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Cellosaurus MDA-MB-435s-mKate2 (CVCL_5T70)

[Text version]

Cell line name MDA-MB-435s-mKate2
Accession CVCL_5T70
Resource Identification Initiative To cite this cell line use: MDA-MB-435s-mKate2 (RRID:CVCL_5T70)
Comments Problematic cell line: Contaminated. Grand-parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Transfected with: mKate2; a bright red fluorescent protein derived from eqFP578.
Sequence variations Heterozygous for BRAF p.Val600Glu (c.1799T>A) (ClinVar=VCV000013961) (from parent cell line).
Heterozygous for CDKN2A c.150+2T>C (IVS1+2T>C); splice donor mutation and c.455insCdel26 (from parent cell line).
Hemizygous for EP300 p.Leu827Pro (c.3679T>C) and p.Glu1013Gly (4237A>G) (from parent cell line).
Heterozygous for TP53 p.Gly266Glu (c.797G>A) (ClinVar=VCV000161516) (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Subcutaneous; right buttock.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0622 (MDA-MB-435S)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line

PubMed=25902968; DOI=10.1007/s11307-015-0853-5
Vuletic I., Liu J.-H., Wu H.-L., Ding Y.-C., Lei Y., Li C.-H., Zhu D.-H., Ren Q.-S., Sun H.-F., Li J.
Establishment of an mKate2-expressing cell line for non-invasive real-time breast cancer in vivo imaging.
Mol. Imaging Biol. 17:811-818(2015)

Other Wikidata; Q54904638
Entry history
Entry creation23-Feb-2016
Last entry update02-Jul-2020
Version number10