Cellosaurus MDA-kb2 (CVCL_6421)

Cell line name MDA-kb2
Synonyms MDA-Kb2
Accession CVCL_6421
Resource Identification Initiative To cite this cell line use: MDA-kb2 (RRID:CVCL_6421)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Biotechnology: Can be used in an vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.
Characteristics: Expresses luciferase under the control of a MMTV promoter that contains glucocorticoid receptor and androgen receptor response elements.
Sequence variation: AR p.Gln868His (from parent cell line).
Sequence variation: KRAS p.Gly13Asp (from parent cell line).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Disease Breast adenocarcinoma (NCIt: C5214)
Derived from metastatic site: Pericardial effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0418 (MDA-MB-453)
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO10,12
D13S31712
D16S5399
D5S81811
D7S82010
TH016
TPOX10
vWA17,18
Publications

PubMed=11861974; DOI=10.1093/toxsci/66.1.69
Wilson V.S., Bobseine K., Lambright C.R., Gray L.E. Jr.
A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists.
Toxicol. Sci. 66:69-81(2002)

PubMed=20821581; DOI=10.1002/etc.166
Blake L.S., Martinovic D., Gray L.E. Jr., Wilson V.S., Regal R.R., Villeneuve D.L., Ankley G.T.
Characterization of the androgen-sensitive MDA-kb2 cell line for assessing complex environmental mixtures.
Environ. Toxicol. Chem. 29:1367-1376(2010)

Cross-references
Cell line collections ATCC; CRL-2713
Ontologies CLO; CLO_0007630