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Cellosaurus DiFi (CVCL_6895)

[Text version]

Cell line name DiFi
Synonyms DIFI
Accession CVCL_6895
Resource Identification Initiative To cite this cell line use: DiFi (RRID:CVCL_6895)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Doubling time: ~40 hours (PubMed=8385096).
Microsatellite instability: Stable (MSS) (PubMed=25926053; Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Transcriptome analysis.
Miscellaneous: Formerly the CCRID database had an entry describing this cell line (3111C0002000000030). It was one of the sources for the STR profile of this entry.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu443fs*16 (c.1328_1329delAA); Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 583; APC; Simple; p.Glu1151Ter (c.3451G>T); Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Lys132Arg (c.395A>G); ClinVar=VCV000376625; Zygosity=Homozygous (Cosmic-CLP; DepMap).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.44
East Asian, North1.85
East Asian, South0
South Asian0
European, North54.25
European, South40.55
Disease Colorectal carcinoma (NCIt: C2955)
Gardner syndrome (NCIt: C6728)
Gardner syndrome (ORDO: Orphanet_79665)
Derived from metastatic site: Ascites.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A2BW (DiFi R1 Cetux )CVCL_A2BX (DiFi R2 Cetux )
Sex of cell Female
Age at sampling 46Y
Category Cancer cell line
STR profile Source(s): CCRID; Cosmic-CLP; PubMed=25926053

Penta D12
Penta E12,18

Run an STR similarity search on this cell line

Boman B.M., Olive M., Untawale S., Blick M., North S., Gallick C., Dolf G., Siciliano M.J., Roubein L.D., Fritche H., Thomas G., Pathak S., Wildrick D.M., Levin B.
The DiFi rectal carcinoma cell line from a familial adenomatous polyposis patient: an in vitro model system to study genetic changes in colorectal cancer.
(In) Hereditary colorectal cancer; Utsunomiya J., Lynch H.T. (eds.); pp.541-548; Springer; Tokyo (1990)

PubMed=8385096; DOI=10.1007/BF02634191
Olive M., Untawale S., Coffey R.J., Siciliano M.J., Wildrick D.M., Fritsche H., Pathak S., Cherry L.M., Blick M., Lointier P., Roubein L.D., Levin B., Boman B.M.
Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient.
In Vitro Cell. Dev. Biol. Anim. 29:239-248(1993)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=22270724; DOI=10.1038/nm.2609
Montagut C., Dalmases A., Bellosillo B., Crespo M., Pairet S., Iglesias M., Salido M., Gallen M., Marsters S., Tsai S.P., Minoche A., Seshagiri S., Serrano S., Himmelbauer H., Bellmunt J., Rovira A., Settleman J., Bosch F., Albanell J.
Identification of a mutation in the extracellular domain of the epidermal growth factor receptor conferring cetuximab resistance in colorectal cancer.
Nat. Med. 18:221-223(2012)

PubMed=22722830; DOI=10.1038/nature11156
Misale S., Yaeger R., Hobor S., Scala E., Janakiraman M., Liska D., Valtorta E., Schiavo R., Buscarino M., Siravegna G., Bencardino K., Cercek A., Chen C.-T., Veronese S., Zanon C., Sartore-Bianchi A., Gambacorta M., Gallicchio M., Vakiani E., Boscaro V., Medico E., Weiser M., Siena S., Di Nicolantonio F., Solit D., Bardelli A.
Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer.
Nature 486:532-536(2012)

PubMed=25623215; DOI=10.1158/1078-0432.CCR-14-2821
Arena S., Bellosillo B., Siravegna G., Martinez A., Canadas I., Lazzari L., Ferruz N., Russo M., Misale S., Gonzalez I., Iglesias M., Gavilan E., Corti G., Hobor S., Crisafulli G., Salido M., Sanchez J., Dalmases A., Bellmunt J., De Fabritiis G., Rovira A., Di Nicolantonio F., Albanell J., Bardelli A., Montagut C.
Emergence of multiple EGFR extracellular mutations during cetuximab treatment in colorectal cancer.
Clin. Cancer Res. 21:2157-2166(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28854368; DOI=10.1016/j.celrep.2017.08.010
Roumeliotis T.I., Williams S.P., Goncalves E., Alsinet C., Del Castillo Velasco-Herrera M., Aben N., Ghavidel F.Z., Michaut M., Schubert M., Price S., Wright J.C., Yu L., Yang M., Dienstmann R., Guinney J., Beltrao P., Brazma A., Pardo M., Stegle O., Adams D.J., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.
Genomic determinants of protein abundance variation in colorectal cancer cells.
Cell Rep. 20:2201-2214(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

Cell line collections KCB; KCB 2014033YJ
Cell line databases/resources Cell_Model_Passport; SIDM00049
Cosmic-CLP; 1789883
DepMap; ACH-002233
Anatomy/cell type resources BTO; BTO:0005902
Chemistry resources GDSC; 1789883
PharmacoDB; DIFI_291_2019
Encyclopedic resources Wikidata; Q54831091
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM1448175
GEO; GSM1669732
Polymorphism and mutation databases Cosmic; 1479628
Cosmic; 1519358
Cosmic; 2841360
Proteomic databases PRIDE; PXD005235
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update16-Dec-2021
Version number24