When grown in the absence of glucose they form homogeneous epithelial monolayers of columnar cells with typical goblet cell morphology (PubMed=3360261).
Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
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| Sequence variations |
- Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
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| Disease |
Colon adenocarcinoma (NCIt: C4349) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_5940 (HT29-18) |
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| Sex of cell |
Female |
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| Age at sampling |
44Y |
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| Category |
Cancer cell line |
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| Publications | PubMed=3611191; DOI=10.1083/jcb.105.1.345
Huet C., Sahuquillo-Merino C., Coudrier E., Louvard D.
Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation.
J. Cell Biol. 105:345-357(1987) PubMed=3360261; DOI=10.1016/0016-5085(88)90678-6
Phillips T.E., Huet C., Bilbo P.R., Podolsky D.K., Louvard D., Neutra M.R.
Human intestinal goblet cells in monolayer culture: characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line.
Gastroenterology 94:1390-1403(1988) PubMed=2154122; DOI=10.1152/ajpgi.1990.258.1.G96
Lencer W.I., Reinhart F.D., Neutra M.R.
Interaction of cholera toxin with cloned human goblet cells in monolayer culture.
Am. J. Physiol. 258:G96-102(1990) PubMed=8589882; DOI=10.1007/BF02634251
Phillips T.E., Ramos R., Duncan S.L.
HT29-18N2 differentiation in a protein-free medium.
In Vitro Cell. Dev. Biol. Anim. 31:421-423(1995) PubMed=12388101; DOI=10.1152/ajpcell.00010.2002
Steinbrecher K.A., Rudolph J.A., Luo G.-J., Cohen M.B.
Coordinate upregulation of guanylin and uroguanylin expression by hypertonicity in HT29-18-N2 cells.
Am. J. Physiol. Cell Physiol. 283:C1729-C1737(2002) |
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| Cross-references |
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| Cell line databases/resources |
cancercelllines; CVCL_5942
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| Encyclopedic resources |
Wikidata; Q54896589
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| Entry history |
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| Entry creation | 04-Apr-2012 |
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| Last entry update | 05-Oct-2023 |
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| Version number | 23 |
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