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Cellosaurus KM12-L4 (CVCL_5946)

[Text version]
Cell line name KM12-L4
Synonyms KM12L4; Km12L4; KM12 L4; Km 12 L4
Accession CVCL_5946
Resource Identification Initiative To cite this cell line use: KM12-L4 (RRID:CVCL_5946)
Comments Population: Caucasian.
Characteristics: Highly liver metastatic. Established after four passages into the cecum of nude mice (PubMed=3349467).
Microsatellite instability: Instable (MSI) (PubMed=25926053).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene fusion; HGNC; 8031; NTRK1 + HGNC; 12012; TPM3; Name(s)=TPM3-NTRK1 (from parent cell line).
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Asn1819fs*7 (c.5454_5455insA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Gly129Ter (c.385G>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1331 (KM12)
Children:
CVCL_W220 (KM12-L4A)CVCL_5920 (KM12-OxR)CVCL_AU12 (KM12L4/OXR)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): PubMed=25926053

Markers:
AmelogeninX
CSF1PO10,12
D5S81811
D7S8208,9
D13S31713,15
D16S53911,12
D21S1127,34
TH0110
TPOX11,12
vWA16,17

Run an STR similarity search on this cell line
Publications

PubMed=2846163
Morikawa K., Walker S.M., Nakajima M., Pathak S., Jessup J.M., Fidler I.J.
Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice.
Cancer Res. 48:6863-6871(1988)

PubMed=3349467
Morikawa K., Walker S.M., Jessup J.M., Fidler I.J.
In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice.
Cancer Res. 48:1943-1948(1988)

PubMed=8909255
Goetz D.J., Ding H., Atkinson W.J., Vachino G., Camphausen R.T., Cumming D.A., Luscinskas F.W.
A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism.
Am. J. Pathol. 149:1661-1673(1996)

PubMed=10091941; DOI=10.1023/A:1006537609469
Nakayama Y., Okazaki K., Shibao K., Sako T., Hirata K., Nagata N., Kuwano M., Itoh H.
Alterative expression of the collagenase and adhesion molecules in the highly metastatic clones of human colonic cancer cell lines.
Clin. Exp. Metastasis 16:461-469(1998)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

Cross-references
Cell line collections (Providers) KCLB; 80014
Cell line databases/resources cancercelllines; CVCL_5946
Encyclopedic resources Wikidata; Q54900057
Gene expression databases GEO; GSM1448187
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number21